Restriction map for
pF1KSDA0608
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# NO CUT ENZYMES ( 96 enzymes) #
##################################
AatII Acc65I AccI AclI AflII
AgeI AhdI Alw44I AocI Aor51HI
ApaI ApaLI Asp718I AspEI AspI
AviII AvrII BamHI BbrPI BcgI
BciVI BclI BfrI BglII BlnI
BlpI Bpu1102I BsaAI BsaBI BsaI
BsgI BsiWI BsmI Bsp1407I BspDI
BspLU11I BsrGI BssSI Bst1107I BstBI
BstEII BstPI BstZ17I Bsu36I CelII
ClaI CpoI DraI DraIII DrdI
Eam1105I Eco47III Eco81I EcoNI EcoO65I
EcoRI EcoT22I FbaI FseI FspI
HincII HindII HpaI KpnI MamI
MluI NcoI NdeI NheI NotI
NsiI NspV PaeR7I PflFI PinAI
PmaCI PmeI PmlI Psp1406I RsrII
SacI SalI SapI SbfI SexAI
SfiI SfuI SnaBI SpeI SphI
Sse8387I SspBI SwaI Tth111I XbaI
XhoI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AccIII 9 TCCGGA T^CCGGA
AflIII 806 ACACGT A^C[GA][CT]GT
AlwNI 654 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
AscI 718 GGCGCGCC GG^CGCGCC
AseI 1617 ATTAAT AT^TAAT
AsnI 1617 ATTAAT AT^TAAT
AspHI 1001 GTGCTC G[AT]GC[AT]^C
BalI 258 TGGCCA TGG^CCA
BbsI 1122 GTCTTC GAAGAC(2/6)
BbvCI 1258 GCTGAGG CCTCAGC(-5/-2)
BpuAI 1122 GTCTTC GAAGAC(2/6)
BsaWI 9 TCCGGA [AT]^CCGG[AT]
BseAI 9 TCCGGA T^CCGGA
BsiHKAI 1001 GTGCTC G[AT]GC[AT]^C
BsmBI 169 GAGACG CGTCTC(1/5)
BspEI 9 TCCGGA T^CCGGA
BspMI 845 ACCTGC ACCTGC(4/8)
BsrBI 247 CCGCTC CCGCTC(-3/-3)
BstAPI 1428 GCAGGGATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 1205 CCAAATCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 1982 GGATCT [GA]^GATC[CT]
EcoRV 2167 GATATC GAT^ATC
EcoT14I 1746 CCAAGG C^C[AT][AT]GG
HindIII 1220 AAGCTT A^AGCTT
MfeI 1380 CAATTG C^AATTG
MflI 1982 GGATCT [GA]^GATC[CT]
MluNI 258 TGGCCA TGG^CCA
MroI 9 TCCGGA T^CCGGA
MscI 258 TGGCCA TGG^CCA
MunI 1380 CAATTG C^AATTG
NruI 420 TCGCGA TCG^CGA
NspI 1717 ACATGC [GA]CATG^[CT]
PacI 1618 TTAATTAA TTAAT^TAA
PflMI 2225 CCAAAAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 2235 GACTCAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 1617 ATTAAT AT^TAAT
PvuI 295 CGATCG CGAT^CG
ScaI 1770 AGTACT AGT^ACT
SmaI 911 CCCGGG CCC^GGG
StuI 1726 AGGCCT AGG^CCT
StyI 1746 CCAAGG C^C[AT][AT]GG
Tsp45I 1229 GTCAC ^GT[GC]AC
Van91I 2225 CCAAAAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 996 CCATAGTGCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 1982 GGATCT [GA]^GATC[CT]
XmaCI 911 CCCGGG C^CCGGG
XmaI 911 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 21 enzymes) #
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Asp700I 1542 GAAAAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2104 GAAGATATTC
BbeI 268 GGCGCC GGCGC^C
697 GGCGCC
BpmI 223 CTGGAG CTGGAG(16/14)
502 CTGGAG
BsmAI 169 GAGAC GTCTC(1/5)
2276 GAGAC
BspHI 1307 TCATGA T^CATGA
1683 TCATGA
BsrDI 1761 CATTGC GCAATG(2/0)
1348 GCAATG
KasI 268 GGCGCC G^GCGCC
697 GGCGCC
MaeII 808 ACGT A^CGT
1227 ACGT
MaeIII 197 GTTAC ^GT[ACGT]AC
1229 GTCAC
MslI 1712 CATACACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2205 CATTCAGATG
NaeI 265 GCCGGC GCC^GGC
454 GCCGGC
NarI 268 GGCGCC GG^CGCC
697 GGCGCC
NgoMIV 265 GCCGGC G^CCGGC
454 GCCGGC
PstI 660 CTGCAG CTGCA^G
2116 CTGCAG
PvuII 220 CAGCTG CAG^CTG
657 CAGCTG
RcaI 1307 TCATGA T^CATGA
1683 TCATGA
SfoI 268 GGCGCC GGC^GCC
697 GGCGCC
SgrAI 453 CGCCGGCG C[GA]^CCGG[CT]G
863 CGCCGGTG
SspI 1336 AATATT AAT^ATT
1880 AATATT
TspRI 1164 CACTG CA[GC]TG[ACGT][ACGT]^
1551 CAGTG
XmnI 1542 GAAAAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2104 GAAGATATTC
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# 3 OR MORE CUT ENZYMES ( 94 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI ApoI AvaI AvaII
BanI BanII BbvI BcnI BfaI
BglI BmyI BsaHI BsaJI BseRI
BsiEI BsiYI BslI BsmFI BsoBI
Bsp1286I BsrFI BsrI BssHII BssKI
BstF5I BstNI BstUI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EagI EarI EclXI
Eco52I EcoO109I EcoRII FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI Ksp632I KspI MaeI
MboI MboII MnlI MseI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV PleI PpuMI
RsaI SacII Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI TaqI TfiI
Tru9I TseI Tsp509I TthHB8I