Restriction map for
pF1KSDA0665
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# NO CUT ENZYMES ( 89 enzymes) #
##################################
AccIII AclI AcsI AflII AflIII
Aor51HI ApoI AscI AseI AsnI
Asp700I AviII AvrII BbrPI BciVI
BfaI BfrI BglII BlnI BlpI
Bpu1102I BsaAI BsaBI BseAI BsiWI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrBI BsrGI Bst1107I BstBI
BstEII BstPI BstZ17I CelII ClaI
CpoI DraI DraIII Eco47III EcoO65I
EcoRI EcoRV EcoT22I FspI HindIII
HpaI MaeI MamI MfeI MluI
MroI MunI NdeI NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflMI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SexAI SfuI SnaBI SpeI
SphI SspBI SspI SwaI Tsp509I
Van91I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AatII 475 GACGTC GACGT^C
Acc65I 1301 GGTACC G^GTACC
AgeI 1447 ACCGGT A^CCGGT
AocI 1467 CCTGAGG CC^T[ACGT]AGG
Asp718I 1301 GGTACC G^GTACC
AspI 886 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 738 GGATCC G^GATCC
BbeI 2083 GGCGCC GGCGC^C
BclI 758 TGATCA T^GATCA
BsiEI 1043 CGGCCG CG[GA][CT]^CG
BsmBI 2142 CGTCTC CGTCTC(1/5)
BsrDI 1383 CATTGC GCAATG(2/0)
BssHII 419 GCGCGC G^CGCGC
BssSI 1816 CACGAG CACGAG(-5/-1)
BstXI 983 CCAGCACCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1467 CCTGAGG CC^T[ACGT]AGG
EagI 1043 CGGCCG C^GGCCG
EclXI 1043 CGGCCG C^GGCCG
Eco52I 1043 CGGCCG C^GGCCG
Eco81I 1467 CCTGAGG CC^T[ACGT]AGG
EcoNI 1467 CCTGAGGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 758 TGATCA T^GATCA
FseI 218 GGCCGGCC GGCCGG^CC
HincII 472 GTCGAC GT[CT]^[GA]AC
HindII 472 GTCGAC GT[CT]^[GA]AC
KasI 2083 GGCGCC G^GCGCC
KpnI 1301 GGTACC GGTAC^C
KspI 286 CCGCGG CCGC^GG
MaeII 476 ACGT A^CGT
MseI 724 TTAA T^TAA
NarI 2083 GGCGCC GG^CGCC
PflFI 886 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 1447 ACCGGT A^CCGGT
SacII 286 CCGCGG CCGC^GG
SalI 472 GTCGAC G^TCGAC
ScaI 731 AGTACT AGT^ACT
SfoI 2083 GGCGCC GGC^GCC
SgrAI 1446 CACCGGTG C[GA]^CCGG[CT]G
SmlI 1903 CTCAAG C^T[CT][GA]AG
StuI 1114 AGGCCT AGG^CCT
TfiI 2169 GATTC G^A[AT]TC
Tru9I 724 TTAA T^TAA
Tth111I 886 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
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# TWO CUT ENZYMES ( 18 enzymes) #
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AccI 472 GTCGAC GT^[AC][GT]AC
697 GTCTAC
AhdI 145 GACCCGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1566 GACCCGGCGTC
Alw44I 994 GTGCAC G^TGCAC
1492 GTGCAC
ApaLI 994 GTGCAC G^TGCAC
1492 GTGCAC
AspEI 145 GACCCGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1566 GACCCGGCGTC
BbvCI 558 CCTCAGC CCTCAGC(-5/-2)
2070 CCTCAGC
BcgI 1083 CGACCATGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1613 GCATTGAGATCG
BsaWI 1447 ACCGGT [AT]^CCGG[AT]
1673 TCCGGT
BsgI 1306 CTGCAC GTGCAG(16/14)
1680 CTGCAC
BstAPI 1024 GCAGACAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1485 GCAGCTGGTGC
BstYI 738 GGATCC [GA]^GATC[CT]
807 AGATCC
Eam1105I 145 GACCCGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1566 GACCCGGCGTC
MflI 738 GGATCC [GA]^GATC[CT]
807 AGATCC
SapI 1608 GAAGAGC GCTCTTC(1/4)
1774 GAAGAGC
SbfI 1950 CCTGCAGG CCTGCA^GG
2199 CCTGCAGG
SfiI 9 GGCCCCGCCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
68 GGCCGGACGGGCC
Sse8387I 1950 CCTGCAGG CCTGCA^GG
2199 CCTGCAGG
XhoII 738 GGATCC [GA]^GATC[CT]
807 AGATCC
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# 3 OR MORE CUT ENZYMES ( 108 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI ApaI AspHI AvaI
AvaII BalI BanI BanII BbsI
BbvI BcnI BglI BmyI BpmI
BpuAI BsaHI BsaI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrFI BsrI
BssKI BstF5I BstNI BstUI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DrdI DsaI EaeI EarI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeIII MboI
MboII MluNI MnlI MscI MslI
MspA1I MspI MvaI MvnI MwoI
NaeI NciI NcoI NdeII NgoMIV
NlaIII NlaIV PleI PpuMI PstI
PvuII RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI SmaI StyI
TaqI TseI Tsp45I TspRI TthHB8I
XcmI XmaCI XmaI