Restriction map for
pF1KSDA0741
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# NO CUT ENZYMES ( 102 enzymes) #
##################################
AatII AccI AccIII AclI AflII
AgeI AhdI Alw44I AocI Aor51HI
ApaLI AscI AspEI AspI AviII
AvrII BamHI BbeI BbrPI BbvCI
BcgI BciVI BfrI BglI BglII
BlnI BsaAI BsaI BsaWI BseAI
BsiEI BsiWI BsmBI BspDI BspEI
BspHI BsrBI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstXI
BstZ17I Bsu36I ClaI CpoI DsaI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I FseI FspI
HaeII KasI KspI MluI MroI
NarI NcoI NdeI NheI NotI
NruI NspV PacI PflFI PflMI
PinAI PmaCI PmeI PmlI PshAI
Psp1406I PvuI RcaI RsrII SacII
SalI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspI SwaI Tth111I
Van91I XcmI
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# ONE CUT ENZYMES ( 62 enzymes) #
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Acc65I 2460 GGTACC G^GTACC
AccII 712 CGCG CG^CG
AcyI 658 GACGCC G[GA]^CG[CT]C
AflIII 1962 ACATGT A^C[GA][CT]GT
AlwNI 1766 CAGATTCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 2683 GGGCCC GGGCC^C
AseI 2032 ATTAAT AT^TAAT
AsnI 2032 ATTAAT AT^TAAT
Asp700I 536 GAATAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 2460 GGTACC G^GTACC
BalI 1526 TGGCCA TGG^CCA
BclI 2630 TGATCA T^GATCA
BlpI 1045 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 1045 GCTAAGC GC^T[ACGT]AGC
BsaHI 658 GACGCC G[GA]^CG[CT]C
Bsp1407I 1965 TGTACA T^GTACA
BspLU11I 1962 ACATGT A^CATGT
BsrDI 2163 CATTGC GCAATG(2/0)
BsrFI 3560 GCCGGC [GA]^CCGG[CT]
BsrGI 1965 TGTACA T^GTACA
BstUI 712 CGCG CG^CG
CelII 1045 GCTAAGC GC^T[ACGT]AGC
CfoI 711 GCGC GCG^C
Cfr10I 3560 GCCGGC [GA]^CCGG[CT]
DraI 2325 TTTAAA TTT^AAA
DraIII 2552 CACACTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 2208 GACAATTGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 1526 TGGCCA [CT]^GGCC[GA]
EcoRI 268 GAATTC G^AATTC
EcoRV 169 GATATC GAT^ATC
EcoT22I 2188 ATGCAT ATGCA^T
FauI 2441 CCCGC CCCGC(4/6)
FbaI 2630 TGATCA T^GATCA
HgaI 658 GACGC GACGC(5/10)
HhaI 711 GCGC GCG^C
Hin1I 658 GACGCC G[GA]^CG[CT]C
HinP1I 711 GCGC G^CGC
HindIII 1048 AAGCTT A^AGCTT
HpaI 2520 GTTAAC GTT^AAC
KpnI 2460 GGTACC GGTAC^C
MaeII 1092 ACGT A^CGT
MfeI 2210 CAATTG C^AATTG
MluNI 1526 TGGCCA TGG^CCA
MscI 1526 TGGCCA TGG^CCA
MspA1I 908 CAGCTG C[AC]G^C[GT]G
MunI 2210 CAATTG C^AATTG
MvnI 712 CGCG CG^CG
NaeI 3560 GCCGGC GCC^GGC
NgoMIV 3560 GCCGGC G^CCGGC
NsiI 2188 ATGCAT ATGCA^T
PaeR7I 3311 CTCGAG C^TCGAG
PshBI 2032 ATTAAT AT^TAAT
PvuII 908 CAGCTG CAG^CTG
SacI 1761 GAGCTC GAGCT^C
SapI 2560 GAAGAGC GCTCTTC(1/4)
SmaI 2598 CCCGGG CCC^GGG
SspBI 1965 TGTACA T^GTACA
StuI 2706 AGGCCT AGG^CCT
XhoI 3311 CTCGAG C^TCGAG
XmaCI 2598 CCCGGG C^CCGGG
XmaI 2598 CCCGGG C^CCGGG
XmnI 536 GAATAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AciI 2442 CCGC CCGC(-3/-1)
750 GCGG
AspHI 1761 GAGCTC G[AT]GC[AT]^C
2570 GAGCAC
AvaI 2598 CCCGGG C^[CT]CG[GA]G
3311 CTCGAG
AvaII 625 GGTCC G^G[AT]CC
1003 GGACC
BanI 2460 GGTACC G^G[CT][GA]CC
2608 GGCACC
BcnI 2598 CCCGG CC^[GC]GG
2599 CCGGG
BsaBI 1786 GATGATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3279 GATAAAAATC
BsgI 2471 CTGCAC GTGCAG(16/14)
3173 GTGCAG
BsiHKAI 1761 GAGCTC G[AT]GC[AT]^C
2570 GAGCAC
BsmI 2090 GAATGC GAATGC(1/-1)
2409 GAATGC
BsoBI 2598 CCCGGG C^[CT]CG[GA]G
3311 CTCGAG
BspMI 1981 GCAGGT ACCTGC(4/8)
3346 GCAGGT
BstYI 2436 AGATCC [GA]^GATC[CT]
3315 AGATCC
Cac8I 2367 GCGAGC GC[ACGT]^[ACGT]GC
3560 GCCGGC
EcoT14I 38 CCAAGG C^C[AT][AT]GG
62 CCTTGG
HapII 2599 CCGG C^CGG
3561 CCGG
HincII 2520 GTTAAC GT[CT]^[GA]AC
3397 GTTGAC
HindII 2520 GTTAAC GT[CT]^[GA]AC
3397 GTTGAC
HpaII 2599 CCGG C^CGG
3561 CCGG
MamI 1786 GATGATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3279 GATAAAAATC
MflI 2436 AGATCC [GA]^GATC[CT]
3315 AGATCC
MspI 2599 CCGG C^CGG
3561 CCGG
NciI 2598 CCCGG CC^[GC]GG
2599 CCGGG
NspI 1914 GCATGT [GA]CATG^[CT]
1962 ACATGT
PpuMI 624 AGGTCCT [GA]G^G[AT]CC[CT]
1002 AGGACCT
PstI 68 CTGCAG CTGCA^G
911 CTGCAG
SmlI 206 CTCAAG C^T[CT][GA]AG
3311 CTCGAG
StyI 38 CCAAGG C^C[AT][AT]GG
62 CCTTGG
XbaI 586 TCTAGA T^CTAGA
1941 TCTAGA
XhoII 2436 AGATCC [GA]^GATC[CT]
3315 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 64 enzymes) #
#########################################
AcsI AfaI AluI AlwI ApoI
BanII BbsI BbvI BfaI BmyI
BpmI BpuAI BsaJI BseRI BsiYI
BslI BsmAI BsmFI Bsp1286I BsrI
BssKI BstF5I BstNI Cfr13I DdeI
DpnII DraII EarI EcoO109I EcoRII
Fnu4HI FokI HaeIII HinfI HphI
ItaI Ksp632I MaeI MaeIII MboI
MboII MnlI MseI MslI MvaI
MwoI NdeII NlaIII NlaIV PleI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I