################################## # NO CUT ENZYMES ( 78 enzymes) # ################################## AccIII AclI AflIII AgeI Alw44I Aor51HI ApaLI AscI AseI AsnI AviII BamHI BbeI BbrPI BsaWI BseAI BsiWI BsmBI BspDI BspEI BspLU11I BsrDI BssHII BssSI BstBI BstEII BstPI ClaI CpoI DraI DraIII EagI EclXI Eco47III Eco52I EcoO65I EcoRI EcoT22I FseI FspI HindIII HpaI KasI KspI MluI MroI NaeI NarI NdeI NgoMIV NheI NotI NsiI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI RsrII SacII SalI SbfI SfiI SfoI SfuI SgrAI SnaBI SpeI Sse8387I SspI SwaI XbaI XhoI ################################### # ONE CUT ENZYMES ( 57 enzymes) # ################################### AatII 1441 GACGTC GACGT^C Acc65I 210 GGTACC G^GTACC AccII 2794 CGCG CG^CG AcsI 1262 GAATTT [GA]^AATT[CT] AcyI 1441 GACGTC G[GA]^CG[CT]C AflII 1819 CTTAAG C^TTAAG AhdI 2243 GACAGCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC ApaI 2344 GGGCCC GGGCC^C ApoI 1262 GAATTT [GA]^AATT[CT] Asp700I 2832 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 210 GGTACC G^GTACC AspEI 2243 GACAGCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 1519 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 959 CCCGGG C^[CT]CG[GA]G AvrII 1611 CCTAGG C^CTAGG BcgI 2784 GCAGATCCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 2769 GGATAC GTATCC(6/5) BfrI 1819 CTTAAG C^TTAAG BglI 382 GCCTCACTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlnI 1611 CCTAGG C^CTAGG BsaAI 823 TACGTG [CT]AC^GT[GA] BsaBI 1491 GATCAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 1441 GACGTC G[GA]^CG[CT]C BsiEI 2583 CGGTCG CG[GA][CT]^CG BsmI 792 GAATGC GAATGC(1/-1) BsoBI 959 CCCGGG C^[CT]CG[GA]G Bsp1407I 2201 TGTACA T^GTACA BsrBI 4 GAGCGG CCGCTC(-3/-3) BsrFI 1157 ACCGGC [GA]^CCGG[CT] BsrGI 2201 TGTACA T^GTACA Bst1107I 1141 GTATAC GTA^TAC BstUI 2794 CGCG CG^CG BstZ17I 1141 GTATAC GTA^TAC Cfr10I 1157 ACCGGC [GA]^CCGG[CT] DrdI 1564 GACCACCCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eam1105I 2243 GACAGCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoNI 2388 CCTGGCACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRV 2659 GATATC GAT^ATC Hin1I 1441 GACGTC G[GA]^CG[CT]C KpnI 210 GGTACC GGTAC^C MamI 1491 GATCAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 2288 CAATTG C^AATTG MunI 2288 CAATTG C^AATTG MvnI 2794 CGCG CG^CG NruI 2793 TCGCGA TCG^CGA PflFI 1519 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC PpuMI 2016 GGGACCC [GA]G^G[AT]CC[CT] PshAI 1954 GACATCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC ScaI 224 AGTACT AGT^ACT SexAI 2730 ACCTGGT A^CC[AT]GGT SmaI 959 CCCGGG CCC^GGG SphI 1605 GCATGC GCATG^C SspBI 2201 TGTACA T^GTACA Tth111I 1519 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC XmaCI 959 CCCGGG C^CCGGG XmaI 959 CCCGGG C^CCGGG XmnI 2832 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AccI 1141 GTATAC GT^[AC][GT]AC 1165 GTCTAC AocI 520 CCTGAGG CC^T[ACGT]AGG 2485 CCTGAGG BbvCI 2751 CCTCAGC CCTCAGC(-5/-2) 454 GCTGAGG BclI 1577 TGATCA T^GATCA 1974 TGATCA BfaI 490 CTAG C^TAG 1612 CTAG BglII 254 AGATCT A^GATCT 1658 AGATCT BlpI 1326 GCTCAGC GC^T[ACGT]AGC 2073 GCTGAGC Bpu1102I 1326 GCTCAGC GC^T[ACGT]AGC 2073 GCTGAGC BstAPI 189 GCAGCTACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 882 GCAGGCAGTGC Bsu36I 520 CCTGAGG CC^T[ACGT]AGG 2485 CCTGAGG CelII 1326 GCTCAGC GC^T[ACGT]AGC 2073 GCTGAGC Eco81I 520 CCTGAGG CC^T[ACGT]AGG 2485 CCTGAGG FbaI 1577 TGATCA T^GATCA 1974 TGATCA HgaI 2142 GACGC GACGC(5/10) 654 GCGTC HincII 1009 GTCAAC GT[CT]^[GA]AC 1444 GTCAAC HindII 1009 GTCAAC GT[CT]^[GA]AC 1444 GTCAAC MaeI 490 CTAG C^TAG 1612 CTAG MseI 1820 TTAA T^TAA 2138 TTAA SacI 760 GAGCTC GAGCT^C 1541 GAGCTC TaqI 767 TCGA T^CGA 2241 TCGA TfiI 1229 GATTC G^A[AT]TC 2371 GATTC Tru9I 1820 TTAA T^TAA 2138 TTAA Tsp509I 1263 AATT ^AATT 2289 AATT TthHB8I 767 TCGA T^CGA 2241 TCGA XcmI 575 CCAGCCTGGCGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 2021 CCAACCCCGTGGTGG ######################################### # 3 OR MORE CUT ENZYMES ( 98 enzymes) # ######################################### AciI AfaI AluI AlwI AlwNI AspHI AvaII BalI BanI BanII BbsI BbvI BcnI BmyI BpmI BpuAI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BspHI BspMI BsrI BssKI BstF5I BstNI BstXI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MslI MspA1I MspI MvaI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PflMI PleI PstI PvuII RcaI RsaI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StuI StyI TseI Tsp45I TspRI Van91I XhoII