Restriction map for
pF1KSDA0390
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# NO CUT ENZYMES ( 69 enzymes) #
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AatII AccI AclI AgeI AscI
AseI AsnI AspI AvrII BamHI
BbeI BlnI BsaBI BsiWI BsmBI
BsmI Bsp1407I BspDI BspLU11I BsrGI
Bst1107I BstBI BstEII BstPI BstZ17I
ClaI CpoI DraI EcoO65I EcoRI
EcoRV EcoT22I FseI HpaI KasI
KspI MamI MfeI MluI MunI
NarI NdeI NheI NotI NsiI
NspV PacI PflFI PinAI PmeI
PshAI PshBI Psp1406I PvuI RsrII
SacII SalI ScaI SfoI SfuI
SgrAI SnaBI SpeI SphI SspBI
SspI StuI SwaI Tth111I
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# ONE CUT ENZYMES ( 32 enzymes) #
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Acc65I 1286 GGTACC G^GTACC
AccIII 2279 TCCGGA T^CCGGA
AflII 2296 CTTAAG C^TTAAG
AflIII 1697 ACGTGT A^C[GA][CT]GT
AhdI 2029 GACCAGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 3444 GATCC GGATC(4/5)
Asp718I 1286 GGTACC G^GTACC
AspEI 2029 GACCAGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbvCI 3588 CCTCAGC CCTCAGC(-5/-2)
BcgI 3782 CGATGAACATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 2312 GGATAC GTATCC(6/5)
BclI 3203 TGATCA T^GATCA
BfaI 3214 CTAG C^TAG
BfrI 2296 CTTAAG C^TTAAG
BseAI 2279 TCCGGA T^CCGGA
BspEI 2279 TCCGGA T^CCGGA
BspHI 1313 TCATGA T^CATGA
BssHII 612 GCGCGC G^CGCGC
DrdI 2767 GACAGTTTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 2029 GACCAGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FbaI 3203 TGATCA T^GATCA
HincII 3115 GTCAAC GT[CT]^[GA]AC
HindII 3115 GTCAAC GT[CT]^[GA]AC
KpnI 1286 GGTACC GGTAC^C
MaeI 3214 CTAG C^TAG
MroI 2279 TCCGGA T^CCGGA
NruI 2223 TCGCGA TCG^CGA
PaeR7I 1846 CTCGAG C^TCGAG
RcaI 1313 TCATGA T^CATGA
SexAI 1931 ACCAGGT A^CC[AT]GGT
XbaI 3213 TCTAGA T^CTAGA
XhoI 1846 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 23 enzymes) #
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AcsI 2736 GAATTT [GA]^AATT[CT]
3861 AAATTC
ApaI 996 GGGCCC GGGCC^C
3760 GGGCCC
ApoI 2736 GAATTT [GA]^AATT[CT]
3861 AAATTC
Asp700I 465 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2271 GAAAACTTTC
AviII 446 TGCGCA TGC^GCA
1091 TGCGCA
BglII 1127 AGATCT A^GATCT
2191 AGATCT
BsrDI 2991 CATTGC GCAATG(2/0)
3489 CATTGC
BstYI 1127 AGATCT [GA]^GATC[CT]
2191 AGATCT
EcoNI 552 CCTGGGCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2888 CCTCCCAGAGG
FspI 446 TGCGCA TGC^GCA
1091 TGCGCA
HindIII 2483 AAGCTT A^AGCTT
2756 AAGCTT
MflI 1127 AGATCT [GA]^GATC[CT]
2191 AGATCT
SapI 2702 GAAGAGC GCTCTTC(1/4)
3273 GAAGAGC
SbfI 723 CCTGCAGG CCTGCA^GG
1857 CCTGCAGG
SfiI 696 GGCCCCGCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
975 GGCCGAGTCGGCC
SmaI 2961 CCCGGG CCC^GGG
3134 CCCGGG
Sse8387I 723 CCTGCAGG CCTGCA^GG
1857 CCTGCAGG
TfiI 522 GATTC G^A[AT]TC
2540 GAATC
Tsp509I 2737 AATT ^AATT
3862 AATT
XhoII 1127 AGATCT [GA]^GATC[CT]
2191 AGATCT
XmaCI 2961 CCCGGG C^CCGGG
3134 CCCGGG
XmaI 2961 CCCGGG C^CCGGG
3134 CCCGGG
XmnI 465 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2271 GAAAACTTTC
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# 3 OR MORE CUT ENZYMES ( 134 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
Alw44I AlwNI AocI Aor51HI ApaLI
AspHI AvaI AvaII BalI BanI
BanII BbrPI BbsI BbvI BcnI
BglI BlpI BmyI BpmI Bpu1102I
BpuAI BsaAI BsaHI BsaI BsaJI
BsaWI BseRI BsgI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrBI BsrFI BsrI
BssKI BssSI BstAPI BstF5I BstNI
BstUI BstXI Bsu36I Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DraIII DsaI EaeI EagI
EarI EclXI Eco47III Eco52I Eco81I
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeII MaeIII
MboI MboII MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NaeI NciI NcoI
NdeII NgoMIV NlaIII NlaIV NspI
PflMI PleI PmaCI PmlI PpuMI
PstI PvuII RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TaqI Tru9I TseI Tsp45I
TspRI TthHB8I Van91I XcmI