Restriction map for
pF1KSDA0775
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# NO CUT ENZYMES ( 114 enzymes) #
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AatII AccIII AclI AflII AgeI
AhdI Alw44I AocI Aor51HI ApaLI
AseI AsnI Asp700I AspEI AspI
AvaII AviII AvrII BalI BamHI
BbsI BcgI BciVI BfrI BlnI
BpuAI BsaBI BsaWI BseAI BsiEI
BsiWI BsmI Bsp1407I BspDI BspEI
BspHI BsrDI BsrGI BssSI Bst1107I
BstBI BstEII BstPI BstXI BstZ17I
Bsu36I ClaI CpoI DraI DrdI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoO65I EcoRI EcoRV EcoT14I
EcoT22I FseI FspI HincII HindII
HindIII HpaI MamI MfeI MluI
MluNI MroI MscI MseI MslI
MunI NcoI NdeI NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmeI PpuMI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SalI SapI SbfI ScaI SexAI
SfuI SnaBI SpeI SphI Sse8387I
SspBI SspI StyI SwaI Tru9I
Tth111I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 35 enzymes) #
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Acc65I 1787 GGTACC G^GTACC
AccI 211 GTCTAC GT^[AC][GT]AC
AcsI 876 GAATTT [GA]^AATT[CT]
AflIII 104 ACATGT A^C[GA][CT]GT
ApoI 876 GAATTT [GA]^AATT[CT]
AscI 1517 GGCGCGCC GG^CGCGCC
Asp718I 1787 GGTACC G^GTACC
BbeI 1417 GGCGCC GGCGC^C
BbrPI 1351 CACGTG CAC^GTG
BglII 482 AGATCT A^GATCT
BlpI 727 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 727 GCTCAGC GC^T[ACGT]AGC
BsaAI 1351 CACGTG [CT]AC^GT[GA]
BspLU11I 104 ACATGT A^CATGT
BstAPI 498 GCAGCAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII 727 GCTCAGC GC^T[ACGT]AGC
DsaI 1513 CCGCGG C^C[GA][CT]GG
KasI 1417 GGCGCC G^GCGCC
KpnI 1787 GGTACC GGTAC^C
KspI 1513 CCGCGG CCGC^GG
MaeII 1352 ACGT A^CGT
NarI 1417 GGCGCC GG^CGCC
NspI 104 ACATGT [GA]CATG^[CT]
PflMI 807 CCAGGATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 1351 CACGTG CAC^GTG
PmlI 1351 CACGTG CAC^GTG
SacI 1483 GAGCTC GAGCT^C
SacII 1513 CCGCGG CCGC^GG
SfiI 1440 GGCCGAAGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 1417 GGCGCC GGC^GCC
SgrAI 1199 CGCCGGTG C[GA]^CCGG[CT]G
TfiI 950 GAATC G^A[AT]TC
Tsp509I 877 AATT ^AATT
Van91I 807 CCAGGATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1598 CCAGCCCGGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 25 enzymes) #
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AcyI 197 GACGCC G[GA]^CG[CT]C
1417 GGCGCC
AfaI 225 GTAC GT^AC
1788 GTAC
AlwNI 499 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
808 CAGGATCTG
ApaI 455 GGGCCC GGGCC^C
803 GGGCCC
BbvCI 1506 CCTCAGC CCTCAGC(-5/-2)
441 GCTGAGG
BclI 233 TGATCA T^GATCA
867 TGATCA
BfaI 56 CTAG C^TAG
685 CTAG
BsaHI 197 GACGCC G[GA]^CG[CT]C
1417 GGCGCC
BsaI 83 GAGACC GGTCTC(1/5)
796 GAGACC
BsgI 576 CTGCAC GTGCAG(16/14)
592 CTGCAC
BsmBI 466 GAGACG CGTCTC(1/5)
1729 GAGACG
BspMI 473 ACCTGC ACCTGC(4/8)
590 ACCTGC
BssHII 1323 GCGCGC G^CGCGC
1518 GCGCGC
DraIII 653 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
1348 CACCACGTG
EarI 420 GAAGAG CTCTTC(1/4)
688 GAAGAG
EcoNI 33 CCTGACGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
291 CCTGCTGGAGG
FbaI 233 TGATCA T^GATCA
867 TGATCA
Hin1I 197 GACGCC G[GA]^CG[CT]C
1417 GGCGCC
Ksp632I 420 GAAGAG CTCTTC(1/4)
688 GAAGAG
MaeI 56 CTAG C^TAG
685 CTAG
NaeI 917 GCCGGC GCC^GGC
1360 GCCGGC
NgoMIV 917 GCCGGC G^CCGGC
1360 GCCGGC
RsaI 225 GTAC GT^AC
1788 GTAC
SmlI 624 CTTGAG C^T[CT][GA]AG
954 CTTGAG
StuI 395 AGGCCT AGG^CCT
519 AGGCCT
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# 3 OR MORE CUT ENZYMES ( 84 enzymes) #
#########################################
AccII AciI AluI AlwI AspHI
AvaI BanI BanII BbvI BcnI
BglI BmyI BpmI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BsrBI BsrFI BsrI
BssKI BstF5I BstNI BstUI BstYI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII EaeI EcoO109I EcoRII
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI HinP1I HinfI
HpaII HphI ItaI MaeIII MboI
MboII MflI MnlI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV PleI PstI PvuII
Sau3AI Sau96I ScrFI SfaNI SfcI
SmaI TaqI TseI Tsp45I TspRI
TthHB8I XhoII XmaCI XmaI