Restriction map for
pF1KSDA0578
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# NO CUT ENZYMES ( 57 enzymes) #
##################################
AatII AccI AclI AflII AgeI
Alw44I AocI ApaLI AscI AvrII
BamHI BbrPI BfrI BglI BglII
BlnI BsaI BsiWI BspHI BspLU11I
Bst1107I BstBI BstZ17I Bsu36I CpoI
Eco81I FseI HindIII MfeI MluI
MunI NaeI NgoMIV NheI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI Psp1406I PvuI RcaI RsrII
SalI SfiI SfuI SgrAI SmaI
SnaBI SphI SspI StuI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 47 enzymes) #
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Acc65I 1735 GGTACC G^GTACC
Aor51HI 3680 AGCGCT AGC^GCT
ApaI 3220 GGGCCC GGGCC^C
Asp700I 3115 GAAGGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1735 GGTACC G^GTACC
AspI 3746 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 350 TGCGCA TGC^GCA
BcgI 3912 CGAAAACGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 4215 GTATCC GTATCC(6/5)
BclI 2487 TGATCA T^GATCA
BsaBI 1362 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI 2046 GTGCAG GTGCAG(16/14)
BsiEI 258 CGGCCG CG[GA][CT]^CG
BsmBI 1721 GAGACG CGTCTC(1/5)
BspDI 1921 ATCGAT AT^CGAT
BsrBI 526 GAGCGG CCGCTC(-3/-3)
BssHII 486 GCGCGC G^CGCGC
BstEII 1256 GGTCACC G^GT[ACGT]ACC
BstPI 1256 GGTCACC G^GT[ACGT]ACC
ClaI 1921 ATCGAT AT^CGAT
EagI 258 CGGCCG C^GGCCG
EclXI 258 CGGCCG C^GGCCG
Eco47III 3680 AGCGCT AGC^GCT
Eco52I 258 CGGCCG C^GGCCG
EcoNI 515 CCTCGGTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1256 GGTCACC G^GT[ACGT]ACC
EcoRI 3734 GAATTC G^AATTC
FbaI 2487 TGATCA T^GATCA
FspI 350 TGCGCA TGC^GCA
KpnI 1735 GGTACC GGTAC^C
MamI 1362 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NdeI 2228 CATATG CA^TATG
NotI 257 GCGGCCGC GC^GGCCGC
NruI 736 TCGCGA TCG^CGA
PflFI 3746 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1371 CCATGGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 4363 GACGAGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 4176 GAGCTC GAGCT^C
SapI 381 GCTCTTC GCTCTTC(1/4)
SbfI 3688 CCTGCAGG CCTGCA^GG
ScaI 1966 AGTACT AGT^ACT
SexAI 392 ACCAGGT A^CC[AT]GGT
SpeI 1059 ACTAGT A^CTAGT
Sse8387I 3688 CCTGCAGG CCTGCA^GG
Tth111I 3746 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1371 CCATGGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 3115 GAAGGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 37 enzymes) #
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AccIII 2083 TCCGGA T^CCGGA
3162 TCCGGA
AcsI 2159 AAATTC [GA]^AATT[CT]
3734 GAATTC
AhdI 1639 GACATGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1723 GACGGACGGTC
ApoI 2159 AAATTC [GA]^AATT[CT]
3734 GAATTC
AspEI 1639 GACATGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1723 GACGGACGGTC
AvaI 2359 CCCGAG C^[CT]CG[GA]G
4193 CCCGAG
BbeI 103 GGCGCC GGCGC^C
334 GGCGCC
BbsI 772 GAAGAC GAAGAC(2/6)
1852 GTCTTC
BbvCI 4127 CCTCAGC CCTCAGC(-5/-2)
2191 GCTGAGG
BpuAI 772 GAAGAC GAAGAC(2/6)
1852 GTCTTC
BsaWI 2083 TCCGGA [AT]^CCGG[AT]
3162 TCCGGA
BseAI 2083 TCCGGA T^CCGGA
3162 TCCGGA
BsmI 1464 GAATGC GAATGC(1/-1)
2234 GCATTC
BsoBI 2359 CCCGAG C^[CT]CG[GA]G
4193 CCCGAG
Bsp1407I 3102 TGTACA T^GTACA
4322 TGTACA
BspEI 2083 TCCGGA T^CCGGA
3162 TCCGGA
BsrDI 3332 GCAATG GCAATG(2/0)
3641 GCAATG
BsrFI 321 GCCGGT [GA]^CCGG[CT]
742 ACCGGC
BsrGI 3102 TGTACA T^GTACA
4322 TGTACA
Cfr10I 321 GCCGGT [GA]^CCGG[CT]
742 ACCGGC
DrdI 556 GACGTGAGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3978 GACAACTGAGTC
Eam1105I 1639 GACATGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1723 GACGGACGGTC
EarI 382 CTCTTC CTCTTC(1/4)
4067 GAAGAG
EcoRV 1939 GATATC GAT^ATC
2952 GATATC
EcoT22I 2179 ATGCAT ATGCA^T
2755 ATGCAT
HpaI 325 GTTAAC GTT^AAC
2442 GTTAAC
KasI 103 GGCGCC G^GCGCC
334 GGCGCC
Ksp632I 382 CTCTTC CTCTTC(1/4)
4067 GAAGAG
MroI 2083 TCCGGA T^CCGGA
3162 TCCGGA
NarI 103 GGCGCC GG^CGCC
334 GGCGCC
NcoI 1371 CCATGG C^CATGG
1536 CCATGG
NsiI 2179 ATGCAT ATGCA^T
2755 ATGCAT
PvuII 3291 CAGCTG CAG^CTG
3663 CAGCTG
SfoI 103 GGCGCC GGC^GCC
334 GGCGCC
SspBI 3102 TGTACA T^GTACA
4322 TGTACA
SwaI 1386 ATTTAAAT ATTT^AAAT
3146 ATTTAAAT
XbaI 2254 TCTAGA T^CTAGA
2319 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 117 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI AlwI AlwNI AseI AsnI
AspHI AvaII BalI BanI BanII
BbvI BcnI BfaI BlpI BmyI
BpmI Bpu1102I BsaAI BsaHI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BspMI BsrI BssKI
BssSI BstAPI BstF5I BstNI BstUI
BstXI BstYI Cac8I CelII CfoI
Cfr13I DdeI DpnII DraI DraII
DraIII DsaI EaeI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HinfI HpaII
HphI ItaI KspI MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NspI PleI
PpuMI PshBI PstI RsaI SacII
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XcmI XhoII