Restriction map for
pF1KSDA0615
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# NO CUT ENZYMES ( 111 enzymes) #
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AatII Acc65I AccI AccIII AcyI
AflII AgeI AhdI Alw44I AocI
Aor51HI ApaI ApaLI AscI Asp718I
AspEI AviII AvrII BalI BamHI
BbeI BbrPI BfrI BlnI BlpI
Bpu1102I BsaAI BsaBI BsaHI BsaWI
BseAI BsgI BsiWI BsmBI Bsp1407I
BspDI BspEI BspLU11I BsrBI BsrFI
BsrGI BssSI Bst1107I BstBI BstEII
BstPI BstZ17I Bsu36I CelII Cfr10I
ClaI CpoI DrdI Eam1105I Eco47III
Eco81I EcoNI EcoO65I EcoRV EcoT22I
FseI FspI HgaI Hin1I HincII
HindII HpaI KasI KpnI MamI
MluI MluNI MroI MscI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI PshAI
PvuI RsrII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmlI SnaBI SpeI SphI
Sse8387I SspBI StuI SwaI XcmI
XhoI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AclI 1631 AACGTT AA^CGTT
AflIII 2237 ACACGT A^C[GA][CT]GT
AseI 1378 ATTAAT AT^TAAT
AsnI 1378 ATTAAT AT^TAAT
AspI 754 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 8 CCCGGG C^[CT]CG[GA]G
BanI 2401 GGCACC G^G[CT][GA]CC
BbsI 1177 GAAGAC GAAGAC(2/6)
BcgI 24 CGAGTTCACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 1749 TGATCA T^GATCA
BpuAI 1177 GAAGAC GAAGAC(2/6)
BsaI 1213 GAGACC GGTCTC(1/5)
BsiEI 72 CGGCCG CG[GA][CT]^CG
BsoBI 8 CCCGGG C^[CT]CG[GA]G
BspHI 2106 TCATGA T^CATGA
BspMI 2525 ACCTGC ACCTGC(4/8)
BsrDI 1874 GCAATG GCAATG(2/0)
BstAPI 2520 GCAGAACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 2675 CCATGGTGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraI 1060 TTTAAA TTT^AAA
DraIII 569 CACAAGGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 72 CGGCCG [CT]^GGCC[GA]
EagI 72 CGGCCG C^GGCCG
EclXI 72 CGGCCG C^GGCCG
Eco52I 72 CGGCCG C^GGCCG
EcoRI 475 GAATTC G^AATTC
FbaI 1749 TGATCA T^GATCA
HaeII 190 AGCGCC [GA]GCGC^[CT]
KspI 69 CCGCGG CCGC^GG
MfeI 1928 CAATTG C^AATTG
MunI 1928 CAATTG C^AATTG
PflFI 754 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 1740 AGGACCT [GA]G^G[AT]CC[CT]
PshBI 1378 ATTAAT AT^TAAT
Psp1406I 1631 AACGTT AA^CGTT
RcaI 2106 TCATGA T^CATGA
SacI 2047 GAGCTC GAGCT^C
SacII 69 CCGCGG CCGC^GG
SmaI 8 CCCGGG CCC^GGG
Tth111I 754 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 676 TCTAGA T^CTAGA
XmaCI 8 CCCGGG C^CCGGG
XmaI 8 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 15 enzymes) #
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BanII 2047 GAGCTC G[GA]GC[CT]^C
2450 GAGCCC
BciVI 312 GGATAC GTATCC(6/5)
1206 GTATCC
BglI 86 GCCTGTTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
347 GCCTTCTTGGC
BsmI 651 GAATGC GAATGC(1/-1)
332 GCATTC
BssHII 116 GCGCGC G^CGCGC
145 GCGCGC
DraII 83 AGGGCCT [GA]G^G[ACGT]CC[CT]
1740 AGGACCT
EarI 700 GAAGAG CTCTTC(1/4)
817 GAAGAG
EcoO109I 83 AGGGCCT [GA]G^G[ACGT]CC[CT]
1740 AGGACCT
FauI 142 CCCGC CCCGC(4/6)
167 GCGGG
HindIII 300 AAGCTT A^AGCTT
1951 AAGCTT
Ksp632I 700 GAAGAG CTCTTC(1/4)
817 GAAGAG
MaeII 1632 ACGT A^CGT
2239 ACGT
NspI 257 ACATGC [GA]CATG^[CT]
2372 GCATGT
PflMI 793 CCAATAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2625 CCAGATCTTGG
Van91I 793 CCAATAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2625 CCAGATCTTGG
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# 3 OR MORE CUT ENZYMES ( 89 enzymes) #
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AccII AciI AcsI AfaI AluI
AlwI AlwNI ApoI Asp700I AspHI
AvaII BbvCI BbvI BcnI BfaI
BglII BmyI BpmI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BsrI BssKI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DsaI EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
MaeI MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NcoI
NdeII NlaIII NlaIV PleI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SspI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII XmnI