Restriction map for
pF1KSDA0466
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# NO CUT ENZYMES ( 86 enzymes) #
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AatII AccI AclI AflIII AgeI
AhdI Alw44I Aor51HI ApaLI AscI
AseI AsnI AspEI AviII AvrII
BbeI BbvCI BcgI BclI BlnI
BsaBI BsiWI Bsp1407I BspDI BspHI
BspLU11I BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstZ17I ClaI CpoI
DraI EagI Eam1105I EclXI Eco47III
Eco52I EcoRV FbaI FseI FspI
HpaI KasI MamI MfeI MluI
MunI NaeI NarI NdeI NgoMIV
NheI NotI NruI NspV PacI
PaeR7I PinAI PmeI PshAI PshBI
Psp1406I PvuI RcaI RsrII SalI
SapI ScaI SexAI SfiI SfoI
SfuI SgrAI SnaBI SpeI SphI
SspBI SspI StuI SwaI TfiI
XhoI
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# ONE CUT ENZYMES ( 36 enzymes) #
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Acc65I 3278 GGTACC G^GTACC
AccIII 762 TCCGGA T^CCGGA
AflII 1045 CTTAAG C^TTAAG
ApaI 3108 GGGCCC GGGCC^C
Asp718I 3278 GGTACC G^GTACC
AspI 1066 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbrPI 2347 CACGTG CAC^GTG
BciVI 3560 GTATCC GTATCC(6/5)
BfrI 1045 CTTAAG C^TTAAG
BsaAI 2347 CACGTG [CT]AC^GT[GA]
BsaWI 762 TCCGGA [AT]^CCGG[AT]
BseAI 762 TCCGGA T^CCGGA
BsiEI 930 CGACCG CG[GA][CT]^CG
BsmBI 2704 CGTCTC CGTCTC(1/5)
BspEI 762 TCCGGA T^CCGGA
EcoT22I 903 ATGCAT ATGCA^T
HaeII 1586 AGCGCC [GA]GCGC^[CT]
HincII 814 GTCAAC GT[CT]^[GA]AC
HindII 814 GTCAAC GT[CT]^[GA]AC
HindIII 2182 AAGCTT A^AGCTT
KpnI 3278 GGTACC GGTAC^C
KspI 264 CCGCGG CCGC^GG
MroI 762 TCCGGA T^CCGGA
MseI 1046 TTAA T^TAA
NsiI 903 ATGCAT ATGCA^T
PflFI 1066 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 2347 CACGTG CAC^GTG
PmlI 2347 CACGTG CAC^GTG
SacI 490 GAGCTC GAGCT^C
SacII 264 CCGCGG CCGC^GG
SbfI 2856 CCTGCAGG CCTGCA^GG
Sse8387I 2856 CCTGCAGG CCTGCA^GG
Tru9I 1046 TTAA T^TAA
Tth111I 1066 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 3324 TCTAGA T^CTAGA
XcmI 756 CCAGGATCCGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 30 enzymes) #
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AcyI 1447 GGCGTC G[GA]^CG[CT]C
2617 GACGCC
AocI 2448 CCTGAGG CC^T[ACGT]AGG
2976 CCTGAGG
Asp700I 273 GAAGATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
791 GAAAGCGTTC
AvaI 335 CCCGGG C^[CT]CG[GA]G
1796 CCCGGG
BalI 1052 TGGCCA TGG^CCA
1244 TGGCCA
BamHI 752 GGATCC G^GATCC
759 GGATCC
BbsI 2193 GAAGAC GAAGAC(2/6)
3385 GTCTTC
BglI 2777 GCCCCAGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3128 GCCCAGAGGGC
BpuAI 2193 GAAGAC GAAGAC(2/6)
3385 GTCTTC
BsaHI 1447 GGCGTC G[GA]^CG[CT]C
2617 GACGCC
BsoBI 335 CCCGGG C^[CT]CG[GA]G
1796 CCCGGG
BsrDI 125 GCAATG GCAATG(2/0)
1250 GCAATG
BstXI 1997 CCAACCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3428 CCATCCTTCTGG
Bsu36I 2448 CCTGAGG CC^T[ACGT]AGG
2976 CCTGAGG
DraIII 466 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG
874 CACACGGTG
DrdI 1801 GACGGAGGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2866 GACACAGTGGTC
EaeI 1052 TGGCCA [CT]^GGCC[GA]
1244 TGGCCA
Eco81I 2448 CCTGAGG CC^T[ACGT]AGG
2976 CCTGAGG
EcoNI 645 CCTGGGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1095 CCTCCGCCAGG
Hin1I 1447 GGCGTC G[GA]^CG[CT]C
2617 GACGCC
MluNI 1052 TGGCCA TGG^CCA
1244 TGGCCA
MscI 1052 TGGCCA TGG^CCA
1244 TGGCCA
NcoI 221 CCATGG C^CATGG
980 CCATGG
NspI 2274 GCATGT [GA]CATG^[CT]
2786 GCATGT
SmaI 335 CCCGGG CCC^GGG
1796 CCCGGG
TaqI 1856 TCGA T^CGA
2053 TCGA
TthHB8I 1856 TCGA T^CGA
2053 TCGA
XmaCI 335 CCCGGG C^CCGGG
1796 CCCGGG
XmaI 335 CCCGGG C^CCGGG
1796 CCCGGG
XmnI 273 GAAGATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
791 GAAAGCGTTC
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# 3 OR MORE CUT ENZYMES ( 106 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI ApoI AspHI AvaII
BanI BanII BbvI BcnI BfaI
BglII BlpI BmyI BpmI Bpu1102I
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
Bsp1286I BspMI BsrBI BsrFI BsrI
BssKI BstEII BstF5I BstNI BstPI
BstUI BstYI Cac8I CelII CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EarI EcoO109I EcoO65I EcoRI
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV PflMI PleI PpuMI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TseI Tsp45I Tsp509I TspRI Van91I
XhoII