Restriction map for
pF1KSDA0558
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# NO CUT ENZYMES ( 72 enzymes) #
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Acc65I AccIII AcsI AflII AgeI
Alw44I Aor51HI ApaLI ApoI AscI
AseI AsnI Asp718I BamHI BbrPI
BcgI BciVI BfrI BglII BsaBI
BseAI BsiWI BspDI BspEI BspLU11I
BssSI Bst1107I BstBI BstEII BstPI
BstZ17I ClaI CpoI DraI DrdI
Eco47III EcoO65I EcoRI EcoRV EcoT22I
FseI HindIII KpnI MamI MluI
MroI NdeI NotI NruI NsiI
NspI NspV PacI PaeR7I PinAI
PmaCI PmeI PmlI PshBI PvuI
RsrII SapI SfiI SfuI SgrAI
SnaBI SpeI SphI SspI StuI
SwaI XhoI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AccI 241 GTCGAC GT^[AC][GT]AC
AclI 1821 AACGTT AA^CGTT
AhdI 955 GACACGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 558 CCTAAGG CC^T[ACGT]AGG
AspEI 955 GACACGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 871 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 1049 TGCGCA TGC^GCA
AvrII 2472 CCTAGG C^CTAGG
BclI 1184 TGATCA T^GATCA
BglI 3387 GCCGCCCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 2472 CCTAGG C^CTAGG
BseRI 3373 CTCCTC GAGGAG(10/8)
BsiEI 3394 CGGCCG CG[GA][CT]^CG
BsmI 2064 GAATGC GAATGC(1/-1)
Bsp1407I 621 TGTACA T^GTACA
BsrBI 906 GAGCGG CCGCTC(-3/-3)
BsrDI 2034 CATTGC GCAATG(2/0)
BsrGI 621 TGTACA T^GTACA
BssHII 55 GCGCGC G^CGCGC
Bsu36I 558 CCTAAGG CC^T[ACGT]AGG
EagI 3394 CGGCCG C^GGCCG
Eam1105I 955 GACACGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 3394 CGGCCG C^GGCCG
Eco52I 3394 CGGCCG C^GGCCG
Eco81I 558 CCTAAGG CC^T[ACGT]AGG
FbaI 1184 TGATCA T^GATCA
FspI 1049 TGCGCA TGC^GCA
HpaI 2626 GTTAAC GTT^AAC
MfeI 1242 CAATTG C^AATTG
MunI 1242 CAATTG C^AATTG
NcoI 1415 CCATGG C^CATGG
NheI 2550 GCTAGC G^CTAGC
PflFI 871 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 785 CCACCCCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 933 GACATCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 1821 AACGTT AA^CGTT
SalI 241 GTCGAC G^TCGAC
SbfI 1971 CCTGCAGG CCTGCA^GG
SmaI 2846 CCCGGG CCC^GGG
Sse8387I 1971 CCTGCAGG CCTGCA^GG
SspBI 621 TGTACA T^GTACA
TfiI 2669 GATTC G^A[AT]TC
Tsp509I 1243 AATT ^AATT
Tth111I 871 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 785 CCACCCCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 1395 TCTAGA T^CTAGA
XmaCI 2846 CCCGGG C^CCGGG
XmaI 2846 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 22 enzymes) #
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ApaI 115 GGGCCC GGGCC^C
218 GGGCCC
BalI 1091 TGGCCA TGG^CCA
1246 TGGCCA
BbvCI 2815 CCTCAGC CCTCAGC(-5/-2)
2302 GCTGAGG
BlpI 502 GCTGAGC GC^T[ACGT]AGC
2466 GCTCAGC
Bpu1102I 502 GCTGAGC GC^T[ACGT]AGC
2466 GCTCAGC
BsaAI 1669 TACGTG [CT]AC^GT[GA]
2232 CACGTA
BsmBI 2998 GAGACG CGTCTC(1/5)
3199 GAGACG
BspHI 1187 TCATGA T^CATGA
3020 TCATGA
BstXI 866 CCAAAGACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1415 CCATGGTGCTGG
CelII 502 GCTGAGC GC^T[ACGT]AGC
2466 GCTCAGC
DraIII 786 CACCCCGTG CAC[ACGT][ACGT][ACGT]^GTG
1030 CACCTGGTG
EarI 402 GAAGAG CTCTTC(1/4)
1768 GAAGAG
Ksp632I 402 GAAGAG CTCTTC(1/4)
1768 GAAGAG
KspI 2975 CCGCGG CCGC^GG
3318 CCGCGG
MluNI 1091 TGGCCA TGG^CCA
1246 TGGCCA
MscI 1091 TGGCCA TGG^CCA
1246 TGGCCA
RcaI 1187 TCATGA T^CATGA
3020 TCATGA
SacII 2975 CCGCGG CCGC^GG
3318 CCGCGG
ScaI 2048 AGTACT AGT^ACT
3035 AGTACT
SexAI 1031 ACCTGGT A^CC[AT]GGT
2705 ACCAGGT
SmlI 1690 CTCAAG C^T[CT][GA]AG
3404 CTCAAG
XcmI 2738 CCAACCTGATGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3168 CCAGTGCGAGGCTGG
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# 3 OR MORE CUT ENZYMES ( 116 enzymes) #
#########################################
AatII AccII AciI AcyI AfaI
AflIII AluI AlwI AlwNI Asp700I
AspHI AvaI AvaII BanI BanII
BbeI BbsI BbvI BcnI BfaI
BmyI BpmI BpuAI BsaHI BsaI
BsaJI BsaWI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrFI BsrI BssKI BstAPI
BstF5I BstNI BstUI BstYI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HinfI
HpaII HphI ItaI KasI MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MvnI MwoI NaeI NarI
NciI NdeII NgoMIV NlaIII NlaIV
PleI PpuMI PstI PvuII RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI SfoI StyI TaqI Tru9I
TseI Tsp45I TspRI TthHB8I XhoII
XmnI