Restriction map for
pF1KSDA0537
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# NO CUT ENZYMES ( 106 enzymes) #
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AatII AccIII AcsI AflII AflIII
AgeI AocI ApoI AscI AseI
AsnI Asp700I AspI AviII AvrII
BbrPI BbvCI BciVI BfrI BglI
BlnI BlpI Bpu1102I BsaAI BseAI
BsiWI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstXI
BstZ17I Bsu36I CelII ClaI CpoI
DraI DrdI EagI EclXI Eco52I
Eco81I EcoRI EcoRV FseI FspI
HincII HindII HindIII HpaI KspI
MfeI MluI MroI MunI NaeI
NdeI NgoMIV NheI NotI NruI
NspI NspV PacI PflFI PinAI
PmaCI PmeI PmlI PpuMI PshBI
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI StuI SwaI
Tth111I XbaI XcmI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 57 enzymes) #
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Acc65I 1880 GGTACC G^GTACC
AccI 1945 GTCTAC GT^[AC][GT]AC
AccII 15 CGCG CG^CG
AclI 630 AACGTT AA^CGTT
AhdI 964 GACTCTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 496 GTGCAC G^TGCAC
Aor51HI 1956 AGCGCT AGC^GCT
ApaI 710 GGGCCC GGGCC^C
ApaLI 496 GTGCAC G^TGCAC
Asp718I 1880 GGTACC G^GTACC
AspEI 964 GACTCTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 1055 TGGCCA TGG^CCA
BamHI 1196 GGATCC G^GATCC
BbeI 54 GGCGCC GGCGC^C
BbsI 1458 GTCTTC GAAGAC(2/6)
BcgI 1764 GCAGGAGAATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 386 TGATCA T^GATCA
BfaI 1084 CTAG C^TAG
BglII 1964 AGATCT A^GATCT
BpuAI 1458 GTCTTC GAAGAC(2/6)
BsaWI 1883 ACCGGA [AT]^CCGG[AT]
BsgI 1305 GTGCAG GTGCAG(16/14)
BsrDI 897 CATTGC GCAATG(2/0)
BstEII 1533 GGTAACC G^GT[ACGT]ACC
BstPI 1533 GGTAACC G^GT[ACGT]ACC
BstUI 15 CGCG CG^CG
DraIII 118 CACGGGGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 1604 CCATGG C^C[GA][CT]GG
Eam1105I 964 GACTCTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 1437 CTCTTC CTCTTC(1/4)
Eco47III 1956 AGCGCT AGC^GCT
EcoO65I 1533 GGTAACC G^GT[ACGT]ACC
EcoT22I 653 ATGCAT ATGCA^T
FbaI 386 TGATCA T^GATCA
KasI 54 GGCGCC G^GCGCC
KpnI 1880 GGTACC GGTAC^C
Ksp632I 1437 CTCTTC CTCTTC(1/4)
MaeI 1084 CTAG C^TAG
MaeII 631 ACGT A^CGT
MluNI 1055 TGGCCA TGG^CCA
MscI 1055 TGGCCA TGG^CCA
MslI 1920 CACAGACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 15 CGCG CG^CG
NarI 54 GGCGCC GG^CGCC
NcoI 1604 CCATGG C^CATGG
NsiI 653 ATGCAT ATGCA^T
PaeR7I 836 CTCGAG C^TCGAG
PflMI 902 CCAACCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 1938 GACTCAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 630 AACGTT AA^CGTT
PvuI 873 CGATCG CGAT^CG
SfoI 54 GGCGCC GGC^GCC
SmlI 836 CTCGAG C^T[CT][GA]AG
SspI 571 AATATT AAT^ATT
Tsp509I 270 AATT ^AATT
Van91I 902 CCAACCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 836 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AcyI 54 GGCGCC G[GA]^CG[CT]C
443 GACGCC
AspHI 496 GTGCAC G[AT]GC[AT]^C
1318 GTGCTC
AvaI 68 CCCGAG C^[CT]CG[GA]G
836 CTCGAG
BsaBI 309 GATTGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
381 GATTGTGATC
BsaHI 54 GGCGCC G[GA]^CG[CT]C
443 GACGCC
BseRI 1321 CTCCTC GAGGAG(10/8)
1915 CTCCTC
BsiEI 873 CGATCG CG[GA][CT]^CG
1096 CGGTCG
BsiHKAI 496 GTGCAC G[AT]GC[AT]^C
1318 GTGCTC
BsmBI 486 CGTCTC CGTCTC(1/5)
1803 CGTCTC
BsoBI 68 CCCGAG C^[CT]CG[GA]G
836 CTCGAG
BsrBI 436 GAGCGG CCGCTC(-3/-3)
1087 GAGCGG
BsrFI 1853 ACCGGC [GA]^CCGG[CT]
1889 ACCGGC
Cfr10I 1853 ACCGGC [GA]^CCGG[CT]
1889 ACCGGC
EcoNI 682 CCTTACCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1553 CCTGCCGGAGG
EcoT14I 1190 CCAAGG C^C[AT][AT]GG
1604 CCATGG
HaeII 54 GGCGCC [GA]GCGC^[CT]
1956 AGCGCT
HgaI 443 GACGC GACGC(5/10)
1802 GCGTC
Hin1I 54 GGCGCC G[GA]^CG[CT]C
443 GACGCC
MamI 309 GATTGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
381 GATTGTGATC
MseI 272 TTAA T^TAA
575 TTAA
PvuII 705 CAGCTG CAG^CTG
1797 CAGCTG
StyI 1190 CCAAGG C^C[AT][AT]GG
1604 CCATGG
Tru9I 272 TTAA T^TAA
575 TTAA
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# 3 OR MORE CUT ENZYMES ( 72 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AvaII BanI BanII BbvI BcnI
BmyI BpmI BsaI BsaJI BsiYI
BslI BsmAI BsmFI Bsp1286I BsrI
BssKI BstF5I BstNI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraII
EaeI EcoO109I EcoRII FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI MaeIII
MboI MboII MflI MnlI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV PleI PstI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
TaqI TfiI TseI Tsp45I TspRI
TthHB8I XhoII