Restriction map for
pF1KSDA0544
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# NO CUT ENZYMES ( 116 enzymes) #
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AatII Acc65I AclI AflII AflIII
AgeI Alw44I AocI Aor51HI ApaLI
AscI AseI AsnI Asp700I Asp718I
AviII AvrII BamHI BanI BbeI
BbvCI BciVI BclI BfaI BfrI
BlnI BlpI Bpu1102I BsaBI BsgI
BsiEI BsiWI BsmBI BsmI Bsp1407I
BspDI BspHI BspLU11I BsrBI BsrDI
BsrGI BssHII BstAPI BstBI BstXI
Bsu36I CelII ClaI CpoI DrdI
DsaI EagI EclXI Eco47III Eco52I
Eco81I EcoRI EcoRV EcoT14I EcoT22I
FbaI FseI FspI HincII HindII
HindIII HpaI KasI KpnI KspI
MaeI MamI MfeI MluI MslI
MunI NarI NcoI NdeI NheI
NotI NruI NsiI NspI NspV
PacI PinAI PmeI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI SapI ScaI SexAI
SfiI SfoI SfuI SgrAI SnaBI
SpeI SphI SspBI SspI StuI
StyI SwaI TfiI XbaI XcmI
XmnI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AccI 442 GTATAC GT^[AC][GT]AC
AccIII 85 TCCGGA T^CCGGA
AcsI 127 AAATTC [GA]^AATT[CT]
AhdI 1261 GACGGCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 1039 CAGAGCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 127 AAATTC [GA]^AATT[CT]
AspEI 1261 GACGGCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbrPI 652 CACGTG CAC^GTG
BcgI 372 CGACGCCGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 755 AGATCT A^GATCT
BsaAI 652 CACGTG [CT]AC^GT[GA]
BsaWI 85 TCCGGA [AT]^CCGG[AT]
BseAI 85 TCCGGA T^CCGGA
BspEI 85 TCCGGA T^CCGGA
BsrFI 947 GCCGGC [GA]^CCGG[CT]
BssSI 232 CACGAG CACGAG(-5/-1)
Bst1107I 442 GTATAC GTA^TAC
BstEII 151 GGTTACC G^GT[ACGT]ACC
BstPI 151 GGTTACC G^GT[ACGT]ACC
BstZ17I 442 GTATAC GTA^TAC
Cfr10I 947 GCCGGC [GA]^CCGG[CT]
DraIII 1436 CACTGGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 1261 GACGGCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 151 GGTTACC G^GT[ACGT]ACC
HaeII 1179 GGCGCT [GA]GCGC^[CT]
MroI 85 TCCGGA T^CCGGA
MspA1I 1278 CAGCTG C[AC]G^C[GT]G
NaeI 947 GCCGGC GCC^GGC
NgoMIV 947 GCCGGC G^CCGGC
PaeR7I 1174 CTCGAG C^TCGAG
PmaCI 652 CACGTG CAC^GTG
PmlI 652 CACGTG CAC^GTG
PpuMI 1528 GGGACCC [GA]G^G[AT]CC[CT]
PvuII 1278 CAGCTG CAG^CTG
SmaI 341 CCCGGG CCC^GGG
SmlI 1174 CTCGAG C^T[CT][GA]AG
XhoI 1174 CTCGAG C^TCGAG
XmaCI 341 CCCGGG C^CCGGG
XmaI 341 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AcyI 373 GACGCC G[GA]^CG[CT]C
1423 GACGCC
AfaI 297 GTAC GT^AC
896 GTAC
ApaI 962 GGGCCC GGGCC^C
1624 GGGCCC
AspI 730 GACCGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1138 GACAGCGTC
BbsI 243 GAAGAC GAAGAC(2/6)
1374 GTCTTC
BpmI 358 CTGGAG CTGGAG(16/14)
1045 CTGGAG
BpuAI 243 GAAGAC GAAGAC(2/6)
1374 GTCTTC
BsaHI 373 GACGCC G[GA]^CG[CT]C
1423 GACGCC
BsaI 475 GAGACC GGTCTC(1/5)
787 GAGACC
DdeI 13 CTCAG C^T[ACGT]AG
1478 CTGAG
DraI 708 TTTAAA TTT^AAA
1072 TTTAAA
EarI 1390 GAAGAG CTCTTC(1/4)
1495 GAAGAG
EcoNI 744 CCTCCTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1266 CCTGTCCCAGG
Hin1I 373 GACGCC G[GA]^CG[CT]C
1423 GACGCC
Ksp632I 1390 GAAGAG CTCTTC(1/4)
1495 GAAGAG
MseI 709 TTAA T^TAA
1073 TTAA
PflFI 730 GACCGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1138 GACAGCGTC
RsaI 297 GTAC GT^AC
896 GTAC
SbfI 747 CCTGCAGG CCTGCA^GG
1557 CCTGCAGG
Sse8387I 747 CCTGCAGG CCTGCA^GG
1557 CCTGCAGG
Tru9I 709 TTAA T^TAA
1073 TTAA
Tsp509I 128 AATT ^AATT
723 AATT
TspRI 1436 CACTG CA[GC]TG[ACGT][ACGT]^
1579 CACTG
Tth111I 730 GACCGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1138 GACAGCGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 79 enzymes) #
#########################################
AccII AciI AluI AlwI AspHI
AvaI AvaII BalI BanII BbvI
BcnI BglI BmyI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrI BssKI
BstF5I BstNI BstUI BstYI Cac8I
CfoI Cfr13I DpnII DraII EaeI
EcoO109I EcoRII FauI Fnu4HI FokI
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII HphI ItaI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
PflMI PleI PstI Sau3AI Sau96I
ScrFI SfaNI SfcI TaqI TseI
Tsp45I TthHB8I Van91I XhoII