Restriction map for
pF1KSDA0639
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# NO CUT ENZYMES ( 53 enzymes) #
##################################
AatII Acc65I AgeI Alw44I ApaLI
AscI Asp718I AviII AvrII BlnI
BsaBI BsiWI Bsp1407I BspDI BspHI
BspLU11I BsrGI BssHII BstAPI BstBI
ClaI CpoI DrdI FseI FspI
KpnI MamI MluI NdeI NheI
NotI NruI NspV PacI PinAI
PmeI PshAI PvuI RcaI RsrII
SapI SbfI SexAI SfiI SfuI
SgrAI SnaBI SpeI Sse8387I SspBI
StuI SwaI XbaI
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# ONE CUT ENZYMES ( 26 enzymes) #
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AclI 2031 AACGTT AA^CGTT
AflII 2001 CTTAAG C^TTAAG
AflIII 2923 ACACGT A^C[GA][CT]GT
Aor51HI 1987 AGCGCT AGC^GCT
AseI 363 ATTAAT AT^TAAT
AsnI 363 ATTAAT AT^TAAT
Asp700I 466 GAAAAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BamHI 1674 GGATCC G^GATCC
BfrI 2001 CTTAAG C^TTAAG
BssSI 4879 CTCGTG CACGAG(-5/-1)
Bst1107I 3149 GTATAC GTA^TAC
BstZ17I 3149 GTATAC GTA^TAC
Eco47III 1987 AGCGCT AGC^GCT
EcoRV 1438 GATATC GAT^ATC
HindIII 3334 AAGCTT A^AGCTT
KspI 3686 CCGCGG CCGC^GG
NaeI 5031 GCCGGC GCC^GGC
NgoMIV 5031 GCCGGC G^CCGGC
PshBI 363 ATTAAT AT^TAAT
Psp1406I 2031 AACGTT AA^CGTT
SacI 5200 GAGCTC GAGCT^C
SacII 3686 CCGCGG CCGC^GG
SalI 547 GTCGAC G^TCGAC
SphI 4982 GCATGC GCATG^C
SspI 3919 AATATT AAT^ATT
XmnI 466 GAAAAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 28 enzymes) #
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AspHI 1900 GAGCAC G[AT]GC[AT]^C
5200 GAGCTC
AspI 4728 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
5083 GACGAGGTC
BalI 2405 TGGCCA TGG^CCA
5423 TGGCCA
BbrPI 2500 CACGTG CAC^GTG
5184 CACGTG
BciVI 32 GGATAC GTATCC(6/5)
4240 GTATCC
BclI 174 TGATCA T^GATCA
2772 TGATCA
BglII 4179 AGATCT A^GATCT
4580 AGATCT
BsiHKAI 1900 GAGCAC G[AT]GC[AT]^C
5200 GAGCTC
BsmBI 4439 CGTCTC CGTCTC(1/5)
4716 CGTCTC
BsrBI 3187 CCGCTC CCGCTC(-3/-3)
3733 GAGCGG
DraI 430 TTTAAA TTT^AAA
1398 TTTAAA
DraIII 1066 CACGCTGTG CAC[ACGT][ACGT][ACGT]^GTG
2656 CACTTTGTG
EcoRI 1460 GAATTC G^AATTC
2968 GAATTC
EcoT22I 287 ATGCAT ATGCA^T
3290 ATGCAT
FbaI 174 TGATCA T^GATCA
2772 TGATCA
MfeI 2843 CAATTG C^AATTG
3325 CAATTG
MluNI 2405 TGGCCA TGG^CCA
5423 TGGCCA
MscI 2405 TGGCCA TGG^CCA
5423 TGGCCA
MunI 2843 CAATTG C^AATTG
3325 CAATTG
NcoI 974 CCATGG C^CATGG
1581 CCATGG
NsiI 287 ATGCAT ATGCA^T
3290 ATGCAT
PaeR7I 2419 CTCGAG C^TCGAG
4909 CTCGAG
PflFI 4728 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
5083 GACGAGGTC
PmaCI 2500 CACGTG CAC^GTG
5184 CACGTG
PmlI 2500 CACGTG CAC^GTG
5184 CACGTG
ScaI 3311 AGTACT AGT^ACT
5249 AGTACT
Tth111I 4728 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
5083 GACGAGGTC
XhoI 2419 CTCGAG C^TCGAG
4909 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 151 enzymes) #
#########################################
AccI AccII AccIII AciI AcsI
AcyI AfaI AhdI AluI AlwI
AlwNI AocI ApaI ApoI AspEI
AvaI AvaII BanI BanII BbeI
BbsI BbvCI BbvI BcgI BcnI
BfaI BglI BlpI BmyI BpmI
Bpu1102I BpuAI BsaAI BsaHI BsaI
BsaJI BsaWI BseAI BseRI BsgI
BsiEI BsiYI BslI BsmAI BsmFI
BsmI BsoBI Bsp1286I BspEI BspMI
BsrDI BsrFI BsrI BssKI BstEII
BstF5I BstNI BstPI BstUI BstXI
BstYI Bsu36I Cac8I CelII CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EagI Eam1105I EarI
EclXI Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HinfI HpaI HpaII HphI ItaI
KasI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MroI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NarI NciI NdeII
NlaIII NlaIV NspI PflMI PleI
PpuMI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SfoI
SmaI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XhoII XmaCI
XmaI