Restriction map for
pF1KSDA0463
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# NO CUT ENZYMES ( 46 enzymes) #
##################################
Acc65I AclI AgeI AscI Asp700I
Asp718I AvrII BbsI BciVI BlnI
BpuAI BsaBI BsiWI BssHII BstBI
CpoI DraI EcoRI FseI HpaI
KpnI KspI MamI MluI NdeI
NheI NotI NruI NspV PacI
PinAI PmeI PshAI Psp1406I PvuI
RsrII SacII ScaI SexAI SfuI
SgrAI SnaBI SpeI SwaI XbaI
XmnI
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# ONE CUT ENZYMES ( 36 enzymes) #
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AccIII 4325 TCCGGA T^CCGGA
AcsI 3274 AAATTC [GA]^AATT[CT]
AflII 2209 CTTAAG C^TTAAG
ApoI 3274 AAATTC [GA]^AATT[CT]
AseI 5704 ATTAAT AT^TAAT
AsnI 5704 ATTAAT AT^TAAT
AviII 4402 TGCGCA TGC^GCA
BamHI 3263 GGATCC G^GATCC
BcgI 1095 CGATGACTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 2209 CTTAAG C^TTAAG
BseAI 4325 TCCGGA T^CCGGA
BsmBI 19 CGTCTC CGTCTC(1/5)
BsmI 5532 GAATGC GAATGC(1/-1)
Bsp1407I 1316 TGTACA T^GTACA
BspEI 4325 TCCGGA T^CCGGA
BsrGI 1316 TGTACA T^GTACA
BssSI 2809 CTCGTG CACGAG(-5/-1)
BstAPI 2078 GCAACCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
EcoRV 3940 GATATC GAT^ATC
EcoT22I 4798 ATGCAT ATGCA^T
FspI 4402 TGCGCA TGC^GCA
HindIII 3917 AAGCTT A^AGCTT
MfeI 4362 CAATTG C^AATTG
MroI 4325 TCCGGA T^CCGGA
MunI 4362 CAATTG C^AATTG
NsiI 4798 ATGCAT ATGCA^T
PaeR7I 3836 CTCGAG C^TCGAG
PshBI 5704 ATTAAT AT^TAAT
SalI 3115 GTCGAC G^TCGAC
SapI 588 GCTCTTC GCTCTTC(1/4)
SfiI 4222 GGCCTGCAGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SphI 146 GCATGC GCATG^C
SspBI 1316 TGTACA T^GTACA
SspI 2131 AATATT AAT^ATT
XcmI 1556 CCAGGGTCCCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 3836 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 33 enzymes) #
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AatII 1213 GACGTC GACGT^C
4890 GACGTC
AhdI 313 GACAACAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
661 GACCCTGAGTC
AocI 832 CCTGAGG CC^T[ACGT]AGG
2425 CCTCAGG
Aor51HI 1520 AGCGCT AGC^GCT
5164 AGCGCT
AspEI 313 GACAACAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
661 GACCCTGAGTC
BbrPI 3688 CACGTG CAC^GTG
4084 CACGTG
BbvCI 671 CCTCAGC CCTCAGC(-5/-2)
1953 GCTGAGG
BclI 1515 TGATCA T^GATCA
4964 TGATCA
BglI 2243 GCCAGCGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
4223 GCCTGCAGGGC
BsaWI 2498 ACCGGA [AT]^CCGG[AT]
4325 TCCGGA
BspLU11I 1649 ACATGT A^CATGT
5189 ACATGT
BsrDI 3198 CATTGC GCAATG(2/0)
3816 CATTGC
Bst1107I 1584 GTATAC GTA^TAC
4923 GTATAC
BstZ17I 1584 GTATAC GTA^TAC
4923 GTATAC
Bsu36I 832 CCTGAGG CC^T[ACGT]AGG
2425 CCTCAGG
DraIII 2719 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG
5563 CACGCCGTG
EagI 3773 CGGCCG C^GGCCG
4678 CGGCCG
Eam1105I 313 GACAACAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
661 GACCCTGAGTC
EclXI 3773 CGGCCG C^GGCCG
4678 CGGCCG
Eco47III 1520 AGCGCT AGC^GCT
5164 AGCGCT
Eco52I 3773 CGGCCG C^GGCCG
4678 CGGCCG
Eco81I 832 CCTGAGG CC^T[ACGT]AGG
2425 CCTCAGG
FbaI 1515 TGATCA T^GATCA
4964 TGATCA
NaeI 142 GCCGGC GCC^GGC
3775 GCCGGC
NcoI 1437 CCATGG C^CATGG
2679 CCATGG
NgoMIV 142 GCCGGC G^CCGGC
3775 GCCGGC
PflMI 1275 CCAGCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2570 CCAGCCCCTGG
PmaCI 3688 CACGTG CAC^GTG
4084 CACGTG
PmlI 3688 CACGTG CAC^GTG
4084 CACGTG
SbfI 972 CCTGCAGG CCTGCA^GG
4224 CCTGCAGG
Sse8387I 972 CCTGCAGG CCTGCA^GG
4224 CCTGCAGG
StuI 1016 AGGCCT AGG^CCT
1679 AGGCCT
Van91I 1275 CCAGCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2570 CCAGCCCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 143 enzymes) #
#########################################
AccI AccII AciI AcyI AfaI
AflIII AluI Alw44I AlwI AlwNI
ApaI ApaLI AspHI AspI AvaI
AvaII BalI BanI BanII BbeI
BbvI BcnI BfaI BglII BlpI
BmyI BpmI Bpu1102I BsaAI BsaHI
BsaI BsaJI BseRI BsgI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspDI BspHI BspMI
BsrBI BsrFI BsrI BssKI BstEII
BstF5I BstNI BstPI BstUI BstXI
BstYI Cac8I CelII CfoI Cfr10I
Cfr13I ClaI DdeI DpnII DraII
DrdI DsaI EaeI EarI EcoNI
EcoO109I EcoO65I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HincII
HindII HinfI HpaII HphI ItaI
KasI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NarI NciI
NdeII NlaIII NlaIV NspI PflFI
PleI PpuMI PstI PvuII RcaI
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SfoI SmaI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI Tth111I TthHB8I
XhoII XmaCI XmaI