Restriction map for
pF1KSDA0601
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# NO CUT ENZYMES ( 104 enzymes) #
##################################
AatII Acc65I AclI AflIII AhdI
Alw44I AlwNI Aor51HI ApaLI AscI
AseI AsnI Asp718I AspEI AspI
AviII AvrII BbeI BbrPI BbsI
BcgI BciVI BclI BglII BlnI
BpmI BpuAI BsaAI BseRI BsiEI
BsiWI BsmBI BsmI Bsp1407I BspDI
BspLU11I BspMI BsrDI BsrGI BssHII
BstAPI BstBI BstEII BstPI BstXI
ClaI CpoI DraI EagI Eam1105I
EclXI Eco47III Eco52I EcoO65I EcoRI
EcoRV EcoT22I FbaI FspI HaeII
HincII HindII HpaI KasI KpnI
MaeII MfeI MluI MslI MunI
NarI NdeI NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI RsrII SalI
SbfI ScaI SfoI SfuI SgrAI
SnaBI SphI Sse8387I SspBI SspI
SwaI Tth111I XbaI XhoI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AccI 2499 GTATAC GT^[AC][GT]AC
AccIII 312 TCCGGA T^CCGGA
AcyI 320 GGCGTC G[GA]^CG[CT]C
AflII 1645 CTTAAG C^TTAAG
AgeI 1148 ACCGGT A^CCGGT
ApaI 135 GGGCCC GGGCC^C
Asp700I 1516 GAAAAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BamHI 2013 GGATCC G^GATCC
BanI 2362 GGTGCC G^G[CT][GA]CC
BbvCI 2221 CCTCAGC CCTCAGC(-5/-2)
BfrI 1645 CTTAAG C^TTAAG
BlpI 2329 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 2329 GCTCAGC GC^T[ACGT]AGC
BsaBI 1552 GATGTATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 320 GGCGTC G[GA]^CG[CT]C
BsaI 861 GGTCTC GGTCTC(1/5)
BseAI 312 TCCGGA T^CCGGA
BspEI 312 TCCGGA T^CCGGA
BspHI 552 TCATGA T^CATGA
BsrBI 1821 CCGCTC CCGCTC(-3/-3)
Bst1107I 2499 GTATAC GTA^TAC
BstZ17I 2499 GTATAC GTA^TAC
CelII 2329 GCTCAGC GC^T[ACGT]AGC
DraIII 2522 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1189 GACTTCAATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FseI 252 GGCCGGCC GGCCGG^CC
Hin1I 320 GGCGTC G[GA]^CG[CT]C
HphI 2065 GGTGA GGTGA(8/7)
MamI 1552 GATGTATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 312 TCCGGA T^CCGGA
PinAI 1148 ACCGGT A^CCGGT
PvuII 682 CAGCTG CAG^CTG
RcaI 552 TCATGA T^CATGA
SacI 2068 GAGCTC GAGCT^C
SapI 2070 GCTCTTC GCTCTTC(1/4)
SexAI 2523 ACCAGGT A^CC[AT]GGT
SfiI 127 GGCCTGTCGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 211 CCCGGG CCC^GGG
SpeI 2115 ACTAGT A^CTAGT
StuI 1740 AGGCCT AGG^CCT
XmaCI 211 CCCGGG C^CCGGG
XmaI 211 CCCGGG C^CCGGG
XmnI 1516 GAAAAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AcsI 1347 AAATTT [GA]^AATT[CT]
1609 GAATTT
AocI 247 CCTCAGG CC^T[ACGT]AGG
478 CCTGAGG
ApoI 1347 AAATTT [GA]^AATT[CT]
1609 GAATTT
AspHI 2068 GAGCTC G[AT]GC[AT]^C
2438 GTGCTC
AvaI 211 CCCGGG C^[CT]CG[GA]G
2270 CTCGGG
BglI 25 GCCGCGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
128 GCCTGTCGGGC
BsgI 2534 CTGCAC GTGCAG(16/14)
2213 GTGCAG
BsiHKAI 2068 GAGCTC G[AT]GC[AT]^C
2438 GTGCTC
BsoBI 211 CCCGGG C^[CT]CG[GA]G
2270 CTCGGG
BssSI 1827 CACGAG CACGAG(-5/-1)
1713 CTCGTG
BstYI 1455 GGATCT [GA]^GATC[CT]
2013 GGATCC
Bsu36I 247 CCTCAGG CC^T[ACGT]AGG
478 CCTGAGG
DraII 215 GGGGCCT [GA]G^G[ACGT]CC[CT]
243 AGGGCCT
Eco81I 247 CCTCAGG CC^T[ACGT]AGG
478 CCTGAGG
EcoO109I 215 GGGGCCT [GA]G^G[ACGT]CC[CT]
243 AGGGCCT
HindIII 375 AAGCTT A^AGCTT
448 AAGCTT
MflI 1455 GGATCT [GA]^GATC[CT]
2013 GGATCC
NaeI 253 GCCGGC GCC^GGC
332 GCCGGC
NcoI 287 CCATGG C^CATGG
992 CCATGG
NgoMIV 253 GCCGGC G^CCGGC
332 GCCGGC
NspI 1263 GCATGT [GA]CATG^[CT]
2037 GCATGT
TaqI 882 TCGA T^CGA
2353 TCGA
Tsp45I 910 GTCAC ^GT[GC]AC
2156 GTGAC
TthHB8I 882 TCGA T^CGA
2353 TCGA
XcmI 926 CCAGGGATCGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2228 CCAAAGAAACTGTGG
XhoII 1455 GGATCT [GA]^GATC[CT]
2013 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 85 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AvaII BalI BanII BbvI BcnI
BfaI BmyI BsaJI BsaWI BsiYI
BslI BsmAI BsmFI Bsp1286I BsrFI
BsrI BssKI BstF5I BstNI BstUI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DsaI EaeI EarI EcoNI
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII ItaI Ksp632I KspI
MaeI MaeIII MboI MboII MluNI
MnlI MscI MseI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV PflMI PleI PstI
RsaI SacII Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TfiI
Tru9I TseI Tsp509I TspRI Van91I