Restriction map for
pF1KSDA0778
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# NO CUT ENZYMES ( 82 enzymes) #
##################################
AccI AclI AflII AhdI Aor51HI
AscI AseI AsnI AspEI AviII
AvrII BamHI BbeI BfaI BfrI
BglII BlnI BlpI Bpu1102I BsiEI
BsiWI Bsp1407I BspDI BsrGI BssHII
Bst1107I BstBI BstZ17I CelII ClaI
CpoI DraI EagI Eam1105I EclXI
Eco47III Eco52I EcoRI EcoT22I FseI
FspI HaeII HpaI KasI KspI
MaeI MfeI MluI MunI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PmeI
PpuMI PshAI PshBI Psp1406I PstI
PvuI RsrII SacII SalI SapI
SbfI SfiI SfoI SfuI SgrAI
SnaBI SpeI Sse8387I SspBI SspI
SwaI XbaI
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# ONE CUT ENZYMES ( 53 enzymes) #
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AatII 2209 GACGTC GACGT^C
Acc65I 486 GGTACC G^GTACC
AccIII 602 TCCGGA T^CCGGA
AcsI 1699 AAATTC [GA]^AATT[CT]
AcyI 2209 GACGTC G[GA]^CG[CT]C
AflIII 1163 ACATGT A^C[GA][CT]GT
AgeI 1538 ACCGGT A^CCGGT
Alw44I 1984 GTGCAC G^TGCAC
ApaLI 1984 GTGCAC G^TGCAC
ApoI 1699 AAATTC [GA]^AATT[CT]
Asp700I 2616 GAACGGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 486 GGTACC G^GTACC
AspI 583 GACCGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbvCI 490 CCTCAGC CCTCAGC(-5/-2)
BciVI 3024 GTATCC GTATCC(6/5)
BsaBI 525 GATGCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 2209 GACGTC G[GA]^CG[CT]C
BseAI 602 TCCGGA T^CCGGA
BspEI 602 TCCGGA T^CCGGA
BspHI 2483 TCATGA T^CATGA
BspLU11I 1163 ACATGT A^CATGT
BspMI 30 ACCTGC ACCTGC(4/8)
BsrFI 1538 ACCGGT [GA]^CCGG[CT]
BssSI 1471 CACGAG CACGAG(-5/-1)
BstAPI 2752 GCATTCTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1836 GGTAACC G^GT[ACGT]ACC
BstPI 1836 GGTAACC G^GT[ACGT]ACC
Cfr10I 1538 ACCGGT [GA]^CCGG[CT]
DrdI 1248 GACGGCCCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 612 CTCTTC CTCTTC(1/4)
EcoO65I 1836 GGTAACC G^GT[ACGT]ACC
EcoRV 2479 GATATC GAT^ATC
Hin1I 2209 GACGTC G[GA]^CG[CT]C
HindIII 1735 AAGCTT A^AGCTT
KpnI 486 GGTACC GGTAC^C
Ksp632I 612 CTCTTC CTCTTC(1/4)
MamI 525 GATGCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 602 TCCGGA T^CCGGA
MseI 646 TTAA T^TAA
PaeR7I 212 CTCGAG C^TCGAG
PflFI 583 GACCGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 1538 ACCGGT A^CCGGT
RcaI 2483 TCATGA T^CATGA
ScaI 23 AGTACT AGT^ACT
SmaI 1775 CCCGGG CCC^GGG
SphI 1975 GCATGC GCATG^C
StuI 819 AGGCCT AGG^CCT
Tru9I 646 TTAA T^TAA
Tth111I 583 GACCGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 212 CTCGAG C^TCGAG
XmaCI 1775 CCCGGG C^CCGGG
XmaI 1775 CCCGGG C^CCGGG
XmnI 2616 GAACGGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AccII 586 CGCG CG^CG
1282 CGCG
AlwNI 1790 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG
2572 CAGGCACTG
AocI 815 CCTCAGG CC^T[ACGT]AGG
991 CCTGAGG
ApaI 220 GGGCCC GGGCC^C
1517 GGGCCC
BalI 980 TGGCCA TGG^CCA
1004 TGGCCA
BanI 486 GGTACC G^G[CT][GA]CC
1129 GGCACC
BbrPI 1498 CACGTG CAC^GTG
2739 CACGTG
BcgI 2151 CGACTCCCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1148 GCATGACCGTCG
BclI 875 TGATCA T^GATCA
1829 TGATCA
BsgI 1558 GTGCAG GTGCAG(16/14)
2776 GTGCAG
BsmI 1529 GCATTC GAATGC(1/-1)
2752 GCATTC
BstUI 586 CGCG CG^CG
1282 CGCG
Bsu36I 815 CCTCAGG CC^T[ACGT]AGG
991 CCTGAGG
Eco81I 815 CCTCAGG CC^T[ACGT]AGG
991 CCTGAGG
FbaI 875 TGATCA T^GATCA
1829 TGATCA
HgaI 1089 GACGC GACGC(5/10)
587 GCGTC
HincII 1658 GTCAAC GT[CT]^[GA]AC
1954 GTCAAC
HindII 1658 GTCAAC GT[CT]^[GA]AC
1954 GTCAAC
MluNI 980 TGGCCA TGG^CCA
1004 TGGCCA
MscI 980 TGGCCA TGG^CCA
1004 TGGCCA
MvnI 586 CGCG CG^CG
1282 CGCG
NcoI 353 CCATGG C^CATGG
2189 CCATGG
PflMI 1497 CCACGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2571 CCAGGCACTGG
PmaCI 1498 CACGTG CAC^GTG
2739 CACGTG
PmlI 1498 CACGTG CAC^GTG
2739 CACGTG
SacI 453 GAGCTC GAGCT^C
1375 GAGCTC
SexAI 560 ACCTGGT A^CC[AT]GGT
2947 ACCTGGT
SfcI 936 CTACAG C^T[GA][CT]AG
2970 CTACAG
Tsp509I 1263 AATT ^AATT
1700 AATT
Van91I 1497 CCACGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2571 CCAGGCACTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 93 enzymes) #
#########################################
AciI AfaI AluI AlwI AspHI
AvaI AvaII BanII BbsI BbvI
BcnI BglI BmyI BpmI BpuAI
BsaAI BsaI BsaJI BsaWI BseRI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI BsoBI Bsp1286I BsrBI BsrDI
BsrI BssKI BstF5I BstNI BstXI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII DraIII DsaI EaeI
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
MaeII MaeIII MboI MboII MflI
MnlI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
NspI PleI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SmlI StyI
TaqI TfiI TseI Tsp45I TspRI
TthHB8I XcmI XhoII