Restriction map for
pF1KSDA0426
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# NO CUT ENZYMES ( 133 enzymes) #
##################################
AatII Acc65I AccII AccIII AciI
AclI AflII AflIII AgeI Aor51HI
ApaI AscI AseI AsnI Asp718I
AspI AvaI AviII AvrII BalI
BbrPI BciVI BclI BcnI BfrI
BglI BlnI BlpI Bpu1102I BsaAI
BsaBI BsaWI BseAI BsiEI BsiWI
BsoBI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrBI BsrDI BsrGI BssHII
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstXI BstZ17I CelII ClaI
CpoI DrdI EagI EclXI Eco47III
Eco52I EcoO65I EcoRV EcoT14I EcoT22I
FauI FbaI FseI FspI HgaI
HincII HindII HpaI KpnI KspI
MamI MfeI MluI MluNI MroI
MscI MspA1I MunI MvnI NaeI
NciI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PstI PvuI PvuII RcaI RsrII
SacII SalI SbfI SexAI SfcI
SfiI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StyI SwaI Tth111I XbaI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AccI 452 GTCTAC GT^[AC][GT]AC
AcyI 1228 GGCGCC G[GA]^CG[CT]C
Alw44I 1161 GTGCAC G^TGCAC
AocI 295 CCTGAGG CC^T[ACGT]AGG
ApaLI 1161 GTGCAC G^TGCAC
BamHI 881 GGATCC G^GATCC
BanI 1228 GGCGCC G^G[CT][GA]CC
BbeI 1228 GGCGCC GGCGC^C
BbvCI 222 GCTGAGG CCTCAGC(-5/-2)
BcgI 202 CGAGAACTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 662 CTAG C^TAG
BglII 1806 AGATCT A^GATCT
BsaHI 1228 GGCGCC G[GA]^CG[CT]C
BsgI 316 GTGCAG GTGCAG(16/14)
BsmBI 442 GAGACG CGTCTC(1/5)
BsmI 1593 GAATGC GAATGC(1/-1)
BssSI 677 CTCGTG CACGAG(-5/-1)
Bsu36I 295 CCTGAGG CC^T[ACGT]AGG
CfoI 1229 GCGC GCG^C
DraI 582 TTTAAA TTT^AAA
DraIII 1164 CACTCAGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 933 CCGTGG C^C[GA][CT]GG
EaeI 1017 TGGCCG [CT]^GGCC[GA]
Eco81I 295 CCTGAGG CC^T[ACGT]AGG
EcoNI 291 CCTACCTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HaeII 1228 GGCGCC [GA]GCGC^[CT]
HhaI 1229 GCGC GCG^C
Hin1I 1228 GGCGCC G[GA]^CG[CT]C
HinP1I 1229 GCGC G^CGC
KasI 1228 GGCGCC G^GCGCC
MaeI 662 CTAG C^TAG
MslI 1075 CACTGTAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI 1228 GGCGCC GG^CGCC
PflMI 331 CCAGAGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 197 GACTC GAGTC(4/5)
PpuMI 95 GGGACCT [GA]G^G[AT]CC[CT]
SapI 700 GAAGAGC GCTCTTC(1/4)
ScaI 140 AGTACT AGT^ACT
SfoI 1228 GGCGCC GGC^GCC
TaqI 954 TCGA T^CGA
TthHB8I 954 TCGA T^CGA
Van91I 331 CCAGAGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 162 CCAGGAGACATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AfaI 141 GTAC GT^AC
448 GTAC
AhdI 168 GACATCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
444 GACGGTACGTC
AspEI 168 GACATCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
444 GACGGTACGTC
BbvI 324 GCAGC GCAGC(8/12)
261 GCTGC
BsaI 232 GAGACC GGTCTC(1/5)
402 GGTCTC
BseRI 298 GAGGAG GAGGAG(10/8)
343 GAGGAG
BspMI 98 ACCTGC ACCTGC(4/8)
399 GCAGGT
BsrFI 1019 GCCGGT [GA]^CCGG[CT]
1225 ACCGGC
Cac8I 712 GCTTGC GC[ACGT]^[ACGT]GC
1111 GCAGGC
Cfr10I 1019 GCCGGT [GA]^CCGG[CT]
1225 ACCGGC
DraII 95 GGGACCT [GA]G^G[ACGT]CC[CT]
1567 AGGCCCT
Eam1105I 168 GACATCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
444 GACGGTACGTC
EarI 67 GAAGAG CTCTTC(1/4)
700 GAAGAG
EcoO109I 95 GGGACCT [GA]G^G[ACGT]CC[CT]
1567 AGGCCCT
EcoRI 1382 GAATTC G^AATTC
1466 GAATTC
Fnu4HI 261 GCTGC GC^[ACGT]GC
324 GCAGC
HapII 1020 CCGG C^CGG
1226 CCGG
HindIII 842 AAGCTT A^AGCTT
926 AAGCTT
HpaII 1020 CCGG C^CGG
1226 CCGG
ItaI 261 GCTGC GC^[ACGT]GC
324 GCAGC
Ksp632I 67 GAAGAG CTCTTC(1/4)
700 GAAGAG
MaeII 450 ACGT A^CGT
1415 ACGT
MaeIII 352 GTGAC ^GT[ACGT]AC
1412 GTGAC
MseI 583 TTAA T^TAA
1034 TTAA
MspI 1020 CCGG C^CGG
1226 CCGG
RsaI 141 GTAC GT^AC
448 GTAC
SacI 301 GAGCTC GAGCT^C
1767 GAGCTC
StuI 308 AGGCCT AGG^CCT
1113 AGGCCT
Tru9I 583 TTAA T^TAA
1034 TTAA
TseI 261 GCTGC G^C[AT]GC
324 GCAGC
Tsp45I 352 GTGAC ^GT[GC]AC
1412 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 51 enzymes) #
#########################################
AcsI AluI AlwI AlwNI ApoI
Asp700I AspHI AvaII BanII BbsI
BmyI BpmI BpuAI BsaJI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrI BssKI BstF5I BstNI BstYI
Cfr13I DdeI DpnII EcoRII FokI
HaeIII HinfI HphI MboI MboII
MflI MnlI MvaI MwoI NdeII
NlaIII NlaIV Sau3AI Sau96I ScrFI
SfaNI TfiI Tsp509I TspRI XhoII
XmnI