Restriction map for
pF1KSDA0776
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# NO CUT ENZYMES ( 132 enzymes) #
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AatII Acc65I AccII AccIII AclI
AflII AgeI AhdI Alw44I Aor51HI
ApaI ApaLI AscI Asp718I AspEI
AspHI AspI AviII AvrII BalI
BamHI BanI BanII BbeI BbrPI
BbvCI BcgI BclI BfrI BglI
BlnI BlpI BmyI Bpu1102I BsaBI
BsaI BsaWI BseAI BsiHKAI BsiWI
BsmBI Bsp1286I BspDI BspEI BspHI
BsrFI BssHII BssSI Bst1107I BstAPI
BstBI BstUI BstZ17I CelII Cfr10I
ClaI CpoI DraI DraII DraIII
DrdI Eam1105I Eco47III EcoO109I EcoT14I
EcoT22I FbaI FseI FspI HaeII
HincII HindII HpaI KasI KpnI
KspI MamI MfeI MluI MluNI
MroI MscI MslI MunI MvnI
NaeI NarI NcoI NdeI NgoMIV
NheI NlaIV NotI NruI NsiI
NspV PacI PflFI PflMI PinAI
PleI PmaCI PmeI PmlI PpuMI
PshAI Psp1406I PvuI RcaI RsrII
SacI SacII SalI SapI SbfI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI Sse8387I SspI
StyI SwaI Tth111I Van91I XcmI
XmaCI XmaI
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# ONE CUT ENZYMES ( 56 enzymes) #
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AccI 1789 GTAGAC GT^[AC][GT]AC
AcyI 7 GACGCC G[GA]^CG[CT]C
AflIII 210 ACATGT A^C[GA][CT]GT
AocI 1480 CCTGAGG CC^T[ACGT]AGG
AseI 256 ATTAAT AT^TAAT
AsnI 256 ATTAAT AT^TAAT
Asp700I 136 GAAGTAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 1540 CTCGAG C^[CT]CG[GA]G
AvaII 264 GGACC G^G[AT]CC
BciVI 566 GTATCC GTATCC(6/5)
BcnI 593 CCCGG CC^[GC]GG
BglII 1197 AGATCT A^GATCT
BsaAI 563 CACGTA [CT]AC^GT[GA]
BsaHI 7 GACGCC G[GA]^CG[CT]C
BsgI 2056 CTGCAC GTGCAG(16/14)
BsiEI 35 CGGCCG CG[GA][CT]^CG
BsmI 1042 GCATTC GAATGC(1/-1)
BsoBI 1540 CTCGAG C^[CT]CG[GA]G
BspLU11I 210 ACATGT A^CATGT
BspMI 1029 GCAGGT ACCTGC(4/8)
BsrBI 85 GAGCGG CCGCTC(-3/-3)
BstEII 408 GGTCACC G^GT[ACGT]ACC
BstPI 408 GGTCACC G^GT[ACGT]ACC
BstXI 2106 CCATGCACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 1197 AGATCT [GA]^GATC[CT]
Bsu36I 1480 CCTGAGG CC^T[ACGT]AGG
CfoI 52 GCGC GCG^C
Cfr13I 264 GGACC G^G[ACGT]CC
DsaI 570 CCGTGG C^C[GA][CT]GG
EaeI 35 CGGCCG [CT]^GGCC[GA]
EagI 35 CGGCCG C^GGCCG
EclXI 35 CGGCCG C^GGCCG
Eco52I 35 CGGCCG C^GGCCG
Eco81I 1480 CCTGAGG CC^T[ACGT]AGG
EcoNI 776 CCTTTTTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 408 GGTCACC G^GT[ACGT]ACC
EcoRI 609 GAATTC G^AATTC
EcoRV 1738 GATATC GAT^ATC
FauI 48 GCGGG CCCGC(4/6)
HapII 594 CCGG C^CGG
HhaI 52 GCGC GCG^C
Hin1I 7 GACGCC G[GA]^CG[CT]C
HinP1I 52 GCGC G^CGC
HpaII 594 CCGG C^CGG
MflI 1197 AGATCT [GA]^GATC[CT]
MspI 594 CCGG C^CGG
NciI 593 CCCGG CC^[GC]GG
PaeR7I 1540 CTCGAG C^TCGAG
PshBI 256 ATTAAT AT^TAAT
PvuII 602 CAGCTG CAG^CTG
Sau96I 264 GGACC G^G[ACGT]CC
StuI 1055 AGGCCT AGG^CCT
XbaI 798 TCTAGA T^CTAGA
XhoI 1540 CTCGAG C^TCGAG
XhoII 1197 AGATCT [GA]^GATC[CT]
XmnI 136 GAAGTAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AlwNI 833 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG
1987 CAGATACTG
BpmI 173 CTCCAG CTGGAG(16/14)
747 CTCCAG
BsiYI 68 CCACGCAGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
776 CCTTTTTCAGG
BslI 68 CCACGCAGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
776 CCTTTTTCAGG
BsmAI 711 GAGAC GTCTC(1/5)
1967 GAGAC
Bsp1407I 1114 TGTACA T^GTACA
1640 TGTACA
BsrDI 2136 CATTGC GCAATG(2/0)
1313 GCAATG
BsrGI 1114 TGTACA T^GTACA
1640 TGTACA
HgaI 7 GACGC GACGC(5/10)
683 GACGC
HindIII 539 AAGCTT A^AGCTT
1940 AAGCTT
MaeII 564 ACGT A^CGT
1551 ACGT
MspA1I 46 CAGCGG C[AC]G^C[GT]G
602 CAGCTG
PstI 1799 CTGCAG CTGCA^G
1931 CTGCAG
SmlI 661 CTTGAG C^T[CT][GA]AG
1540 CTCGAG
SphI 2099 GCATGC GCATG^C
2172 GCATGC
SspBI 1114 TGTACA T^GTACA
1640 TGTACA
#########################################
# 3 OR MORE CUT ENZYMES ( 54 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI BbsI BbvI BfaI BpuAI
BsaJI BseRI BsmFI BsrI BssKI
BstF5I BstNI Cac8I DdeI DpnII
EarI EcoRII Fnu4HI FokI HaeIII
HinfI HphI ItaI Ksp632I MaeI
MaeIII MboI MboII MnlI MseI
MvaI MwoI NdeII NlaIII NspI
RsaI Sau3AI ScaI ScrFI SfaNI
SfcI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I