Restriction map for
pF1KSDA0763
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# NO CUT ENZYMES ( 73 enzymes) #
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AatII Acc65I AccI AclI AflII
AgeI AscI AseI AsnI Asp718I
AvrII BbrPI BbsI BciVI BclI
BfrI BlnI BpuAI BsaAI BsiWI
BsmBI BsmI BspHI BssHII Bst1107I
BstBI BstZ17I CpoI DraI DraIII
EcoRI EcoT22I FbaI FseI HincII
HindII HindIII HpaI KpnI KspI
MfeI MluI MunI NdeI NheI
NotI NruI NsiI NspV PacI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I RcaI RsrII SacII
SalI SbfI SexAI SfuI SgrAI
SnaBI SpeI Sse8387I SspI StuI
SwaI TfiI XbaI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AccII 455 CGCG CG^CG
AccIII 1571 TCCGGA T^CCGGA
AflIII 1628 ACATGT A^C[GA][CT]GT
Alw44I 193 GTGCAC G^TGCAC
AocI 1455 CCTCAGG CC^T[ACGT]AGG
ApaLI 193 GTGCAC G^TGCAC
AspI 1405 GACTGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 104 TGCGCA TGC^GCA
BalI 980 TGGCCA TGG^CCA
BcgI 2358 CGACAGCTATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 954 GCCCCCCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 2000 AGATCT A^GATCT
BsaBI 1734 GATGGGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 222 GGTCTC GGTCTC(1/5)
BseAI 1571 TCCGGA T^CCGGA
BspDI 553 ATCGAT AT^CGAT
BspEI 1571 TCCGGA T^CCGGA
BspLU11I 1628 ACATGT A^CATGT
BssSI 702 CACGAG CACGAG(-5/-1)
BstUI 455 CGCG CG^CG
Bsu36I 1455 CCTCAGG CC^T[ACGT]AGG
ClaI 553 ATCGAT AT^CGAT
DrdI 1294 GACACGCCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 1436 CCATGG C^C[GA][CT]GG
EagI 604 CGGCCG C^GGCCG
EclXI 604 CGGCCG C^GGCCG
Eco52I 604 CGGCCG C^GGCCG
Eco81I 1455 CCTCAGG CC^T[ACGT]AGG
EcoRV 2137 GATATC GAT^ATC
FspI 104 TGCGCA TGC^GCA
MamI 1734 GATGGGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 980 TGGCCA TGG^CCA
MroI 1571 TCCGGA T^CCGGA
MscI 980 TGGCCA TGG^CCA
MseI 2149 TTAA T^TAA
MvnI 455 CGCG CG^CG
NcoI 1436 CCATGG C^CATGG
PaeR7I 2260 CTCGAG C^TCGAG
PflFI 1405 GACTGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 2226 CCAAGAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuI 1835 CGATCG CGAT^CG
SacI 2257 GAGCTC GAGCT^C
SapI 2076 GCTCTTC GCTCTTC(1/4)
ScaI 2090 AGTACT AGT^ACT
SfiI 953 GGCCCCCCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SphI 2063 GCATGC GCATG^C
Tru9I 2149 TTAA T^TAA
Tth111I 1405 GACTGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 2226 CCAAGAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 2260 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 30 enzymes) #
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AhdI 601 GACCGGCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1783 GACCATGTGTC
AlwNI 742 CAGCGGCTG CAG[ACGT][ACGT][ACGT]^CTG
2068 CAGGTCCTG
Aor51HI 98 AGCGCT AGC^GCT
1532 AGCGCT
ApaI 458 GGGCCC GGGCC^C
665 GGGCCC
AspEI 601 GACCGGCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1783 GACCATGTGTC
BamHI 1751 GGATCC G^GATCC
1840 GGATCC
BbeI 23 GGCGCC GGCGC^C
907 GGCGCC
BlpI 992 GCTCAGC GC^T[ACGT]AGC
1131 GCTCAGC
Bpu1102I 992 GCTCAGC GC^T[ACGT]AGC
1131 GCTCAGC
BsaWI 1571 TCCGGA [AT]^CCGG[AT]
2180 ACCGGA
BsiEI 604 CGGCCG CG[GA][CT]^CG
1835 CGATCG
BsmAI 223 GTCTC GTCTC(1/5)
306 GTCTC
Bsp1407I 548 TGTACA T^GTACA
1631 TGTACA
BsrDI 2214 CATTGC GCAATG(2/0)
2471 GCAATG
BsrGI 548 TGTACA T^GTACA
1631 TGTACA
BstAPI 741 GCAGCGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2067 GCAGGTCCTGC
CelII 992 GCTCAGC GC^T[ACGT]AGC
1131 GCTCAGC
Eam1105I 601 GACCGGCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1783 GACCATGTGTC
EarI 1234 CTCTTC CTCTTC(1/4)
2077 CTCTTC
Eco47III 98 AGCGCT AGC^GCT
1532 AGCGCT
KasI 23 GGCGCC G^GCGCC
907 GGCGCC
Ksp632I 1234 CTCTTC CTCTTC(1/4)
2077 CTCTTC
NaeI 1343 GCCGGC GCC^GGC
1904 GCCGGC
NarI 23 GGCGCC GG^CGCC
907 GGCGCC
NgoMIV 1343 GCCGGC G^CCGGC
1904 GCCGGC
PpuMI 2069 AGGTCCT [GA]G^G[AT]CC[CT]
2414 GGGACCT
PvuII 247 CAGCTG CAG^CTG
1167 CAGCTG
SfoI 23 GGCGCC GGC^GCC
907 GGCGCC
SspBI 548 TGTACA T^GTACA
1631 TGTACA
XcmI 338 CCATCACCGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1586 CCATTTTCATCCTGG
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# 3 OR MORE CUT ENZYMES ( 105 enzymes) #
#########################################
AciI AcsI AcyI AfaI AluI
AlwI ApoI Asp700I AspHI AvaI
AvaII BanI BanII BbvCI BbvI
BcnI BfaI BmyI BpmI BsaHI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmFI BsoBI Bsp1286I BspMI
BsrBI BsrFI BsrI BssKI BstEII
BstF5I BstNI BstPI BstXI BstYI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII EaeI EcoNI EcoO109I
EcoO65I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI MaeI MaeII MaeIII
MboI MboII MflI MnlI MslI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV NspI PleI
PstI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmaI SmlI StyI
TaqI TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII XmaCI XmaI XmnI