Restriction map for
pF1KSDA0658
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# NO CUT ENZYMES ( 96 enzymes) #
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AatII Acc65I AccIII AclI AfaI
AflII AgeI AhdI AscI AseI
AsnI Asp718I AspEI AviII AvrII
BbrPI BcgI BciVI BclI BfaI
BfrI BglII BlnI BlpI Bpu1102I
BsaAI BsaBI BsaWI BseAI BsiWI
BsmBI Bsp1407I BspDI BspEI BspLU11I
BsrDI BsrGI BssSI Bst1107I BstAPI
BstBI BstZ17I CelII ClaI CpoI
DraI Eam1105I EcoNI EcoRV EcoT22I
FbaI FseI FspI HgaI HindIII
HpaI KpnI MaeI MamI MfeI
MluI MroI MunI NcoI NdeI
NheI NotI NruI NsiI NspV
PacI PinAI PmaCI PmeI PmlI
PshBI Psp1406I PvuI RsaI RsrII
SacI SalI SapI SbfI ScaI
SfiI SfuI SgrAI SnaBI SpeI
Sse8387I SspBI SspI SwaI TfiI
XbaI
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# ONE CUT ENZYMES ( 62 enzymes) #
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AccI 1550 GTCTAC GT^[AC][GT]AC
AcsI 438 GAATTC [GA]^AATT[CT]
AcyI 39 GGCGCC G[GA]^CG[CT]C
AflIII 302 ACGTGT A^C[GA][CT]GT
Alw44I 73 GTGCAC G^TGCAC
AlwNI 226 CAGTCTCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1027 CCTGAGG CC^T[ACGT]AGG
Aor51HI 509 AGCGCT AGC^GCT
ApaI 1730 GGGCCC GGGCC^C
ApaLI 73 GTGCAC G^TGCAC
ApoI 438 GAATTC [GA]^AATT[CT]
Asp700I 1365 GAAAGCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 73 GTGCAC G[AT]GC[AT]^C
BamHI 1112 GGATCC G^GATCC
BanI 39 GGCGCC G^G[CT][GA]CC
BbeI 39 GGCGCC GGCGC^C
BbsI 1579 GAAGAC GAAGAC(2/6)
BpmI 640 CTGGAG CTGGAG(16/14)
BpuAI 1579 GAAGAC GAAGAC(2/6)
BsaHI 39 GGCGCC G[GA]^CG[CT]C
BsgI 1637 GTGCAG GTGCAG(16/14)
BsiEI 125 CGGCCG CG[GA][CT]^CG
BsiHKAI 73 GTGCAC G[AT]GC[AT]^C
BsmI 1401 GAATGC GAATGC(1/-1)
BsrBI 1182 GAGCGG CCGCTC(-3/-3)
BsrFI 1487 GCCGGC [GA]^CCGG[CT]
BssHII 139 GCGCGC G^CGCGC
BstEII 564 GGTGACC G^GT[ACGT]ACC
BstPI 564 GGTGACC G^GT[ACGT]ACC
BstXI 676 CCAGCTGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1027 CCTGAGG CC^T[ACGT]AGG
Cfr10I 1487 GCCGGC [GA]^CCGG[CT]
DrdI 1162 GACCTCTGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 125 CGGCCG C^GGCCG
EclXI 125 CGGCCG C^GGCCG
Eco47III 509 AGCGCT AGC^GCT
Eco52I 125 CGGCCG C^GGCCG
Eco81I 1027 CCTGAGG CC^T[ACGT]AGG
EcoO65I 564 GGTGACC G^GT[ACGT]ACC
EcoRI 438 GAATTC G^AATTC
Hin1I 39 GGCGCC G[GA]^CG[CT]C
HincII 1468 GTCAAC GT[CT]^[GA]AC
HindII 1468 GTCAAC GT[CT]^[GA]AC
KasI 39 GGCGCC G^GCGCC
KspI 1155 CCGCGG CCGC^GG
MaeII 302 ACGT A^CGT
NaeI 1487 GCCGGC GCC^GGC
NarI 39 GGCGCC GG^CGCC
NgoMIV 1487 GCCGGC G^CCGGC
NspI 1131 GCATGC [GA]CATG^[CT]
PaeR7I 1611 CTCGAG C^TCGAG
PshAI 1582 GACCTCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacII 1155 CCGCGG CCGC^GG
SexAI 1707 ACCTGGT A^CC[AT]GGT
SfoI 39 GGCGCC GGC^GCC
SmaI 1733 CCCGGG CCC^GGG
SmlI 1611 CTCGAG C^T[CT][GA]AG
SphI 1131 GCATGC GCATG^C
XhoI 1611 CTCGAG C^TCGAG
XmaCI 1733 CCCGGG C^CCGGG
XmaI 1733 CCCGGG C^CCGGG
XmnI 1365 GAAAGCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AspI 668 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1544 GACTCTGTC
AvaI 1611 CTCGAG C^[CT]CG[GA]G
1733 CCCGGG
BbvCI 807 CCTCAGC CCTCAGC(-5/-2)
1048 GCTGAGG
BsoBI 1611 CTCGAG C^[CT]CG[GA]G
1733 CCCGGG
BspHI 395 TCATGA T^CATGA
1085 TCATGA
BspMI 818 ACCTGC ACCTGC(4/8)
1352 ACCTGC
DraIII 1591 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG
1706 CACCTGGTG
EarI 66 CTCTTC CTCTTC(1/4)
847 CTCTTC
Ksp632I 66 CTCTTC CTCTTC(1/4)
847 CTCTTC
MseI 253 TTAA T^TAA
1493 TTAA
MslI 396 CATGAAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1440 CATTGGTGTG
PflFI 668 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1544 GACTCTGTC
PflMI 573 CCAGCAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1116 CCACCACCTGG
PpuMI 866 GGGACCT [GA]G^G[AT]CC[CT]
1160 GGGACCT
PstI 588 CTGCAG CTGCA^G
1260 CTGCAG
RcaI 395 TCATGA T^CATGA
1085 TCATGA
SfcI 588 CTGCAG C^T[GA][CT]AG
1260 CTGCAG
StuI 740 AGGCCT AGG^CCT
804 AGGCCT
Tru9I 253 TTAA T^TAA
1493 TTAA
Tth111I 668 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1544 GACTCTGTC
Van91I 573 CCAGCAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1116 CCACCACCTGG
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# 3 OR MORE CUT ENZYMES ( 79 enzymes) #
#########################################
AccII AciI AluI AlwI AvaII
BalI BanII BbvI BcnI BglI
BmyI BsaI BsaJI BseRI BsiYI
BslI BsmAI BsmFI Bsp1286I BsrI
BssKI BstF5I BstNI BstUI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI MaeIII MboI
MboII MflI MluNI MnlI MscI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV PleI
PvuII Sau3AI Sau96I ScrFI SfaNI
StyI TaqI TseI Tsp45I Tsp509I
TspRI TthHB8I XcmI XhoII