Restriction map for
pF1KSDA0414
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# NO CUT ENZYMES ( 112 enzymes) #
##################################
Acc65I AclI AflII AflIII AgeI
AocI ApaI AscI AseI AsnI
Asp718I AspI AvaII AviII AvrII
BalI BbvCI BclI BfrI BglI
BglII BlnI BsaI BsiEI BsiWI
BsmI BspDI BspHI BspLU11I BsrDI
BssHII Bst1107I BstBI BstEII BstPI
BstZ17I Bsu36I ClaI CpoI DraI
DraII DrdI EaeI EagI EclXI
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HgaI HincII HindII HpaI
KpnI KspI MfeI MluI MluNI
MscI MunI NaeI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmeI
PpuMI PshAI PshBI Psp1406I PstI
PvuI PvuII RcaI RsrII SacI
SacII SalI SbfI ScaI SfiI
SfuI SgrAI SmaI SmlI SnaBI
SpeI Sse8387I StuI SwaI TaqI
Tth111I TthHB8I XbaI XcmI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 65 enzymes) #
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AccI 346 GTAGAC GT^[AC][GT]AC
AccII 783 CGCG CG^CG
AccIII 976 TCCGGA T^CCGGA
Alw44I 778 GTGCAC G^TGCAC
Aor51HI 746 AGCGCT AGC^GCT
ApaLI 778 GTGCAC G^TGCAC
Asp700I 1134 GAAAAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 742 CCCGAG C^[CT]CG[GA]G
BamHI 728 GGATCC G^GATCC
BanI 652 GGCGCC G^G[CT][GA]CC
BanII 471 GGGCTC G[GA]GC[CT]^C
BbeI 652 GGCGCC GGCGC^C
BbrPI 733 CACGTG CAC^GTG
BbsI 1187 GTCTTC GAAGAC(2/6)
BcgI 1175 GCATGCACCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1116 GTATCC GTATCC(6/5)
BlpI 1375 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1375 GCTGAGC GC^T[ACGT]AGC
BpuAI 1187 GTCTTC GAAGAC(2/6)
BsaAI 733 CACGTG [CT]AC^GT[GA]
BsaBI 991 GATGGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 976 TCCGGA [AT]^CCGG[AT]
BseAI 976 TCCGGA T^CCGGA
BsmAI 103 GTCTC GTCTC(1/5)
BsmBI 102 CGTCTC CGTCTC(1/5)
BsmFI 1319 GGGAC GGGAC(10/14)
BsoBI 742 CCCGAG C^[CT]CG[GA]G
Bsp1407I 692 TGTACA T^GTACA
BspEI 976 TCCGGA T^CCGGA
BsrBI 287 CCGCTC CCGCTC(-3/-3)
BsrFI 1331 ACCGGC [GA]^CCGG[CT]
BsrGI 692 TGTACA T^GTACA
BssSI 1243 CTCGTG CACGAG(-5/-1)
BstAPI 837 GCACACGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 783 CGCG CG^CG
BstXI 704 CCAGCATCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 728 GGATCC [GA]^GATC[CT]
CelII 1375 GCTGAGC GC^T[ACGT]AGC
Cfr10I 1331 ACCGGC [GA]^CCGG[CT]
DraIII 838 CACACGGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 962 CCATGG C^C[GA][CT]GG
Eco47III 746 AGCGCT AGC^GCT
FauI 286 CCCGC CCCGC(4/6)
KasI 652 GGCGCC G^GCGCC
MamI 991 GATGGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 728 GGATCC [GA]^GATC[CT]
MroI 976 TCCGGA T^CCGGA
MseI 1070 TTAA T^TAA
MspA1I 314 CAGCGG C[AC]G^C[GT]G
MvnI 783 CGCG CG^CG
NarI 652 GGCGCC GG^CGCC
NcoI 962 CCATGG C^CATGG
PflMI 1239 CCATCTCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 733 CACGTG CAC^GTG
PmlI 733 CACGTG CAC^GTG
SapI 956 GCTCTTC GCTCTTC(1/4)
SfaNI 707 GCATC GCATC(5/9)
SfoI 652 GGCGCC GGC^GCC
SphI 1175 GCATGC GCATG^C
SspBI 692 TGTACA T^GTACA
SspI 1048 AATATT AAT^ATT
Tru9I 1070 TTAA T^TAA
Van91I 1239 CCATCTCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 728 GGATCC [GA]^GATC[CT]
XmnI 1134 GAAAAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 26 enzymes) #
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AatII 100 GACGTC GACGT^C
272 GACGTC
AhdI 415 GACCACCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
547 GACGAGCTGTC
AlwI 729 GATCC GGATC(4/5)
728 GGATC
AlwNI 61 CAGAAACTG CAG[ACGT][ACGT][ACGT]^CTG
1100 CAGCCACTG
AspEI 415 GACCACCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
547 GACGAGCTGTC
BcnI 132 CCGGG CC^[GC]GG
683 CCCGG
BfaI 260 CTAG C^TAG
277 CTAG
BpmI 290 CTCCAG CTGGAG(16/14)
328 CTCCAG
BsgI 826 GTGCAG GTGCAG(16/14)
844 GTGCAG
BsrI 158 CCAGT ACTGG(1/-1)
420 CCAGT
Cfr13I 686 GGCCC G^G[ACGT]CC
1248 GGGCC
DpnII 729 GATC ^GATC
1162 GATC
Eam1105I 415 GACCACCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
547 GACGAGCTGTC
EcoT14I 117 CCAAGG C^C[AT][AT]GG
962 CCATGG
HindIII 321 AAGCTT A^AGCTT
1079 AAGCTT
MaeI 260 CTAG C^TAG
277 CTAG
MboI 729 GATC ^GATC
1162 GATC
NciI 132 CCGGG CC^[GC]GG
683 CCCGG
NdeII 729 GATC ^GATC
1162 GATC
PleI 811 GAGTC GAGTC(4/5)
1152 GAGTC
Sau3AI 729 GATC ^GATC
1162 GATC
Sau96I 686 GGCCC G^G[ACGT]CC
1248 GGGCC
SexAI 179 ACCAGGT A^CC[AT]GGT
800 ACCAGGT
SfcI 58 CTACAG C^T[GA][CT]AG
380 CTACAG
StyI 117 CCAAGG C^C[AT][AT]GG
962 CCATGG
TfiI 996 GAATC G^A[AT]TC
1094 GATTC
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# 3 OR MORE CUT ENZYMES ( 55 enzymes) #
#########################################
AciI AcsI AcyI AfaI AluI
ApoI AspHI BbvI BmyI BsaHI
BsaJI BseRI BsiHKAI BsiYI BslI
Bsp1286I BspMI BssKI BstF5I BstNI
Cac8I CfoI DdeI EarI EcoRII
Fnu4HI FokI HaeII HaeIII HapII
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeII MaeIII
MboII MnlI MslI MspI MvaI
MwoI NlaIII NlaIV NspI RsaI
ScrFI TseI Tsp45I Tsp509I TspRI