Restriction map for
pF1KSDA0652
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# NO CUT ENZYMES ( 143 enzymes) #
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AatII AccIII AclI AcyI AflIII
AgeI AhdI Alw44I AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp700I AspEI AspI AvaI AviII
AvrII BalI BamHI BanII BbeI
BbrPI BcnI BglI BglII BlnI
BsaAI BsaHI BsaWI BseAI BsiEI
BsiWI BsmBI BsoBI BspDI BspEI
BspHI BspLU11I BsrBI BsrFI BssHII
BssSI Bst1107I BstAPI BstBI BstYI
BstZ17I Bsu36I CfoI Cfr10I ClaI
CpoI DrdI EaeI EagI Eam1105I
EarI EclXI Eco47III Eco52I Eco81I
EcoNI EcoRI EcoRV EcoT22I FseI
FspI HaeII HapII HgaI HhaI
Hin1I HinP1I HindIII HpaI HpaII
KasI Ksp632I KspI MaeII MfeI
MflI MluI MluNI MroI MscI
MspI MunI NaeI NarI NciI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspI StuI SwaI TaqI
Tth111I TthHB8I XbaI XhoI XhoII
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AccI 588 GTCTAC GT^[AC][GT]AC
AccII 1519 CGCG CG^CG
AflII 256 CTTAAG C^TTAAG
AlwNI 1250 CAGAGACTG CAG[ACGT][ACGT][ACGT]^CTG
AspHI 1451 GAGCAC G[AT]GC[AT]^C
BbvCI 1420 CCTCAGC CCTCAGC(-5/-2)
BcgI 1521 CGAGTTTGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 636 TGATCA T^GATCA
BfaI 395 CTAG C^TAG
BfrI 256 CTTAAG C^TTAAG
BlpI 1425 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1425 GCTGAGC GC^T[ACGT]AGC
BsaBI 1216 GATACCGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 1099 GAGACC GGTCTC(1/5)
BsiHKAI 1451 GAGCAC G[AT]GC[AT]^C
BsmI 580 GCATTC GAATGC(1/-1)
Bsp1407I 350 TGTACA T^GTACA
BspMI 988 ACCTGC ACCTGC(4/8)
BsrDI 365 CATTGC GCAATG(2/0)
BsrGI 350 TGTACA T^GTACA
BstEII 1152 GGTGACC G^GT[ACGT]ACC
BstPI 1152 GGTGACC G^GT[ACGT]ACC
BstUI 1519 CGCG CG^CG
CelII 1425 GCTGAGC GC^T[ACGT]AGC
DraI 1345 TTTAAA TTT^AAA
DraIII 640 CACTTTGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoO65I 1152 GGTGACC G^GT[ACGT]ACC
FauI 1298 GCGGG CCCGC(4/6)
FbaI 636 TGATCA T^GATCA
HaeIII 1077 GGCC GG^CC
HincII 1132 GTCAAC GT[CT]^[GA]AC
HindII 1132 GTCAAC GT[CT]^[GA]AC
MaeI 395 CTAG C^TAG
MamI 1216 GATACCGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MvnI 1519 CGCG CG^CG
PflMI 897 CCAGCTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SexAI 1148 ACCAGGT A^CC[AT]GGT
SfaNI 1528 GATGC GCATC(5/9)
SspBI 350 TGTACA T^GTACA
Van91I 897 CCAGCTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
Acc65I 927 GGTACC G^GTACC
958 GGTACC
AciI 1518 CCGC CCGC(-3/-1)
1298 GCGG
AcsI 52 AAATTT [GA]^AATT[CT]
1200 GAATTT
AlwI 1222 GATCC GGATC(4/5)
835 GGATC
ApoI 52 AAATTT [GA]^AATT[CT]
1200 GAATTT
Asp718I 927 GGTACC G^GTACC
958 GGTACC
BbsI 736 GAAGAC GAAGAC(2/6)
1468 GAAGAC
BciVI 1041 GGATAC GTATCC(6/5)
1215 GGATAC
BpuAI 736 GAAGAC GAAGAC(2/6)
1468 GAAGAC
BseRI 666 CTCCTC GAGGAG(10/8)
1262 CTCCTC
BsgI 990 CTGCAC GTGCAG(16/14)
1279 CTGCAC
BstF5I 171 CATCC GGATG(2/0)
782 CATCC
FokI 171 CATCC GGATG(9/13)
782 CATCC
KpnI 927 GGTACC GGTAC^C
958 GGTACC
PleI 739 GACTC GAGTC(4/5)
1477 GACTC
PstI 221 CTGCAG CTGCA^G
1546 CTGCAG
Tsp45I 400 GTGAC ^GT[GC]AC
1153 GTGAC
XcmI 87 CCAGGCTCGGCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
945 CCAGCCTGTCCATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 57 enzymes) #
#########################################
AfaI AluI AvaII BanI BbvI
BmyI BpmI BsaJI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrI BssKI
BstNI BstXI Cac8I Cfr13I DdeI
DpnII DraII DsaI EcoO109I EcoRII
EcoT14I Fnu4HI HinfI HphI ItaI
MaeIII MboI MboII MnlI MseI
MslI MspA1I MvaI MwoI NcoI
NdeII NlaIII NlaIV PpuMI PvuII
RsaI Sau3AI Sau96I ScrFI SfcI
SmlI StyI TfiI Tru9I TseI
Tsp509I TspRI