Restriction map for
pF1KSDA0666
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# NO CUT ENZYMES ( 99 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AflII AgeI AhdI Alw44I
Aor51HI ApaLI AscI Asp718I AspEI
AspI AviII AvrII BamHI BbrPI
BcgI BfrI BglI BlnI BsaWI
BseAI BsiEI BsmBI BsmFI Bsp1407I
BspDI BspEI BspHI BsrBI BsrFI
BsrGI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstUI BstZ17I Cfr10I
ClaI CpoI EagI Eam1105I EclXI
Eco47III Eco52I EcoO65I FseI FspI
HgaI HincII HindII HpaI KpnI
KspI MfeI MluI MroI MunI
MvnI NaeI NgoMIV NheI NotI
NruI NspV PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI Psp1406I
PvuI RcaI RsrII SacII SalI
SapI SbfI SfiI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspBI
StuI SwaI Tth111I XhoI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AcyI 682 GGCGCC G[GA]^CG[CT]C
AflIII 2177 ACATGT A^C[GA][CT]GT
AocI 1745 CCTTAGG CC^T[ACGT]AGG
ApaI 1612 GGGCCC GGGCC^C
BalI 2261 TGGCCA TGG^CCA
BbeI 682 GGCGCC GGCGC^C
BbvCI 1810 CCTCAGC CCTCAGC(-5/-2)
BciVI 479 GTATCC GTATCC(6/5)
BsaAI 78 CACGTA [CT]AC^GT[GA]
BsaBI 1233 GATAGTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 682 GGCGCC G[GA]^CG[CT]C
BsiWI 1305 CGTACG C^GTACG
BspLU11I 2177 ACATGT A^CATGT
BspMI 1518 GCAGGT ACCTGC(4/8)
BsrDI 131 CATTGC GCAATG(2/0)
BstXI 1271 CCACACCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1745 CCTTAGG CC^T[ACGT]AGG
DraI 408 TTTAAA TTT^AAA
DraIII 1115 CACATAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 513 GACCTCAGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1745 CCTTAGG CC^T[ACGT]AGG
EcoRV 2508 GATATC GAT^ATC
EcoT22I 2492 ATGCAT ATGCA^T
HaeII 682 GGCGCC [GA]GCGC^[CT]
Hin1I 682 GGCGCC G[GA]^CG[CT]C
HindIII 1124 AAGCTT A^AGCTT
KasI 682 GGCGCC G^GCGCC
MamI 1233 GATAGTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 2261 TGGCCA TGG^CCA
MscI 2261 TGGCCA TGG^CCA
MslI 2583 CACTATTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 233 CAGCTG C[AC]G^C[GT]G
NarI 682 GGCGCC GG^CGCC
NdeI 2495 CATATG CA^TATG
NsiI 2492 ATGCAT ATGCA^T
NspI 2177 ACATGT [GA]CATG^[CT]
PacI 778 TTAATTAA TTAAT^TAA
PflMI 1762 CCACCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 721 CTGCAG CTGCA^G
PvuII 233 CAGCTG CAG^CTG
ScaI 1199 AGTACT AGT^ACT
SexAI 1671 ACCAGGT A^CC[AT]GGT
SfoI 682 GGCGCC GGC^GCC
SmaI 700 CCCGGG CCC^GGG
SspI 1291 AATATT AAT^ATT
Tsp45I 2216 GTGAC ^GT[GC]AC
Van91I 1762 CCACCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 1917 TCTAGA T^CTAGA
XmaCI 700 CCCGGG C^CCGGG
XmaI 700 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 17 enzymes) #
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AlwI 1636 GGATC GGATC(4/5)
2253 GGATC
AvaI 700 CCCGGG C^[CT]CG[GA]G
1855 CCCGAG
BanI 682 GGCGCC G^G[CT][GA]CC
2422 GGTGCC
BglII 1920 AGATCT A^GATCT
2163 AGATCT
BsaI 511 GAGACC GGTCTC(1/5)
3210 GAGACC
BsmI 2490 GAATGC GAATGC(1/-1)
1805 GCATTC
BsoBI 700 CCCGGG C^[CT]CG[GA]G
1855 CCCGAG
BstAPI 723 GCAGGCCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2313 GCAAAGGTTGC
CfoI 109 GCGC GCG^C
683 GCGC
EaeI 662 TGGCCG [CT]^GGCC[GA]
2261 TGGCCA
EcoRI 310 GAATTC G^AATTC
935 GAATTC
FauI 764 CCCGC CCCGC(4/6)
1131 CCCGC
HhaI 109 GCGC GCG^C
683 GCGC
HinP1I 109 GCGC G^CGC
683 GCGC
HphI 2058 GGTGA GGTGA(8/7)
66 TCACC
PpuMI 760 AGGACCC [GA]G^G[AT]CC[CT]
1259 AGGACCC
XcmI 137 CCATGCCCCCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2171 CCAAGGACATGTTGG
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# 3 OR MORE CUT ENZYMES ( 92 enzymes) #
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AciI AcsI AfaI AluI AlwNI
ApoI AseI AsnI Asp700I AspHI
AvaII BanII BbsI BbvI BclI
BcnI BfaI BlpI BmyI BpmI
Bpu1102I BpuAI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI Bsp1286I
BsrI BssKI BstF5I BstNI BstYI
Cac8I CelII Cfr13I DdeI DpnII
DraII DsaI EarI EcoNI EcoO109I
EcoRII EcoT14I FbaI Fnu4HI FokI
HaeIII HapII HinfI HpaII ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MspI
MvaI MwoI NciI NcoI NdeII
NlaIII NlaIV PleI PshBI RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TaqI TfiI
Tru9I TseI Tsp509I TspRI TthHB8I
XhoII XmnI