Restriction map for
pF1KSDA0550
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# NO CUT ENZYMES ( 86 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AflII AgeI Aor51HI ApaI AscI
Asp718I AvaI AviII AvrII BbeI
BbrPI BciVI BcnI BfrI BlnI
BsaAI BsaI BseAI BsiEI BsiWI
BsoBI BspDI BspEI BsrBI BssHII
BssSI BstBI BstUI ClaI CpoI
DraIII EagI EclXI Eco47III Eco52I
FseI FspI HaeII HpaI KasI
KpnI KspI MfeI MluI MroI
MunI MvnI NarI NciI NheI
NotI NruI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI PshAI
Psp1406I PvuI RsrII SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI Sse8387I SwaI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AcyI 3288 GGCGTC G[GA]^CG[CT]C
AhdI 622 GACGACCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1278 GTGCAC G^TGCAC
ApaLI 1278 GTGCAC G^TGCAC
AseI 2347 ATTAAT AT^TAAT
AsnI 2347 ATTAAT AT^TAAT
AspEI 622 GACGACCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 3883 GACATAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 3498 GGATCC G^GATCC
BanI 1627 GGCACC G^G[CT][GA]CC
BanII 3300 GAGCTC G[GA]GC[CT]^C
BbsI 1655 GTCTTC GAAGAC(2/6)
BbvCI 595 CCTCAGC CCTCAGC(-5/-2)
BcgI 3511 GCAGATTCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 2578 GCCATTTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 2310 AGATCT A^GATCT
BpuAI 1655 GTCTTC GAAGAC(2/6)
BsaBI 1697 GATGCATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 3288 GGCGTC G[GA]^CG[CT]C
BsaWI 2241 TCCGGT [AT]^CCGG[AT]
BseRI 1121 CTCCTC GAGGAG(10/8)
Bsp1407I 1354 TGTACA T^GTACA
BsrGI 1354 TGTACA T^GTACA
Bst1107I 576 GTATAC GTA^TAC
BstXI 4498 CCAGCAGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 576 GTATAC GTA^TAC
CfoI 2288 GCGC GCG^C
DraI 1888 TTTAAA TTT^AAA
DrdI 3333 GACGTGGATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 622 GACGACCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRV 4218 GATATC GAT^ATC
HhaI 2288 GCGC GCG^C
Hin1I 3288 GGCGTC G[GA]^CG[CT]C
HinP1I 2288 GCGC G^CGC
HindIII 2122 AAGCTT A^AGCTT
MamI 1697 GATGCATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI 1271 GCCGGC GCC^GGC
NgoMIV 1271 GCCGGC G^CCGGC
PflFI 3883 GACATAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1192 CCAGTTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI 2347 ATTAAT AT^TAAT
SacI 3300 GAGCTC GAGCT^C
SmlI 3896 CTCAAG C^T[CT][GA]AG
SphI 3540 GCATGC GCATG^C
SspBI 1354 TGTACA T^GTACA
StuI 2377 AGGCCT AGG^CCT
Tth111I 3883 GACATAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1192 CCAGTTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 1956 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AccI 576 GTATAC GT^[AC][GT]AC
3574 GTAGAC
AflIII 904 ACATGT A^C[GA][CT]GT
1078 ACATGT
AocI 826 CCTCAGG CC^T[ACGT]AGG
1543 CCTGAGG
BalI 2984 TGGCCA TGG^CCA
3353 TGGCCA
BclI 270 TGATCA T^GATCA
3027 TGATCA
BpmI 1580 CTCCAG CTGGAG(16/14)
2407 CTGGAG
BsmAI 2564 GTCTC GTCTC(1/5)
3291 GTCTC
BsmBI 2563 CGTCTC CGTCTC(1/5)
3290 CGTCTC
BspHI 810 TCATGA T^CATGA
3551 TCATGA
BspLU11I 904 ACATGT A^CATGT
1078 ACATGT
BsrFI 1271 GCCGGC [GA]^CCGG[CT]
3184 GCCGGT
BstEII 1052 GGTCACC G^GT[ACGT]ACC
1791 GGTGACC
BstPI 1052 GGTCACC G^GT[ACGT]ACC
1791 GGTGACC
Bsu36I 826 CCTCAGG CC^T[ACGT]AGG
1543 CCTGAGG
Cfr10I 1271 GCCGGC [GA]^CCGG[CT]
3184 GCCGGT
EaeI 2984 TGGCCA [CT]^GGCC[GA]
3353 TGGCCA
EarI 883 GAAGAG CTCTTC(1/4)
4293 GAAGAG
Eco81I 826 CCTCAGG CC^T[ACGT]AGG
1543 CCTGAGG
EcoNI 687 CCTGACCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1746 CCTTGCTAAGG
EcoO65I 1052 GGTCACC G^GT[ACGT]ACC
1791 GGTGACC
EcoRI 2079 GAATTC G^AATTC
4042 GAATTC
FauI 4387 CCCGC CCCGC(4/6)
4437 CCCGC
FbaI 270 TGATCA T^GATCA
3027 TGATCA
HincII 2185 GTTGAC GT[CT]^[GA]AC
3103 GTCAAC
HindII 2185 GTTGAC GT[CT]^[GA]AC
3103 GTCAAC
Ksp632I 883 GAAGAG CTCTTC(1/4)
4293 GAAGAG
MluNI 2984 TGGCCA TGG^CCA
3353 TGGCCA
MscI 2984 TGGCCA TGG^CCA
3353 TGGCCA
NdeI 4114 CATATG CA^TATG
4300 CATATG
PvuII 1286 CAGCTG CAG^CTG
4145 CAGCTG
RcaI 810 TCATGA T^CATGA
3551 TCATGA
SspI 1177 AATATT AAT^ATT
3985 AATATT
#########################################
# 3 OR MORE CUT ENZYMES ( 91 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI Asp700I AspHI AvaII
BbvI BfaI BlpI BmyI Bpu1102I
BsaJI BsgI BsiHKAI BsiYI BslI
BsmFI BsmI Bsp1286I BspMI BsrDI
BsrI BssKI BstAPI BstF5I BstNI
BstYI Cac8I CelII Cfr13I DdeI
DpnII DraII DsaI EcoO109I EcoRII
EcoT14I EcoT22I Fnu4HI FokI HaeIII
HapII HgaI HinfI HpaII HphI
ItaI MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MwoI NcoI
NdeII NlaIII NlaIV NsiI NspI
PleI PpuMI PstI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XcmI XhoII
XmnI