Restriction map for
pF1KSDA0764
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# NO CUT ENZYMES ( 145 enzymes) #
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Acc65I AccI AccII AccIII AclI
AcsI AfaI AflIII AgeI AhdI
Alw44I AocI ApaI ApaLI ApoI
AscI AseI AsnI Asp718I AspEI
AspI AvaI AviII AvrII BamHI
BanI BbeI BbrPI BciVI BclI
BfaI BglI BglII BlnI BlpI
Bpu1102I BsaAI BsaBI BsaWI BseAI
BsiEI BsiWI BsmBI BsmI BsoBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrBI BsrFI BsrGI BssHII Bst1107I
BstBI BstUI BstZ17I Bsu36I CelII
Cfr10I ClaI CpoI DraI DraIII
DrdI EagI Eam1105I EclXI Eco52I
Eco81I EcoRI EcoRV EcoT14I EcoT22I
FbaI FseI FspI HincII HindII
HpaI KasI KpnI KspI MaeI
MamI MfeI MluI MroI MslI
MunI MvnI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PstI PvuI RcaI
RsaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI StyI SwaI Tsp509I Tth111I
XbaI XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AatII 221 GACGTC GACGT^C
AcyI 221 GACGTC G[GA]^CG[CT]C
AflII 571 CTTAAG C^TTAAG
AlwI 384 GATCC GGATC(4/5)
AlwNI 898 CAGTCACTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 1214 AGCGCT AGC^GCT
Asp700I 298 GAACCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 682 GTGCTC G[AT]GC[AT]^C
BalI 485 TGGCCA TGG^CCA
BbvCI 125 CCTCAGC CCTCAGC(-5/-2)
BcgI 453 GCACTCCTGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 571 CTTAAG C^TTAAG
BsaHI 221 GACGTC G[GA]^CG[CT]C
BsiHKAI 682 GTGCTC G[AT]GC[AT]^C
BsmFI 621 GGGAC GGGAC(10/14)
BspMI 651 GCAGGT ACCTGC(4/8)
BsrDI 1055 GCAATG GCAATG(2/0)
BssSI 309 CTCGTG CACGAG(-5/-1)
BstAPI 1068 GCATGGTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 320 GGTCACC G^GT[ACGT]ACC
BstPI 320 GGTCACC G^GT[ACGT]ACC
BstYI 383 AGATCC [GA]^GATC[CT]
CfoI 1215 GCGC GCG^C
DraII 1192 GGGTCCT [GA]G^G[ACGT]CC[CT]
DsaI 76 CCACGG C^C[GA][CT]GG
EaeI 485 TGGCCA [CT]^GGCC[GA]
Eco47III 1214 AGCGCT AGC^GCT
EcoO109I 1192 GGGTCCT [GA]G^G[ACGT]CC[CT]
EcoO65I 320 GGTCACC G^GT[ACGT]ACC
FauI 505 GCGGG CCCGC(4/6)
HaeII 1214 AGCGCT [GA]GCGC^[CT]
HgaI 805 GACGC GACGC(5/10)
HhaI 1215 GCGC GCG^C
Hin1I 221 GACGTC G[GA]^CG[CT]C
HinP1I 1215 GCGC G^CGC
HindIII 962 AAGCTT A^AGCTT
MaeII 222 ACGT A^CGT
MflI 383 AGATCC [GA]^GATC[CT]
MluNI 485 TGGCCA TGG^CCA
MscI 485 TGGCCA TGG^CCA
MseI 572 TTAA T^TAA
MspA1I 447 CAGCTG C[AC]G^C[GT]G
NspI 734 ACATGC [GA]CATG^[CT]
PpuMI 1192 GGGTCCT [GA]G^G[AT]CC[CT]
PvuII 447 CAGCTG CAG^CTG
SfaNI 713 GCATC GCATC(5/9)
SfcI 739 CTACAG C^T[GA][CT]AG
Tru9I 572 TTAA T^TAA
XhoII 383 AGATCC [GA]^GATC[CT]
XmnI 298 GAACCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 23 enzymes) #
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AciI 711 CCGC CCGC(-3/-1)
505 GCGG
AvaII 603 GGACC G^G[AT]CC
1193 GGTCC
BanII 923 GGGCTC G[GA]GC[CT]^C
1137 GAGCCC
BbsI 224 GTCTTC GAAGAC(2/6)
1090 GTCTTC
BcnI 606 CCGGG CC^[GC]GG
614 CCCGG
BpuAI 224 GTCTTC GAAGAC(2/6)
1090 GTCTTC
BsaI 538 GAGACC GGTCTC(1/5)
893 GAGACC
BsgI 115 CTGCAC GTGCAG(16/14)
983 GTGCAG
BsrI 17 ACTGG ACTGG(1/-1)
897 CCAGT
BstXI 149 CCACTATGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
488 CCACAATCCTGG
Cac8I 449 GCTGGC GC[ACGT]^[ACGT]GC
505 GCGGGC
DpnII 384 GATC ^GATC
822 GATC
EcoNI 471 CCTCTACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1102 CCTATGTCAGG
MboI 384 GATC ^GATC
822 GATC
NciI 606 CCGGG CC^[GC]GG
614 CCCGG
NdeII 384 GATC ^GATC
822 GATC
PflMI 477 CCAGGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
660 CCATGAAGTGG
PleI 627 GACTC GAGTC(4/5)
1147 GACTC
Sau3AI 384 GATC ^GATC
822 GATC
SmlI 571 CTTAAG C^T[CT][GA]AG
1037 CTCAAG
TaqI 65 TCGA T^CGA
1094 TCGA
TthHB8I 65 TCGA T^CGA
1094 TCGA
Van91I 477 CCAGGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
660 CCATGAAGTGG
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# 3 OR MORE CUT ENZYMES ( 40 enzymes) #
#########################################
AluI BbvI BmyI BpmI BsaJI
BseRI BsiYI BslI BsmAI Bsp1286I
BssKI BstF5I BstNI Cfr13I DdeI
EarI EcoRII Fnu4HI FokI HaeIII
HapII HinfI HpaII HphI ItaI
Ksp632I MaeIII MboII MnlI MspI
MvaI MwoI NlaIII NlaIV Sau96I
ScrFI TfiI TseI Tsp45I TspRI