Restriction map for
pF1KSDA0770
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# NO CUT ENZYMES ( 112 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AhdI
Alw44I Aor51HI ApaI ApaLI AscI
AseI AsnI Asp718I AspEI AspI
AviII AvrII BamHI BanI BbeI
BbsI BcgI BfrI BlnI BlpI
Bpu1102I BpuAI BsaHI BseAI BsiEI
BsiWI BsmBI Bsp1407I BspDI BspEI
BspLU11I BsrGI BssHII Bst1107I BstBI
BstEII BstPI BstUI BstZ17I CelII
ClaI CpoI DraI DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
EcoO65I EcoRV EcoT22I FauI FseI
FspI HaeII Hin1I HincII HindII
HindIII HpaI KasI KpnI KspI
MluI MroI MvnI NarI NdeI
NheI NotI NruI NsiI NspI
NspV PacI PflFI PmeI PshAI
PshBI Psp1406I PvuI RsrII SacII
SalI SexAI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI SspBI
SspI StuI SwaI Tth111I XbaI
XmaCI XmaI
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# ONE CUT ENZYMES ( 34 enzymes) #
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AgeI 352 ACCGGT A^CCGGT
AvaI 2290 CTCGAG C^[CT]CG[GA]G
BbvCI 2122 GCTGAGG CCTCAGC(-5/-2)
BclI 1269 TGATCA T^GATCA
BglI 1387 GCCCTGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1692 AGATCT A^GATCT
BsaBI 2173 GATGTGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 349 GAGACC GGTCTC(1/5)
BsaWI 352 ACCGGT [AT]^CCGG[AT]
BsmI 2216 GCATTC GAATGC(1/-1)
BsoBI 2290 CTCGAG C^[CT]CG[GA]G
BspHI 429 TCATGA T^CATGA
CfoI 2058 GCGC GCG^C
EcoRI 2449 GAATTC G^AATTC
FbaI 1269 TGATCA T^GATCA
HgaI 7 GACGC GACGC(5/10)
HhaI 2058 GCGC GCG^C
HinP1I 2058 GCGC G^CGC
MamI 2173 GATGTGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1243 CAATTG C^AATTG
MunI 1243 CAATTG C^AATTG
NaeI 1997 GCCGGC GCC^GGC
NgoMIV 1997 GCCGGC G^CCGGC
PaeR7I 2290 CTCGAG C^TCGAG
PinAI 352 ACCGGT A^CCGGT
PpuMI 2285 AGGTCCT [GA]G^G[AT]CC[CT]
RcaI 429 TCATGA T^CATGA
SapI 2467 GAAGAGC GCTCTTC(1/4)
SbfI 1891 CCTGCAGG CCTGCA^GG
ScaI 2129 AGTACT AGT^ACT
SfiI 1386 GGCCCTGGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
Sse8387I 1891 CCTGCAGG CCTGCA^GG
XcmI 1094 CCACCCATGTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 2290 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 26 enzymes) #
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AlwNI 1588 CAGTATCTG CAG[ACGT][ACGT][ACGT]^CTG
1597 CAGCATCTG
AocI 830 CCTCAGG CC^T[ACGT]AGG
2454 CCTGAGG
Asp700I 76 GAATGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2409 GAAACGGTTC
BalI 710 TGGCCA TGG^CCA
857 TGGCCA
BbrPI 1447 CACGTG CAC^GTG
1718 CACGTG
BciVI 2109 GGATAC GTATCC(6/5)
1155 GTATCC
BcnI 1181 CCGGG CC^[GC]GG
1698 CCCGG
BfaI 31 CTAG C^TAG
163 CTAG
BsaAI 1447 CACGTG [CT]AC^GT[GA]
1718 CACGTG
BsgI 669 CTGCAC GTGCAG(16/14)
1585 GTGCAG
BsrDI 738 CATTGC GCAATG(2/0)
1166 CATTGC
BstAPI 1587 GCAGTATCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2216 GCATTCACTGC
BstXI 467 CCAAGTCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2570 CCAATGGAGTGG
Bsu36I 830 CCTCAGG CC^T[ACGT]AGG
2454 CCTGAGG
EaeI 710 TGGCCA [CT]^GGCC[GA]
857 TGGCCA
Eco81I 830 CCTCAGG CC^T[ACGT]AGG
2454 CCTGAGG
EcoNI 1561 CCTCTGAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2376 CCTGGAAAAGG
MaeI 31 CTAG C^TAG
163 CTAG
MluNI 710 TGGCCA TGG^CCA
857 TGGCCA
MscI 710 TGGCCA TGG^CCA
857 TGGCCA
NciI 1181 CCGGG CC^[GC]GG
1698 CCCGG
PmaCI 1447 CACGTG CAC^GTG
1718 CACGTG
PmlI 1447 CACGTG CAC^GTG
1718 CACGTG
SacI 559 GAGCTC GAGCT^C
2042 GAGCTC
Tsp45I 157 GTGAC ^GT[GC]AC
332 GTGAC
XmnI 76 GAATGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2409 GAAACGGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 86 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AspHI AvaII BanII BbvI
BmyI BpmI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BspMI BsrBI BsrFI BsrI BssKI
BssSI BstF5I BstNI BstYI Cac8I
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EarI EcoO109I EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HinfI
HpaII HphI ItaI Ksp632I MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MwoI NcoI NdeII NlaIII NlaIV
PflMI PleI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp509I TspRI TthHB8I Van91I
XhoII