Restriction map for
pF1KSDA0644
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# NO CUT ENZYMES ( 86 enzymes) #
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AatII Acc65I AccIII AclI AflII
AgeI AhdI AscI AseI AsnI
Asp718I AspEI AvrII BbeI BbrPI
BcgI BciVI BclI BfrI BglII
BlnI BsaAI BsaBI BseAI BsiWI
BsmI Bsp1407I BspDI BspEI BspHI
BsrGI Bst1107I BstAPI BstBI BstZ17I
ClaI CpoI DraI DrdI Eam1105I
EcoRV EcoT22I FbaI FseI HindIII
HpaI KasI KpnI MamI MfeI
MluI MroI MseI MunI NarI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI RcaI RsrII ScaI
SfoI SfuI SmlI SnaBI SpeI
SphI SspBI SspI SwaI Tru9I
XhoI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AcsI 2341 GAATTC [GA]^AATT[CT]
AflIII 2219 ACATGT A^C[GA][CT]GT
Alw44I 120 GTGCAC G^TGCAC
AocI 808 CCTGAGG CC^T[ACGT]AGG
ApaLI 120 GTGCAC G^TGCAC
ApoI 2341 GAATTC [GA]^AATT[CT]
Asp700I 588 GAACGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 514 GACGCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 1881 TGGCCA TGG^CCA
BanI 2254 GGCACC G^G[CT][GA]CC
BbvCI 1256 CCTCAGC CCTCAGC(-5/-2)
BsaI 1333 GGTCTC GGTCTC(1/5)
BspLU11I 2219 ACATGT A^CATGT
BsrDI 929 GCAATG GCAATG(2/0)
BssSI 31 CTCGTG CACGAG(-5/-1)
BstEII 2043 GGTCACC G^GT[ACGT]ACC
BstPI 2043 GGTCACC G^GT[ACGT]ACC
BstXI 1415 CCAGGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 808 CCTGAGG CC^T[ACGT]AGG
EarI 328 GAAGAG CTCTTC(1/4)
Eco81I 808 CCTGAGG CC^T[ACGT]AGG
EcoO65I 2043 GGTCACC G^GT[ACGT]ACC
EcoRI 2341 GAATTC G^AATTC
Ksp632I 328 GAAGAG CTCTTC(1/4)
MaeIII 2044 GTCAC ^GT[ACGT]AC
MluNI 1881 TGGCCA TGG^CCA
MscI 1881 TGGCCA TGG^CCA
NcoI 2249 CCATGG C^CATGG
NspI 2219 ACATGT [GA]CATG^[CT]
PflFI 514 GACGCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 237 CCACCGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SacI 985 GAGCTC GAGCT^C
SalI 2077 GTCGAC G^TCGAC
SapI 328 GAAGAGC GCTCTTC(1/4)
SbfI 1327 CCTGCAGG CCTGCA^GG
SexAI 1970 ACCTGGT A^CC[AT]GGT
Sse8387I 1327 CCTGCAGG CCTGCA^GG
TfiI 2282 GATTC G^A[AT]TC
Tsp45I 2044 GTCAC ^GT[GC]AC
Tsp509I 2342 AATT ^AATT
Tth111I 514 GACGCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 237 CCACCGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 1065 TCTAGA T^CTAGA
XcmI 1692 CCAGTCCGACGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 588 GAACGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 19 enzymes) #
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AccI 1909 GTCTAC GT^[AC][GT]AC
2077 GTCGAC
BbsI 520 GTCTTC GAAGAC(2/6)
1156 GTCTTC
BpuAI 520 GTCTTC GAAGAC(2/6)
1156 GTCTTC
BsaWI 566 ACCGGA [AT]^CCGG[AT]
2052 ACCGGA
BsrBI 285 CCGCTC CCGCTC(-3/-3)
924 GAGCGG
DraIII 175 CACGACGTG CAC[ACGT][ACGT][ACGT]^GTG
2307 CACCGTGTG
EagI 1301 CGGCCG C^GGCCG
1837 CGGCCG
EclXI 1301 CGGCCG C^GGCCG
1837 CGGCCG
Eco52I 1301 CGGCCG C^GGCCG
1837 CGGCCG
EcoNI 351 CCTCTTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2049 CCTACCGGAGG
HincII 2077 GTCGAC GT[CT]^[GA]AC
2113 GTCAAC
HindII 2077 GTCGAC GT[CT]^[GA]AC
2113 GTCAAC
HphI 185 TCACC GGTGA(8/7)
2045 TCACC
KspI 441 CCGCGG CCGC^GG
1502 CCGCGG
MslI 2256 CACCGGCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2304 CACCACCGTG
SacII 441 CCGCGG CCGC^GG
1502 CCGCGG
SfiI 964 GGCCACCTGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
2136 GGCCTTGGCGGCC
SgrAI 1626 CGCCGGCG C[GA]^CCGG[CT]G
2256 CACCGGCG
StuI 812 AGGCCT AGG^CCT
1104 AGGCCT
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# 3 OR MORE CUT ENZYMES ( 108 enzymes) #
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AccII AciI AcyI AfaI AluI
AlwI AlwNI Aor51HI ApaI AspHI
AvaI AvaII AviII BamHI BanII
BbvI BcnI BfaI BglI BlpI
BmyI BpmI Bpu1102I BsaHI BsaJI
BseRI BsgI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI BsoBI
Bsp1286I BspMI BsrFI BsrI BssHII
BssKI BstF5I BstNI BstUI BstYI
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
Eco47III EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI FspI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII ItaI MaeI MaeII
MboI MboII MflI MnlI MspA1I
MspI MvaI MvnI MwoI NaeI
NciI NdeII NgoMIV NlaIII NlaIV
PleI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmaI
StyI TaqI TseI TspRI TthHB8I
XhoII XmaCI XmaI