Restriction map for
pF1KSDA0654
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# NO CUT ENZYMES ( 94 enzymes) #
##################################
AatII Acc65I AclI AflII AgeI
AhdI Alw44I ApaLI AscI AseI
AsnI Asp718I AspEI AspI AvrII
BbeI BbrPI BbsI BcgI BciVI
BclI BfrI BglII BlnI BpuAI
BsaAI BsiEI BsiWI Bsp1407I BspDI
BspHI BspLU11I BspMI BsrDI BsrGI
Bst1107I BstBI BstEII BstPI BstZ17I
ClaI DraI DrdI EagI Eam1105I
EclXI Eco52I EcoO65I EcoRV EcoT22I
FbaI HincII HindII HpaI KasI
KpnI KspI MfeI MluI MunI
NarI NcoI NotI NruI NsiI
NspV PacI PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI SacII SalI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SnaBI SpeI Sse8387I SspBI
SspI SwaI Tth111I XbaI
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# ONE CUT ENZYMES ( 27 enzymes) #
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AccI 3017 GTCTAC GT^[AC][GT]AC
AccIII 3566 TCCGGA T^CCGGA
AfaI 3199 GTAC GT^AC
AflIII 2756 ACGTGT A^C[GA][CT]GT
AviII 392 TGCGCA TGC^GCA
BamHI 1590 GGATCC G^GATCC
BbvCI 2720 CCTCAGC CCTCAGC(-5/-2)
BseAI 3566 TCCGGA T^CCGGA
BsmI 3276 GAATGC GAATGC(1/-1)
BspEI 3566 TCCGGA T^CCGGA
BssHII 735 GCGCGC G^CGCGC
CpoI 3563 CGGTCCG CG^G[AT]CCG
DraIII 3007 CACAGGGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoRI 3307 GAATTC G^AATTC
FseI 2120 GGCCGGCC GGCCGG^CC
FspI 392 TGCGCA TGC^GCA
HindIII 1411 AAGCTT A^AGCTT
MroI 3566 TCCGGA T^CCGGA
MseI 2960 TTAA T^TAA
NdeI 2843 CATATG CA^TATG
NspI 2555 GCATGC [GA]CATG^[CT]
PaeR7I 550 CTCGAG C^TCGAG
RsaI 3199 GTAC GT^AC
RsrII 3563 CGGTCCG CG^G[AT]CCG
SphI 2555 GCATGC GCATG^C
Tru9I 2960 TTAA T^TAA
XhoI 550 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 26 enzymes) #
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AcyI 970 GACGCC G[GA]^CG[CT]C
1066 GACGCC
AocI 2086 CCTAAGG CC^T[ACGT]AGG
3326 CCTTAGG
Aor51HI 929 AGCGCT AGC^GCT
1328 AGCGCT
Asp700I 3303 GAAGGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3393 GAAGATGTTC
BlpI 219 GCTCAGC GC^T[ACGT]AGC
1311 GCTGAGC
Bpu1102I 219 GCTCAGC GC^T[ACGT]AGC
1311 GCTGAGC
BsaBI 1473 GATGGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2646 GATCGGCATC
BsaHI 970 GACGCC G[GA]^CG[CT]C
1066 GACGCC
BsaWI 3059 ACCGGA [AT]^CCGG[AT]
3566 TCCGGA
BstXI 665 CCAATGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3528 CCAGACTTCTGG
Bsu36I 2086 CCTAAGG CC^T[ACGT]AGG
3326 CCTTAGG
CelII 219 GCTCAGC GC^T[ACGT]AGC
1311 GCTGAGC
Eco47III 929 AGCGCT AGC^GCT
1328 AGCGCT
Eco81I 2086 CCTAAGG CC^T[ACGT]AGG
3326 CCTTAGG
Hin1I 970 GACGCC G[GA]^CG[CT]C
1066 GACGCC
MamI 1473 GATGGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2646 GATCGGCATC
MslI 396 CATGACCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2523 CACCGTGGTG
NaeI 38 GCCGGC GCC^GGC
2121 GCCGGC
NgoMIV 38 GCCGGC G^CCGGC
2121 GCCGGC
NheI 1000 GCTAGC G^CTAGC
2412 GCTAGC
SmaI 426 CCCGGG CCC^GGG
3545 CCCGGG
StuI 2186 AGGCCT AGG^CCT
2480 AGGCCT
Tsp45I 97 GTCAC ^GT[GC]AC
387 GTCAC
XmaCI 426 CCCGGG C^CCGGG
3545 CCCGGG
XmaI 426 CCCGGG C^CCGGG
3545 CCCGGG
XmnI 3303 GAAGGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3393 GAAGATGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 111 enzymes) #
#########################################
AccII AciI AcsI AluI AlwI
AlwNI ApaI ApoI AspHI AvaI
AvaII BalI BanI BanII BbvI
BcnI BfaI BglI BmyI BpmI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmBI BsmFI
BsoBI Bsp1286I BsrBI BsrFI BsrI
BssKI BssSI BstAPI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV PflMI PleI
PpuMI PstI PvuII SacI SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TaqI TfiI TseI
Tsp509I TspRI TthHB8I Van91I XcmI
XhoII