##################################
# NO CUT ENZYMES (   65 enzymes) #
##################################

Acc65I      AccIII      AclI        AcsI        AflII       
AhdI        ApoI        AscI        AseI        AsnI        
Asp718I     AspEI       AspI        AvrII       BamHI       
BclI        BfrI        BlnI        BsaI        BseAI       
BsiWI       BspEI       BspLU11I    BsrDI       Bst1107I    
BstBI       BstZ17I     CpoI        DraI        DrdI        
Eam1105I    EcoRI       EcoRV       EcoT22I     FbaI        
FseI        HpaI        KpnI        MfeI        MroI        
MunI        NdeI        NheI        NotI        NruI        
NsiI        NspV        PacI        PaeR7I      PflFI       
PmeI        PshBI       Psp1406I    PvuI        RsrII       
SbfI        SfuI        SnaBI       SpeI        Sse8387I    
SspI        SwaI        Tth111I     XbaI        XhoI        


###################################
# ONE CUT ENZYMES (   31 enzymes) #
###################################

AccI         4816  GTCGAC               GT^[AC][GT]AC
AgeI          640  ACCGGT               A^CCGGT
Aor51HI      5507  AGCGCT               AGC^GCT
AviII        3077  TGCGCA               TGC^GCA
BbrPI        2032  CACGTG               CAC^GTG
BbvCI        3457  GCTGAGG              CCTCAGC(-5/-2)
BciVI        5180  GTATCC               GTATCC(6/5)
BfaI          580  CTAG                 C^TAG
BsmI         5559  GAATGC               GAATGC(1/-1)
Bsp1407I     4505  TGTACA               T^GTACA
BspDI        1828  ATCGAT               AT^CGAT
BspHI        4385  TCATGA               T^CATGA
BsrGI        4505  TGTACA               T^GTACA
ClaI         1828  ATCGAT               AT^CGAT
Eco47III     5507  AGCGCT               AGC^GCT
EcoNI        5160  CCTGTTCAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FspI         3077  TGCGCA               TGC^GCA
HindIII      4903  AAGCTT               A^AGCTT
MaeI          580  CTAG                 C^TAG
MseI         4907  TTAA                 T^TAA
PinAI         640  ACCGGT               A^CCGGT
PmaCI        2032  CACGTG               CAC^GTG
PmlI         2032  CACGTG               CAC^GTG
RcaI         4385  TCATGA               T^CATGA
SacI         3031  GAGCTC               GAGCT^C
SalI         4816  GTCGAC               G^TCGAC
SexAI        2426  ACCAGGT              A^CC[AT]GGT
SfiI          270  GGCCGAGGCGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SgrAI         978  CGCCGGCG             C[GA]^CCGG[CT]G
SspBI        4505  TGTACA               T^GTACA
Tru9I        4907  TTAA                 T^TAA


###################################
# TWO CUT ENZYMES (   12 enzymes) #
###################################

AatII        1608  GACGTC               GACGT^C
             4813  GACGTC              
AocI         2245  CCTCAGG              CC^T[ACGT]AGG
             3109  CCTGAGG             
Asp700I      5013  GAAGTATTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             5595  GAATGAGTTC          
BcgI         3968  GCAAAGGCTTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
             5073  GCAGAGCCATCG        
BstAPI       1211  GCACCGCCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             3092  GCATCGCCTGC         
Bsu36I       2245  CCTCAGG              CC^T[ACGT]AGG
             3109  CCTGAGG             
Eco81I       2245  CCTCAGG              CC^T[ACGT]AGG
             3109  CCTGAGG             
MluI          563  ACGCGT               A^CGCGT
              887  ACGCGT              
PshAI        2590  GACCTGGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
             4740  GACACAGGTC          
ScaI         4370  AGTACT               AGT^ACT
             5213  AGTACT              
SphI          533  GCATGC               GCATG^C
             1505  GCATGC              
XmnI         5013  GAAGTATTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             5595  GAATGAGTTC          


#########################################
# 3 OR MORE CUT ENZYMES (  150 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AflIII      
AluI        Alw44I      AlwI        AlwNI       ApaI        
ApaLI       AspHI       AvaI        AvaII       BalI        
BanI        BanII       BbeI        BbsI        BbvI        
BcnI        BglI        BglII       BlpI        BmyI        
BpmI        Bpu1102I    BpuAI       BsaAI       BsaBI       
BsaHI       BsaJI       BsaWI       BseRI       BsgI        
BsiEI       BsiHKAI     BsiYI       BslI        BsmAI       
BsmBI       BsmFI       BsoBI       Bsp1286I    BspMI       
BsrBI       BsrFI       BsrI        BssHII      BssKI       
BssSI       BstEII      BstF5I      BstNI       BstPI       
BstUI       BstXI       BstYI       Cac8I       CelII       
CfoI        Cfr10I      Cfr13I      DdeI        DpnII       
DraII       DraIII      DsaI        EaeI        EagI        
EarI        EclXI       Eco52I      EcoO109I    EcoO65I     
EcoRII      EcoT14I     FauI        Fnu4HI      FokI        
HaeII       HaeIII      HapII       HgaI        HhaI        
Hin1I       HinP1I      HincII      HindII      HinfI       
HpaII       HphI        ItaI        KasI        Ksp632I     
KspI        MaeII       MaeIII      MamI        MboI        
MboII       MflI        MluNI       MnlI        MscI        
MslI        MspA1I      MspI        MvaI        MvnI        
MwoI        NaeI        NarI        NciI        NcoI        
NdeII       NgoMIV      NlaIII      NlaIV       NspI        
PflMI       PleI        PpuMI       PstI        PvuII       
RsaI        SacII       SapI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        SfoI        SmaI        
SmlI        StuI        StyI        TaqI        TfiI        
TseI        Tsp45I      Tsp509I     TspRI       TthHB8I     
Van91I      XcmI        XhoII       XmaCI       XmaI