Restriction map for
pF1KSDA0557
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# NO CUT ENZYMES ( 110 enzymes) #
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AccIII AclI AcsI AflII AflIII
AgeI AhdI AocI ApoI AscI
AseI AsnI AspEI AspI AvrII
BbeI BbrPI BbvCI BcgI BciVI
BfrI BglI BglII BlnI BsaAI
BsaBI BsaI BsaWI BseAI BsiWI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrDI BsrGI BssHII BstBI
BstEII BstPI Bsu36I ClaI CpoI
DraI DrdI Eam1105I Eco81I EcoO65I
EcoRI EcoRV FseI HincII HindII
HindIII HpaI KasI KspI MaeIII
MamI MfeI MluI MroI MunI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NspI NspV PacI
PaeR7I PflFI PinAI PleI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacII SalI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
Tsp45I Tsp509I Tth111I XbaI XhoI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AatII 1008 GACGTC GACGT^C
Acc65I 824 GGTACC G^GTACC
AccI 691 GTATAC GT^[AC][GT]AC
Alw44I 1120 GTGCAC G^TGCAC
ApaLI 1120 GTGCAC G^TGCAC
Asp718I 824 GGTACC G^GTACC
AviII 248 TGCGCA TGC^GCA
BamHI 767 GGATCC G^GATCC
BbsI 941 GAAGAC GAAGAC(2/6)
BclI 912 TGATCA T^GATCA
BfaI 1066 CTAG C^TAG
BpuAI 941 GAAGAC GAAGAC(2/6)
BsmBI 118 GAGACG CGTCTC(1/5)
BsrFI 1407 GCCGGT [GA]^CCGG[CT]
BssSI 370 CTCGTG CACGAG(-5/-1)
Bst1107I 691 GTATAC GTA^TAC
BstAPI 1232 GCACCCAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 849 CCAGGGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 691 GTATAC GTA^TAC
Cfr10I 1407 GCCGGT [GA]^CCGG[CT]
DraIII 1233 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 238 CGGCCG C^GGCCG
EclXI 238 CGGCCG C^GGCCG
Eco52I 238 CGGCCG C^GGCCG
EcoNI 48 CCTGGAACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 717 ATGCAT ATGCA^T
FbaI 912 TGATCA T^GATCA
FspI 248 TGCGCA TGC^GCA
HinfI 524 GATTC G^A[ACGT]TC
KpnI 824 GGTACC GGTAC^C
MaeI 1066 CTAG C^TAG
MseI 469 TTAA T^TAA
MslI 1291 CACACAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI 954 CCATGG C^CATGG
NsiI 717 ATGCAT ATGCA^T
PstI 382 CTGCAG CTGCA^G
PvuII 550 CAGCTG CAG^CTG
SacI 1176 GAGCTC GAGCT^C
SfcI 382 CTGCAG C^T[GA][CT]AG
SmaI 191 CCCGGG CCC^GGG
StuI 907 AGGCCT AGG^CCT
TaqI 522 TCGA T^CGA
TfiI 524 GATTC G^A[AT]TC
Tru9I 469 TTAA T^TAA
TthHB8I 522 TCGA T^CGA
XcmI 174 CCAGGAGGTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 191 CCCGGG C^CCGGG
XmaI 191 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 21 enzymes) #
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AccII 331 CGCG CG^CG
741 CGCG
AcyI 627 GGCGTC G[GA]^CG[CT]C
1008 GACGTC
Aor51HI 1163 AGCGCT AGC^GCT
1247 AGCGCT
ApaI 187 GGGCCC GGGCC^C
573 GGGCCC
Asp700I 1161 GAAGCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1245 GAAGCGCTTC
BsaHI 627 GGCGTC G[GA]^CG[CT]C
1008 GACGTC
BsgI 1230 CTGCAC GTGCAG(16/14)
1354 CTGCAC
BsiEI 238 CGGCCG CG[GA][CT]^CG
1089 CGACCG
BsmAI 118 GAGAC GTCTC(1/5)
139 GAGAC
BstF5I 1191 CATCC GGATG(2/0)
801 GGATG
BstUI 331 CGCG CG^CG
741 CGCG
Eco47III 1163 AGCGCT AGC^GCT
1247 AGCGCT
FokI 1191 CATCC GGATG(9/13)
801 GGATG
Hin1I 627 GGCGTC G[GA]^CG[CT]C
1008 GACGTC
MaeII 683 ACGT A^CGT
1009 ACGT
MvnI 331 CGCG CG^CG
741 CGCG
PflMI 174 CCAGGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1404 CCAGCCGGTGG
SapI 153 GCTCTTC GCTCTTC(1/4)
162 GCTCTTC
TspRI 6 CACTG CA[GC]TG[ACGT][ACGT]^
1237 CAGTG
Van91I 174 CCAGGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1404 CCAGCCGGTGG
XmnI 1161 GAAGCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1245 GAAGCGCTTC
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# 3 OR MORE CUT ENZYMES ( 79 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AspHI AvaI AvaII BalI BanI
BanII BbvI BcnI BlpI BmyI
BpmI Bpu1102I BsaJI BseRI BsiHKAI
BsiYI BslI BsmFI BsoBI Bsp1286I
BspMI BsrBI BsrI BssKI BstNI
BstYI Cac8I CelII CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoO109I EcoRII EcoT14I FauI
Fnu4HI HaeII HaeIII HapII HgaI
HhaI HinP1I HpaII HphI ItaI
Ksp632I MboI MboII MflI MluNI
MnlI MscI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
PpuMI RsaI Sau3AI Sau96I ScrFI
SfaNI StyI TseI XhoII