Restriction map for
pF1KSDA0588
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# NO CUT ENZYMES ( 84 enzymes) #
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AatII Acc65I AclI AcsI AflII
AgeI AhdI Alw44I Aor51HI ApaI
ApaLI ApoI AscI Asp718I AspEI
AvrII BalI BbeI BciVI BfrI
BglI BglII BlnI BsaBI BsiEI
BsiWI BsmI Bsp1407I BspDI BspHI
BspLU11I BsrGI Bst1107I BstBI BstZ17I
ClaI CpoI EagI Eam1105I EclXI
Eco47III Eco52I EcoRI EcoT22I FseI
HindIII HpaI KasI KpnI MamI
MfeI MluI MluNI MscI MunI
NarI NdeI NheI NotI NruI
NsiI NspV PacI PinAI PmeI
PshAI Psp1406I PvuI RcaI RsrII
SalI SbfI ScaI SfiI SfoI
SfuI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI XcmI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AccI 2683 GTCTAC GT^[AC][GT]AC
AlwNI 437 CAGCCACTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1357 CCTCAGG CC^T[ACGT]AGG
AseI 1092 ATTAAT AT^TAAT
AsnI 1092 ATTAAT AT^TAAT
Asp700I 1237 GAACAGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 1219 GACATAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 474 GGATCC G^GATCC
BbrPI 2659 CACGTG CAC^GTG
BbvCI 1623 CCTCAGC CCTCAGC(-5/-2)
BcgI 1495 GCAAGCCTATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 1001 TGATCA T^GATCA
BspMI 2749 GCAGGT ACCTGC(4/8)
BsrBI 1737 CCGCTC CCGCTC(-3/-3)
BssHII 676 GCGCGC G^CGCGC
BstAPI 2655 GCAGCACGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1761 GGTGACC G^GT[ACGT]ACC
BstPI 1761 GGTGACC G^GT[ACGT]ACC
Bsu36I 1357 CCTCAGG CC^T[ACGT]AGG
DraIII 2215 CACTTTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 1357 CCTCAGG CC^T[ACGT]AGG
EcoO65I 1761 GGTGACC G^GT[ACGT]ACC
EcoRV 481 GATATC GAT^ATC
FbaI 1001 TGATCA T^GATCA
HincII 790 GTCAAC GT[CT]^[GA]AC
HindII 790 GTCAAC GT[CT]^[GA]AC
KspI 2093 CCGCGG CCGC^GG
NcoI 2615 CCATGG C^CATGG
NspI 2330 ACATGC [GA]CATG^[CT]
PaeR7I 2032 CTCGAG C^TCGAG
PflFI 1219 GACATAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 2659 CACGTG CAC^GTG
PmlI 2659 CACGTG CAC^GTG
PpuMI 149 GGGACCT [GA]G^G[AT]CC[CT]
PshBI 1092 ATTAAT AT^TAAT
SacII 2093 CCGCGG CCGC^GG
SapI 109 GAAGAGC GCTCTTC(1/4)
SgrAI 2204 CGCCGGCG C[GA]^CCGG[CT]G
SmaI 167 CCCGGG CCC^GGG
StuI 1361 AGGCCT AGG^CCT
Tth111I 1219 GACATAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 315 TCTAGA T^CTAGA
XhoI 2032 CTCGAG C^TCGAG
XmaCI 167 CCCGGG C^CCGGG
XmaI 167 CCCGGG C^CCGGG
XmnI 1237 GAACAGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 26 enzymes) #
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AcyI 2208 GGCGTC G[GA]^CG[CT]C
2559 GGCGTC
AflIII 1511 ACGTGT A^C[GA][CT]GT
1634 ACGTGT
AviII 662 TGCGCA TGC^GCA
1877 TGCGCA
BbsI 1351 GTCTTC GAAGAC(2/6)
2107 GTCTTC
BpuAI 1351 GTCTTC GAAGAC(2/6)
2107 GTCTTC
BsaAI 1510 TACGTG [CT]AC^GT[GA]
2659 CACGTG
BsaHI 2208 GGCGTC G[GA]^CG[CT]C
2559 GGCGTC
BsmBI 1413 CGTCTC CGTCTC(1/5)
1904 GAGACG
BssSI 925 CACGAG CACGAG(-5/-1)
2263 CACGAG
BstYI 474 GGATCC [GA]^GATC[CT]
1682 AGATCC
DraI 1104 TTTAAA TTT^AAA
1174 TTTAAA
DraII 149 GGGACCT [GA]G^G[ACGT]CC[CT]
2741 AGGCCCC
DrdI 808 GACGAAGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2329 GACATGCTCGTC
EcoNI 805 CCTGACGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2169 CCTGCTGCAGG
EcoO109I 149 GGGACCT [GA]G^G[ACGT]CC[CT]
2741 AGGCCCC
FspI 662 TGCGCA TGC^GCA
1877 TGCGCA
Hin1I 2208 GGCGTC G[GA]^CG[CT]C
2559 GGCGTC
MflI 474 GGATCC [GA]^GATC[CT]
1682 AGATCC
NaeI 2205 GCCGGC GCC^GGC
2608 GCCGGC
NgoMIV 2205 GCCGGC G^CCGGC
2608 GCCGGC
PflMI 2007 CCAAGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2746 CCAGCAGGTGG
PvuII 778 CAGCTG CAG^CTG
2720 CAGCTG
TaqI 1559 TCGA T^CGA
2033 TCGA
TthHB8I 1559 TCGA T^CGA
2033 TCGA
Van91I 2007 CCAAGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2746 CCAGCAGGTGG
XhoII 474 GGATCC [GA]^GATC[CT]
1682 AGATCC
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# 3 OR MORE CUT ENZYMES ( 102 enzymes) #
#########################################
AccII AccIII AciI AfaI AluI
AlwI AspHI AvaI AvaII BanI
BanII BbvI BcnI BfaI BlpI
BmyI BpmI Bpu1102I BsaI BsaJI
BsaWI BseAI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspEI BsrDI BsrFI BsrI
BssKI BstF5I BstNI BstUI BstXI
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DsaI EaeI EarI
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MnlI MroI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV PleI
PstI RsaI SacI Sau3AI Sau96I
ScrFI SexAI SfaNI SfcI SmlI
StyI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI