Restriction map for
pF1KSDA0422
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# NO CUT ENZYMES ( 80 enzymes) #
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AatII Acc65I AclI AcsI AflII
AgeI AhdI Aor51HI ApoI AscI
AseI AsnI Asp718I AspEI AvrII
BbrPI BfrI BlnI BsiWI BsmBI
BsmI Bsp1407I BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstZ17I CpoI
DraI DraIII DrdI EagI Eam1105I
EarI EclXI Eco47III Eco52I EcoRI
EcoRV EcoT22I FseI HindIII HpaI
KpnI Ksp632I MfeI MunI NaeI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RsrII SapI SbfI
SfuI SgrAI SnaBI SpeI Sse8387I
SspBI SspI SwaI XbaI XhoI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AccIII 212 TCCGGA T^CCGGA
Asp700I 384 GAAGCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 1126 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 1188 TGCGCA TGC^GCA
BamHI 1995 GGATCC G^GATCC
BciVI 276 GGATAC GTATCC(6/5)
BclI 1911 TGATCA T^GATCA
BlpI 2578 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 2578 GCTCAGC GC^T[ACGT]AGC
BsaBI 1266 GATCAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 212 TCCGGA T^CCGGA
BsiEI 3210 CGACCG CG[GA][CT]^CG
BspDI 1894 ATCGAT AT^CGAT
BspEI 212 TCCGGA T^CCGGA
BspHI 1202 TCATGA T^CATGA
BspLU11I 2279 ACATGT A^CATGT
BstEII 3222 GGTGACC G^GT[ACGT]ACC
BstPI 3222 GGTGACC G^GT[ACGT]ACC
CelII 2578 GCTCAGC GC^T[ACGT]AGC
ClaI 1894 ATCGAT AT^CGAT
EcoNI 3234 CCTGTACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 3222 GGTGACC G^GT[ACGT]ACC
FbaI 1911 TGATCA T^GATCA
FspI 1188 TGCGCA TGC^GCA
HincII 2592 GTCGAC GT[CT]^[GA]AC
HindII 2592 GTCGAC GT[CT]^[GA]AC
KspI 963 CCGCGG CCGC^GG
MamI 1266 GATCAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI 1616 ACGCGT A^CGCGT
MroI 212 TCCGGA T^CCGGA
MseI 833 TTAA T^TAA
PflFI 1126 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
RcaI 1202 TCATGA T^CATGA
SacI 1216 GAGCTC GAGCT^C
SacII 963 CCGCGG CCGC^GG
SalI 2592 GTCGAC G^TCGAC
ScaI 1979 AGTACT AGT^ACT
SexAI 3239 ACCAGGT A^CC[AT]GGT
Tru9I 833 TTAA T^TAA
Tsp509I 3079 AATT ^AATT
Tth111I 1126 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmnI 384 GAAGCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 20 enzymes) #
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AflIII 1616 ACGCGT A^C[GA][CT]GT
2279 ACATGT
Alw44I 1182 GTGCAC G^TGCAC
1438 GTGCAC
AlwNI 1192 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
1564 CAGTACCTG
ApaLI 1182 GTGCAC G^TGCAC
1438 GTGCAC
BcgI 996 GCATGAGAATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1707 GCAGCGGACTCG
BglII 1106 AGATCT A^GATCT
1271 AGATCT
BsaAI 637 TACGTG [CT]AC^GT[GA]
1382 TACGTG
BsaI 958 GAGACC GGTCTC(1/5)
2476 GAGACC
BsaWI 212 TCCGGA [AT]^CCGG[AT]
2672 ACCGGA
BsrFI 596 ACCGGC [GA]^CCGG[CT]
1937 GCCGGT
Cfr10I 596 ACCGGC [GA]^CCGG[CT]
1937 GCCGGT
PpuMI 2406 GGGTCCC [GA]G^G[AT]CC[CT]
3203 GGGTCCC
SfiI 1525 GGCCGGGCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
1689 GGCCATGCTGGCC
SmaI 1316 CCCGGG CCC^GGG
2726 CCCGGG
SmlI 1624 CTCAAG C^T[CT][GA]AG
1969 CTTGAG
SphI 767 GCATGC GCATG^C
2574 GCATGC
StuI 947 AGGCCT AGG^CCT
1790 AGGCCT
XcmI 3029 CCATGCGGCTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3084 CCAGATGAAGATTGG
XmaCI 1316 CCCGGG C^CCGGG
2726 CCCGGG
XmaI 1316 CCCGGG C^CCGGG
2726 CCCGGG
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# 3 OR MORE CUT ENZYMES ( 116 enzymes) #
#########################################
AccI AccII AciI AcyI AfaI
AluI AlwI AocI ApaI AspHI
AvaI AvaII BalI BanI BanII
BbeI BbsI BbvCI BbvI BcnI
BfaI BglI BmyI BpmI BpuAI
BsaHI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrBI BsrDI BsrI
BssKI BstF5I BstNI BstUI BstXI
BstYI Bsu36I Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
Eco81I EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI KasI MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MslI MspA1I
MspI MvaI MvnI MwoI NarI
NciI NcoI NdeII NlaIII NlaIV
NspI PflMI PleI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SfoI StyI TaqI TfiI
TseI Tsp45I TspRI TthHB8I Van91I
XhoII