Restriction map for
pF1KSDA0752
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# NO CUT ENZYMES ( 162 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AciI AclI AcsI AcyI AflII
AflIII AgeI AhdI Alw44I AlwI
AocI Aor51HI ApaI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AviII AvrII BalI
BamHI BbeI BbrPI BbvCI BcgI
BciVI BcnI BfrI BglII BlnI
BlpI Bpu1102I BsaAI BsaBI BsaHI
BsaI BsaWI BseAI BsiEI BsiWI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrBI BsrDI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstUI
BstXI BstYI BstZ17I Bsu36I CelII
CfoI ClaI CpoI DraI DrdI
DsaI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoRI EcoRV
EcoT22I FauI FseI FspI HaeII
HhaI Hin1I HinP1I HincII HindII
HindIII HpaI KasI KpnI KspI
MamI MflI MluI MluNI MroI
MscI MspA1I MvnI NaeI NarI
NciI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
PvuII RcaI RsrII SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI TaqI TfiI
Tth111I TthHB8I XhoI XhoII XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AspHI 61 GAGCTC G[AT]GC[AT]^C
AvaI 172 CCCGAG C^[CT]CG[GA]G
BanI 316 GGCACC G^G[CT][GA]CC
BanII 61 GAGCTC G[GA]GC[CT]^C
BbvI 758 GCAGC GCAGC(8/12)
BclI 437 TGATCA T^GATCA
BfaI 241 CTAG C^TAG
BmyI 61 GAGCTC G[AGT]GC[ACT]^C
BsgI 791 CTGCAC GTGCAG(16/14)
BsiHKAI 61 GAGCTC G[AT]GC[AT]^C
BsmBI 187 GAGACG CGTCTC(1/5)
BsoBI 172 CCCGAG C^[CT]CG[GA]G
Bsp1286I 61 GAGCTC G[AGT]GC[ACT]^C
BsrFI 887 GCCGGT [GA]^CCGG[CT]
Cfr10I 887 GCCGGT [GA]^CCGG[CT]
EaeI 425 TGGCCG [CT]^GGCC[GA]
EarI 829 GAAGAG CTCTTC(1/4)
FbaI 437 TGATCA T^GATCA
Fnu4HI 758 GCAGC GC^[ACGT]GC
HapII 888 CCGG C^CGG
HgaI 310 GACGC GACGC(5/10)
HpaII 888 CCGG C^CGG
ItaI 758 GCAGC GC^[ACGT]GC
Ksp632I 829 GAAGAG CTCTTC(1/4)
MaeI 241 CTAG C^TAG
MfeI 733 CAATTG C^AATTG
MseI 920 TTAA T^TAA
MslI 27 CATTGAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 888 CCGG C^CGG
MunI 733 CAATTG C^AATTG
PflMI 520 CCACAACGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 797 CTGCAG CTGCA^G
SacI 61 GAGCTC GAGCT^C
SfcI 797 CTGCAG C^T[GA][CT]AG
Tru9I 920 TTAA T^TAA
TseI 758 GCAGC G^C[AT]GC
Tsp509I 734 AATT ^AATT
Van91I 520 CCACAACGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 240 TCTAGA T^CTAGA
XcmI 326 CCAACGGTGACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AfaI 90 GTAC GT^AC
448 GTAC
AluI 62 AGCT AG^CT
458 AGCT
BglI 616 GCCACGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
718 GCCACACTGGC
BsiYI 489 CCCAGAGGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
520 CCACAACGTGG
BslI 489 CCCAGAGGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
520 CCACAACGTGG
BsmFI 410 GGGAC GGGAC(10/14)
911 GGGAC
BstEII 331 GGTGACC G^GT[ACGT]ACC
529 GGTGACC
BstPI 331 GGTGACC G^GT[ACGT]ACC
529 GGTGACC
DpnII 235 GATC ^GATC
438 GATC
DraII 410 GGGACCC [GA]G^G[ACGT]CC[CT]
485 AGGACCC
DraIII 125 CACAACGTG CAC[ACGT][ACGT][ACGT]^GTG
521 CACAACGTG
EcoO109I 410 GGGACCC [GA]G^G[ACGT]CC[CT]
485 AGGACCC
EcoO65I 331 GGTGACC G^GT[ACGT]ACC
529 GGTGACC
HaeIII 359 GGCC GG^CC
426 GGCC
HinfI 285 GAGTC G^A[ACGT]TC
742 GACTC
MboI 235 GATC ^GATC
438 GATC
NdeII 235 GATC ^GATC
438 GATC
NspI 552 GCATGT [GA]CATG^[CT]
654 GCATGT
PleI 742 GACTC GAGTC(4/5)
285 GAGTC
PpuMI 410 GGGACCC [GA]G^G[AT]CC[CT]
485 AGGACCC
RsaI 90 GTAC GT^AC
448 GTAC
Sau3AI 235 GATC ^GATC
438 GATC
#########################################
# 3 OR MORE CUT ENZYMES ( 34 enzymes) #
#########################################
AlwNI AvaII BbsI BpmI BpuAI
BsaJI BseRI BsmAI BspMI BsrI
BssKI BstF5I BstNI Cac8I Cfr13I
DdeI EcoRII EcoT14I FokI HphI
MaeII MaeIII MboII MnlI MvaI
MwoI NlaIII NlaIV Sau96I ScrFI
SfaNI StyI Tsp45I TspRI