Restriction map for
pF1KSDA0769
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# NO CUT ENZYMES ( 122 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AgeI Alw44I Aor51HI
ApaLI AscI AseI AsnI Asp718I
AspI AviII AvrII BamHI BbeI
BbvCI BcgI BlnI BlpI Bpu1102I
BsaHI BsaWI BseAI BsiEI BsiWI
BsmBI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstUI BstZ17I CelII
CfoI Cfr10I ClaI CpoI DraI
DraII DraIII DrdI EagI EclXI
Eco47III Eco52I EcoNI EcoO109I EcoO65I
EcoRV EcoT22I FseI FspI HaeII
HgaI HhaI Hin1I HinP1I KasI
KpnI KspI MfeI MluI MroI
MunI MvnI NaeI NarI NgoMIV
NheI NotI NruI NsiI NspV
PacI PflFI PinAI PmeI PpuMI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI Sse8387I
SspBI StuI SwaI Tth111I XbaI
XmaCI XmaI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AcsI 1663 GAATTC [GA]^AATT[CT]
AflII 1230 CTTAAG C^TTAAG
AhdI 1462 GACAGTCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 1597 CCTGAGG CC^T[ACGT]AGG
ApaI 1097 GGGCCC GGGCC^C
ApoI 1663 GAATTC [GA]^AATT[CT]
AspEI 1462 GACAGTCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 103 CTCGAG C^[CT]CG[GA]G
BalI 1374 TGGCCA TGG^CCA
BanI 1225 GGCACC G^G[CT][GA]CC
BbsI 1345 GAAGAC GAAGAC(2/6)
BciVI 81 GTATCC GTATCC(6/5)
BclI 558 TGATCA T^GATCA
BfrI 1230 CTTAAG C^TTAAG
BglI 1217 GCCCTTCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1757 AGATCT A^GATCT
BpuAI 1345 GAAGAC GAAGAC(2/6)
BsaBI 1752 GATTCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI 619 GTGCAG GTGCAG(16/14)
BsoBI 103 CTCGAG C^[CT]CG[GA]G
BstAPI 481 GCAGCAAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 1597 CCTGAGG CC^T[ACGT]AGG
Eam1105I 1462 GACAGTCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1597 CCTGAGG CC^T[ACGT]AGG
EcoRI 1663 GAATTC G^AATTC
EcoT14I 1738 CCATGG C^C[AT][AT]GG
FauI 1100 CCCGC CCCGC(4/6)
FbaI 558 TGATCA T^GATCA
HindIII 192 AAGCTT A^AGCTT
HpaI 523 GTTAAC GTT^AAC
MamI 1752 GATTCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 1374 TGGCCA TGG^CCA
MscI 1374 TGGCCA TGG^CCA
MspA1I 682 CAGCTG C[AC]G^C[GT]G
NcoI 1738 CCATGG C^CATGG
NdeI 1952 CATATG CA^TATG
PaeR7I 103 CTCGAG C^TCGAG
PflMI 1065 CCAAGTAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 1906 GAGTC GAGTC(4/5)
PvuII 682 CAGCTG CAG^CTG
SacI 1851 GAGCTC GAGCT^C
SphI 1436 GCATGC GCATG^C
SspI 961 AATATT AAT^ATT
StyI 1738 CCATGG C^C[AT][AT]GG
Van91I 1065 CCAAGTAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1635 CCAAGATGATGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 103 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AlwI 240 GATCC GGATC(4/5)
498 GGATC
AlwNI 773 CAGAAACTG CAG[ACGT][ACGT][ACGT]^CTG
1444 CAGTCCCTG
Asp700I 624 GAACACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
958 GAAAATATTC
AspHI 793 GAGCAC G[AT]GC[AT]^C
1851 GAGCTC
BanII 1097 GGGCCC G[GA]GC[CT]^C
1851 GAGCTC
BbrPI 830 CACGTG CAC^GTG
839 CACGTG
BsaAI 830 CACGTG [CT]AC^GT[GA]
839 CACGTG
BsaI 1945 GAGACC GGTCTC(1/5)
1871 GGTCTC
BseRI 790 GAGGAG GAGGAG(10/8)
1848 GAGGAG
BsiHKAI 793 GAGCAC G[AT]GC[AT]^C
1851 GAGCTC
BstXI 653 CCAGCAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
827 CCACACGTGTGG
BstYI 239 AGATCC [GA]^GATC[CT]
1757 AGATCT
DsaI 1490 CCACGG C^C[GA][CT]GG
1738 CCATGG
EaeI 1374 TGGCCA [CT]^GGCC[GA]
1451 TGGCCG
EarI 27 GAAGAG CTCTTC(1/4)
1171 GAAGAG
Ksp632I 27 GAAGAG CTCTTC(1/4)
1171 GAAGAG
MflI 239 AGATCC [GA]^GATC[CT]
1757 AGATCT
MslI 828 CACACGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2043 CACACTGGTG
PmaCI 830 CACGTG CAC^GTG
839 CACGTG
PmlI 830 CACGTG CAC^GTG
839 CACGTG
PstI 1109 CTGCAG CTGCA^G
1441 CTGCAG
SfaNI 1528 GATGC GCATC(5/9)
1927 GCATC
XhoII 239 AGATCC [GA]^GATC[CT]
1757 AGATCT
XmnI 624 GAACACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
958 GAAAATATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 65 enzymes) #
#########################################
AciI AfaI AflIII AluI AvaII
BbvI BcnI BfaI BmyI BpmI
BsaJI BsiYI BslI BsmAI BsmFI
Bsp1286I BspMI BsrI BssKI BstF5I
BstNI Cac8I Cfr13I DdeI DpnII
EcoRII Fnu4HI FokI HaeIII HapII
HincII HindII HinfI HpaII HphI
ItaI MaeI MaeII MaeIII MboI
MboII MnlI MseI MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
NspI RsaI Sau3AI Sau96I ScrFI
SfcI SmlI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I