Restriction map for
pF1KSDA0573
##################################
# NO CUT ENZYMES ( 160 enzymes) #
##################################
AatII Acc65I AccII AciI AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI AscI Asp718I AspEI AspI
AvaI AviII AvrII BalI BamHI
BanI BbeI BbrPI BbvCI BciVI
BcnI BfrI BglI BglII BlnI
BlpI Bpu1102I BsaAI BsaHI BsaI
BseRI BsiEI BsiWI BsiYI BslI
BsmBI BsoBI Bsp1407I BspDI BspHI
BspLU11I BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI
BstEII BstPI BstUI BstXI BstYI
Bsu36I CelII CfoI Cfr10I ClaI
CpoI DraII DrdI DsaI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRV
EcoT14I FauI FseI FspI HaeII
HaeIII HgaI HhaI Hin1I HinP1I
HincII HindII HpaI KasI KpnI
KspI MaeII MflI MluI MluNI
MscI MspA1I MvnI NaeI NarI
NciI NcoI NgoMIV NheI NlaIV
NotI NruI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI Psp1406I PstI PvuI
PvuII RcaI RsrII SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI StuI
StyI SwaI Tth111I Van91I XbaI
XcmI XhoI XhoII XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 56 enzymes) #
###################################
AccI 1188 GTATAC GT^[AC][GT]AC
AccIII 654 TCCGGA T^CCGGA
AlwI 1203 GGATC GGATC(4/5)
Asp700I 229 GAAGAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 1152 GAGCTC G[AT]GC[AT]^C
AvaII 1087 GGACC G^G[AT]CC
BanII 1152 GAGCTC G[GA]GC[CT]^C
BbsI 13 GTCTTC GAAGAC(2/6)
BbvI 473 GCAGC GCAGC(8/12)
BcgI 545 CGAATATTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 276 TGATCA T^GATCA
BfaI 1168 CTAG C^TAG
BmyI 1152 GAGCTC G[AGT]GC[ACT]^C
BpmI 941 CTCCAG CTGGAG(16/14)
BpuAI 13 GTCTTC GAAGAC(2/6)
BsaBI 271 GATTGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 654 TCCGGA T^CCGGA
BsiHKAI 1152 GAGCTC G[AT]GC[AT]^C
BsmAI 998 GAGAC GTCTC(1/5)
BsmFI 443 GGGAC GGGAC(10/14)
BsmI 645 GCATTC GAATGC(1/-1)
Bsp1286I 1152 GAGCTC G[AGT]GC[ACT]^C
BspEI 654 TCCGGA T^CCGGA
Bst1107I 1188 GTATAC GTA^TAC
BstZ17I 1188 GTATAC GTA^TAC
Cfr13I 1087 GGACC G^G[ACGT]CC
DraI 126 TTTAAA TTT^AAA
DraIII 1172 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoRI 1075 GAATTC G^AATTC
EcoT22I 1 ATGCAT ATGCA^T
FbaI 276 TGATCA T^GATCA
Fnu4HI 473 GCAGC GC^[ACGT]GC
HindIII 209 AAGCTT A^AGCTT
HphI 458 TCACC GGTGA(8/7)
ItaI 473 GCAGC GC^[ACGT]GC
MaeI 1168 CTAG C^TAG
MamI 271 GATTGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 702 CAATTG C^AATTG
MroI 654 TCCGGA T^CCGGA
MslI 982 CATATGTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 702 CAATTG C^AATTG
MwoI 136 GCTGATGTTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NdeI 982 CATATG CA^TATG
NsiI 1 ATGCAT ATGCA^T
NspI 891 ACATGC [GA]CATG^[CT]
PleI 511 GACTC GAGTC(4/5)
PshAI 166 GACTGGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 1152 GAGCTC GAGCT^C
Sau96I 1087 GGACC G^G[ACGT]CC
SfcI 522 CTATAG C^T[GA][CT]AG
SmlI 1030 CTCAAG C^T[CT][GA]AG
TaqI 1206 TCGA T^CGA
TfiI 708 GATTC G^A[AT]TC
TseI 473 GCAGC G^C[AT]GC
TthHB8I 1206 TCGA T^CGA
XmnI 229 GAAGAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AfaI 666 GTAC GT^AC
875 GTAC
AseI 858 ATTAAT AT^TAAT
1014 ATTAAT
AsnI 858 ATTAAT AT^TAAT
1014 ATTAAT
BsaJI 640 CCAGGG C^C[ACGT][ACGT]GG
1110 CCCAGG
BsaWI 600 ACCGGA [AT]^CCGG[AT]
654 TCCGGA
BsgI 925 CTGCAC GTGCAG(16/14)
1066 CTGCAC
BsrI 167 ACTGG ACTGG(1/-1)
1175 CCAGT
Cac8I 1119 GCAAGC GC[ACGT]^[ACGT]GC
1135 GCAAGC
EarI 920 CTCTTC CTCTTC(1/4)
357 GAAGAG
HapII 601 CCGG C^CGG
655 CCGG
HinfI 511 GACTC G^A[ACGT]TC
708 GATTC
HpaII 601 CCGG C^CGG
655 CCGG
Ksp632I 920 CTCTTC CTCTTC(1/4)
357 GAAGAG
MspI 601 CCGG C^CGG
655 CCGG
PshBI 858 ATTAAT AT^TAAT
1014 ATTAAT
RsaI 666 GTAC GT^AC
875 GTAC
SspI 547 AATATT AAT^ATT
831 AATATT
TspRI 383 CAGTG CA[GC]TG[ACGT][ACGT]^
1176 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 24 enzymes) #
#########################################
AcsI AluI ApoI BssKI BstF5I
BstNI DdeI DpnII EcoRII FokI
MaeIII MboI MboII MnlI MseI
MvaI NdeII NlaIII Sau3AI ScrFI
SfaNI Tru9I Tsp45I Tsp509I