Restriction map for
pF1KSDA0638
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# NO CUT ENZYMES ( 77 enzymes) #
##################################
AatII AclI AcsI AgeI Alw44I
ApaLI ApoI AscI AseI AsnI
AspI AvrII BamHI BcgI BciVI
BclI BglII BlnI BsaBI BsiWI
Bsp1407I BspDI BsrGI BssHII Bst1107I
BstBI BstEII BstPI BstZ17I ClaI
CpoI DraI DrdI EagI EclXI
Eco52I EcoO65I EcoRI EcoRV EcoT22I
FbaI HpaI KspI MamI MfeI
MluI MunI NdeI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmeI PshAI PshBI Psp1406I
PvuI RsrII SacII SalI SbfI
ScaI SfuI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI Tth111I
XbaI XhoI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AccI 2761 GTAGAC GT^[AC][GT]AC
AcyI 718 GGCGCC G[GA]^CG[CT]C
AflII 1377 CTTAAG C^TTAAG
AflIII 3407 ACATGT A^C[GA][CT]GT
AhdI 3403 GACAACATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 1891 CCTGAGG CC^T[ACGT]AGG
Asp700I 1280 GAACATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 3403 GACAACATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 3944 TGCGCA TGC^GCA
BbeI 718 GGCGCC GGCGC^C
BfrI 1377 CTTAAG C^TTAAG
BsaHI 718 GGCGCC G[GA]^CG[CT]C
BsiEI 3840 CGACCG CG[GA][CT]^CG
BsmBI 3883 GAGACG CGTCTC(1/5)
BspHI 4124 TCATGA T^CATGA
BspLU11I 3407 ACATGT A^CATGT
BsrDI 299 GCAATG GCAATG(2/0)
BssSI 1961 CACGAG CACGAG(-5/-1)
BstXI 722 CCATGTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1891 CCTGAGG CC^T[ACGT]AGG
Eam1105I 3403 GACAACATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1891 CCTGAGG CC^T[ACGT]AGG
EcoNI 3537 CCTGGAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FseI 2475 GGCCGGCC GGCCGG^CC
FspI 3944 TGCGCA TGC^GCA
Hin1I 718 GGCGCC G[GA]^CG[CT]C
HincII 1118 GTCAAC GT[CT]^[GA]AC
HindII 1118 GTCAAC GT[CT]^[GA]AC
HindIII 2858 AAGCTT A^AGCTT
KasI 718 GGCGCC G^GCGCC
NarI 718 GGCGCC GG^CGCC
NheI 2139 GCTAGC G^CTAGC
RcaI 4124 TCATGA T^CATGA
SexAI 122 ACCTGGT A^CC[AT]GGT
SfiI 1339 GGCCGGCGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 718 GGCGCC GGC^GCC
SgrAI 1777 CACCGGCG C[GA]^CCGG[CT]G
SmaI 2609 CCCGGG CCC^GGG
Tsp509I 3644 AATT ^AATT
XmaCI 2609 CCCGGG C^CCGGG
XmaI 2609 CCCGGG C^CCGGG
XmnI 1280 GAACATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 26 enzymes) #
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Acc65I 852 GGTACC G^GTACC
2777 GGTACC
AccIII 2492 TCCGGA T^CCGGA
3683 TCCGGA
Asp718I 852 GGTACC G^GTACC
2777 GGTACC
BalI 1076 TGGCCA TGG^CCA
2543 TGGCCA
BbrPI 2176 CACGTG CAC^GTG
3751 CACGTG
BbvCI 3181 GCTGAGG CCTCAGC(-5/-2)
4105 GCTGAGG
BsaAI 2176 CACGTG [CT]AC^GT[GA]
3751 CACGTG
BsaWI 2492 TCCGGA [AT]^CCGG[AT]
3683 TCCGGA
BseAI 2492 TCCGGA T^CCGGA
3683 TCCGGA
BsmI 2619 GAATGC GAATGC(1/-1)
1130 GCATTC
BspEI 2492 TCCGGA T^CCGGA
3683 TCCGGA
BstYI 1434 AGATCC [GA]^GATC[CT]
2570 AGATCC
HgaI 4 GACGC GACGC(5/10)
1521 GACGC
KpnI 852 GGTACC GGTAC^C
2777 GGTACC
MaeII 2177 ACGT A^CGT
3752 ACGT
MflI 1434 AGATCC [GA]^GATC[CT]
2570 AGATCC
MluNI 1076 TGGCCA TGG^CCA
2543 TGGCCA
MroI 2492 TCCGGA T^CCGGA
3683 TCCGGA
MscI 1076 TGGCCA TGG^CCA
2543 TGGCCA
NaeI 1340 GCCGGC GCC^GGC
2476 GCCGGC
NgoMIV 1340 GCCGGC G^CCGGC
2476 GCCGGC
NspI 359 GCATGT [GA]CATG^[CT]
3407 ACATGT
PmaCI 2176 CACGTG CAC^GTG
3751 CACGTG
PmlI 2176 CACGTG CAC^GTG
3751 CACGTG
TfiI 432 GATTC G^A[AT]TC
484 GAATC
XhoII 1434 AGATCC [GA]^GATC[CT]
2570 AGATCC
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# 3 OR MORE CUT ENZYMES ( 113 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI Aor51HI ApaI AspHI AvaI
AvaII BanI BanII BbsI BbvI
BcnI BfaI BglI BlpI BmyI
BpmI Bpu1102I BpuAI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrBI BsrFI BsrI BssKI BstAPI
BstF5I BstNI BstUI Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DraIII DsaI EaeI EarI
Eco47III EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeIII MboI
MboII MnlI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NcoI NdeII NlaIII NlaIV PflMI
PleI PpuMI PstI PvuII RsaI
SacI SapI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StuI StyI
TaqI Tru9I TseI Tsp45I TspRI
TthHB8I Van91I XcmI