Restriction map for
pF1KSDA0663
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# NO CUT ENZYMES ( 118 enzymes) #
##################################
AatII Acc65I AccI AccII AclI
AcyI AgeI AhdI Aor51HI ApaI
AscI Asp718I AspEI AspI AvaI
AviII AvrII BalI BbeI BbrPI
BglII BlnI BlpI Bpu1102I BsaAI
BsaHI BsiEI BsiWI BsmI BsoBI
Bsp1407I BspDI BspHI BsrBI BsrFI
BsrGI BssHII Bst1107I BstBI BstEII
BstPI BstUI BstXI BstZ17I CelII
Cfr10I ClaI CpoI DraI DraIII
DsaI EaeI EagI Eam1105I EclXI
Eco47III Eco52I EcoO65I EcoRI EcoRV
EcoT22I FseI FspI Hin1I HindIII
HpaI KasI KpnI KspI MfeI
MluI MluNI MscI MunI MvnI
NaeI NarI NcoI NdeI NgoMIV
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI Psp1406I PvuI RcaI RsrII
SacII SalI SapI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI SspBI SspI
StuI SwaI Tth111I XbaI XcmI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 53 enzymes) #
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AccIII 590 TCCGGA T^CCGGA
AfaI 47 GTAC GT^AC
AflII 991 CTTAAG C^TTAAG
AflIII 43 ACATGT A^C[GA][CT]GT
Alw44I 1405 GTGCAC G^TGCAC
AocI 1871 CCTCAGG CC^T[ACGT]AGG
ApaLI 1405 GTGCAC G^TGCAC
AseI 469 ATTAAT AT^TAAT
AsnI 469 ATTAAT AT^TAAT
BamHI 2373 GGATCC G^GATCC
BanI 1817 GGCACC G^G[CT][GA]CC
BbvCI 397 CCTCAGC CCTCAGC(-5/-2)
BcgI 139 CGACAGGTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1281 GGATAC GTATCC(6/5)
BclI 501 TGATCA T^GATCA
BcnI 2217 CCCGG CC^[GC]GG
BfrI 991 CTTAAG C^TTAAG
BglI 2089 GCCAAAAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaBI 496 GATGATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 590 TCCGGA [AT]^CCGG[AT]
BseAI 590 TCCGGA T^CCGGA
BsmBI 1487 CGTCTC CGTCTC(1/5)
BsmFI 2113 GTCCC GGGAC(10/14)
BspEI 590 TCCGGA T^CCGGA
BspLU11I 43 ACATGT A^CATGT
BsrDI 2037 CATTGC GCAATG(2/0)
BssSI 1495 CACGAG CACGAG(-5/-1)
BstAPI 1398 GCAGGAGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 1871 CCTCAGG CC^T[ACGT]AGG
DrdI 2131 GACAAATCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1871 CCTCAGG CC^T[ACGT]AGG
EcoNI 4 CCTAATCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FauI 2115 CCCGC CCCGC(4/6)
FbaI 501 TGATCA T^GATCA
HaeII 128 GGCGCT [GA]GCGC^[CT]
HgaI 1416 GACGC GACGC(5/10)
HincII 1645 GTTGAC GT[CT]^[GA]AC
HindII 1645 GTTGAC GT[CT]^[GA]AC
MamI 496 GATGATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 590 TCCGGA T^CCGGA
MslI 1794 CACTGCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NciI 2217 CCCGG CC^[GC]GG
NheI 343 GCTAGC G^CTAGC
PflMI 1851 CCAGAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 582 GACTTCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 469 ATTAAT AT^TAAT
RsaI 47 GTAC GT^AC
SacI 1461 GAGCTC GAGCT^C
SbfI 1375 CCTGCAGG CCTGCA^GG
Sse8387I 1375 CCTGCAGG CCTGCA^GG
TaqI 138 TCGA T^CGA
TthHB8I 138 TCGA T^CGA
Van91I 1851 CCAGAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AlwNI 749 CAGGTCCTG CAG[ACGT][ACGT][ACGT]^CTG
2138 CAGTCACTG
BanII 739 GAGCCC G[GA]GC[CT]^C
1461 GAGCTC
BbsI 16 GAAGAC GAAGAC(2/6)
1074 GAAGAC
BpuAI 16 GAAGAC GAAGAC(2/6)
1074 GAAGAC
BsaI 1633 GAGACC GGTCTC(1/5)
1678 GAGACC
BseRI 1191 CTCCTC GAGGAG(10/8)
1369 GAGGAG
BstYI 1064 AGATCC [GA]^GATC[CT]
2373 GGATCC
CfoI 129 GCGC GCG^C
239 GCGC
EcoT14I 821 CCTTGG C^C[AT][AT]GG
1919 CCTTGG
HapII 591 CCGG C^CGG
2218 CCGG
HhaI 129 GCGC GCG^C
239 GCGC
HinP1I 129 GCGC G^CGC
239 GCGC
HpaII 591 CCGG C^CGG
2218 CCGG
MflI 1064 AGATCC [GA]^GATC[CT]
2373 GGATCC
MspI 591 CCGG C^CGG
2218 CCGG
StyI 821 CCTTGG C^C[AT][AT]GG
1919 CCTTGG
XhoII 1064 AGATCC [GA]^GATC[CT]
2373 GGATCC
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# 3 OR MORE CUT ENZYMES ( 70 enzymes) #
#########################################
AciI AcsI AluI AlwI ApoI
Asp700I AspHI AvaII BbvI BfaI
BmyI BpmI BsaJI BsgI BsiHKAI
BsiYI BslI BsmAI Bsp1286I BspMI
BsrI BssKI BstF5I BstNI Cac8I
Cfr13I DdeI DpnII DraII EarI
EcoO109I EcoRII Fnu4HI FokI HaeIII
HinfI HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MnlI
MseI MspA1I MvaI MwoI NdeII
NlaIII NlaIV NspI PleI PpuMI
PstI PvuII Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI XmnI