Restriction map for
pF1KSDA0613
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# NO CUT ENZYMES ( 108 enzymes) #
##################################
AatII Acc65I AccIII AflII AflIII
AgeI AhdI Aor51HI AscI AseI
AsnI Asp700I Asp718I AspEI AvrII
BamHI BbeI BbrPI BbvCI BcgI
BciVI BclI BfaI BfrI BglI
BglII BlnI BsaAI BsaBI BsaWI
BseAI BsiEI BsiWI BsmBI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrDI BsrGI BssHII Bst1107I BstBI
BstYI BstZ17I ClaI CpoI DraI
DrdI EagI Eam1105I EclXI Eco47III
Eco52I EcoNI EcoRV FbaI FseI
HindIII HpaI KasI KpnI MaeI
MamI MfeI MflI MluI MroI
MslI MunI NaeI NarI NdeI
NgoMIV NheI NotI NruI NspV
PacI PflMI PinAI PmaCI PmeI
PmlI PshAI PshBI PvuI RcaI
RsrII SalI SbfI ScaI SexAI
SfiI SfoI SfuI SnaBI SpeI
Sse8387I SspBI SspI SwaI Van91I
XbaI XhoII XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AccII 491 CGCG CG^CG
AclI 1783 AACGTT AA^CGTT
AcsI 853 GAATTC [GA]^AATT[CT]
AcyI 160 GGCGTC G[GA]^CG[CT]C
Alw44I 921 GTGCAC G^TGCAC
ApaLI 921 GTGCAC G^TGCAC
ApoI 853 GAATTC [GA]^AATT[CT]
AspI 157 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 2089 TGCGCA TGC^GCA
BbsI 1951 GAAGAC GAAGAC(2/6)
BpuAI 1951 GAAGAC GAAGAC(2/6)
BsaHI 160 GGCGTC G[GA]^CG[CT]C
BsaI 613 GAGACC GGTCTC(1/5)
BseRI 456 CTCCTC GAGGAG(10/8)
BsrBI 326 CCGCTC CCGCTC(-3/-3)
BssSI 142 CTCGTG CACGAG(-5/-1)
BstEII 136 GGTGACC G^GT[ACGT]ACC
BstPI 136 GGTGACC G^GT[ACGT]ACC
BstUI 491 CGCG CG^CG
BstXI 1844 CCAAAATTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraIII 1998 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoO65I 136 GGTGACC G^GT[ACGT]ACC
EcoRI 853 GAATTC G^AATTC
EcoT22I 1864 ATGCAT ATGCA^T
FspI 2089 TGCGCA TGC^GCA
HaeII 721 AGCGCC [GA]GCGC^[CT]
Hin1I 160 GGCGTC G[GA]^CG[CT]C
HincII 163 GTCAAC GT[CT]^[GA]AC
HindII 163 GTCAAC GT[CT]^[GA]AC
KspI 490 CCGCGG CCGC^GG
MaeII 1784 ACGT A^CGT
MvnI 491 CGCG CG^CG
NsiI 1864 ATGCAT ATGCA^T
PaeR7I 564 CTCGAG C^TCGAG
PflFI 157 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
Psp1406I 1783 AACGTT AA^CGTT
PvuII 1013 CAGCTG CAG^CTG
SacI 409 GAGCTC GAGCT^C
SacII 490 CCGCGG CCGC^GG
SapI 1771 GAAGAGC GCTCTTC(1/4)
SgrAI 914 CGCCGGTG C[GA]^CCGG[CT]G
Tth111I 157 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 176 CCATGACCCACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 564 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 23 enzymes) #
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AfaI 603 GTAC GT^AC
639 GTAC
AlwI 306 GATCC GGATC(4/5)
92 GGATC
BalI 149 TGGCCA TGG^CCA
1007 TGGCCA
BlpI 126 GCTCAGC GC^T[ACGT]AGC
1615 GCTGAGC
BpmI 281 CTCCAG CTGGAG(16/14)
2110 CTGGAG
Bpu1102I 126 GCTCAGC GC^T[ACGT]AGC
1615 GCTGAGC
BsgI 1172 CTGCAC GTGCAG(16/14)
1412 CTGCAC
BsrFI 300 GCCGGT [GA]^CCGG[CT]
915 GCCGGT
BstAPI 1533 GCAGACCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1997 GCACCAAGTGC
CelII 126 GCTCAGC GC^T[ACGT]AGC
1615 GCTGAGC
Cfr10I 300 GCCGGT [GA]^CCGG[CT]
915 GCCGGT
HgaI 161 GCGTC GACGC(5/10)
252 GCGTC
MluNI 149 TGGCCA TGG^CCA
1007 TGGCCA
MscI 149 TGGCCA TGG^CCA
1007 TGGCCA
MseI 701 TTAA T^TAA
749 TTAA
NcoI 1694 CCATGG C^CATGG
2007 CCATGG
PleI 677 GACTC GAGTC(4/5)
1641 GACTC
RsaI 603 GTAC GT^AC
639 GTAC
SphI 909 GCATGC GCATG^C
1866 GCATGC
TaqI 565 TCGA T^CGA
2048 TCGA
TfiI 1622 GATTC G^A[AT]TC
2106 GAATC
Tru9I 701 TTAA T^TAA
749 TTAA
TthHB8I 565 TCGA T^CGA
2048 TCGA
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# 3 OR MORE CUT ENZYMES ( 83 enzymes) #
#########################################
AccI AciI AluI AlwNI AocI
ApaI AspHI AvaI AvaII BanI
BanII BbvI BcnI BmyI BsaJI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrI BssKI
BstF5I BstNI Bsu36I Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI Eco81I EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeIII MboI
MboII MnlI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
NspI PpuMI PstI Sau3AI Sau96I
ScrFI SfaNI SfcI SmaI SmlI
StuI StyI TseI Tsp45I Tsp509I
TspRI XmaCI XmaI