Restriction map for
pF1KSDA0780
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# NO CUT ENZYMES ( 86 enzymes) #
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AatII Acc65I AccII AclI AgeI
AlwNI Aor51HI AscI Asp718I AviII
AvrII BbrPI BcgI BciVI BglI
BlnI BsaAI BsaBI BsmBI Bsp1407I
BspDI BsrBI BsrFI BsrGI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstUI BstZ17I Cfr10I ClaI CpoI
DraI EagI EclXI Eco47III Eco52I
EcoO65I FseI FspI HgaI KpnI
KspI MamI MfeI MluI MunI
MvnI NaeI NgoMIV NotI NruI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI PshAI Psp1406I PvuI
RsrII SacI SacII SalI SapI
SbfI SfiI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SwaI XbaI XcmI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 35 enzymes) #
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AccIII 642 TCCGGA T^CCGGA
AflIII 2891 ACATGT A^C[GA][CT]GT
AhdI 2588 GACATAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 921 GTGCAC G^TGCAC
ApaLI 921 GTGCAC G^TGCAC
Asp700I 288 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 2588 GACATAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 1489 CCCGAG C^[CT]CG[GA]G
BamHI 2917 GGATCC G^GATCC
BlpI 1888 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1888 GCTGAGC GC^T[ACGT]AGC
BsaI 2457 GGTCTC GGTCTC(1/5)
BsaWI 642 TCCGGA [AT]^CCGG[AT]
BseAI 642 TCCGGA T^CCGGA
BsiEI 2487 CGGTCG CG[GA][CT]^CG
BsiWI 2166 CGTACG C^GTACG
BsmI 2260 GAATGC GAATGC(1/-1)
BsoBI 1489 CCCGAG C^[CT]CG[GA]G
BspEI 642 TCCGGA T^CCGGA
BspLU11I 2891 ACATGT A^CATGT
BstAPI 2429 GCATCTTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII 1888 GCTGAGC GC^T[ACGT]AGC
DraIII 1928 CACACTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1225 GACCTCAAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 2588 GACATAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FauI 130 GCGGG CCCGC(4/6)
HpaI 1569 GTTAAC GTT^AAC
MroI 642 TCCGGA T^CCGGA
NdeI 821 CATATG CA^TATG
NheI 1303 GCTAGC G^CTAGC
PflMI 620 CCAAGTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
ScaI 365 AGTACT AGT^ACT
SexAI 2897 ACCAGGT A^CC[AT]GGT
Van91I 620 CCAAGTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 288 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 37 enzymes) #
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AccI 1131 GTCTAC GT^[AC][GT]AC
1470 GTCTAC
AcyI 1712 GGCGCC G[GA]^CG[CT]C
1770 GGCGCC
AflII 1365 CTTAAG C^TTAAG
2293 CTTAAG
ApaI 2318 GGGCCC GGGCC^C
2802 GGGCCC
AspHI 921 GTGCAC G[AT]GC[AT]^C
2288 GTGCTC
AspI 1210 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
1866 GACGAAGTC
BbeI 1712 GGCGCC GGCGC^C
1770 GGCGCC
BbvCI 1263 GCTGAGG CCTCAGC(-5/-2)
2812 GCTGAGG
BclI 809 TGATCA T^GATCA
1029 TGATCA
BfrI 1365 CTTAAG C^TTAAG
2293 CTTAAG
BglII 1741 AGATCT A^GATCT
2207 AGATCT
BsaHI 1712 GGCGCC G[GA]^CG[CT]C
1770 GGCGCC
BsiHKAI 921 GTGCAC G[AT]GC[AT]^C
2288 GTGCTC
BspHI 806 TCATGA T^CATGA
2202 TCATGA
BsrDI 1979 GCAATG GCAATG(2/0)
2929 GCAATG
DraII 1196 AGGTCCC [GA]G^G[ACGT]CC[CT]
3019 GGGACCT
EcoNI 1136 CCTCTAAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2118 CCTTGAAGAGG
EcoO109I 1196 AGGTCCC [GA]G^G[ACGT]CC[CT]
3019 GGGACCT
EcoRI 802 GAATTC G^AATTC
3033 GAATTC
FbaI 809 TGATCA T^GATCA
1029 TGATCA
HaeII 1712 GGCGCC [GA]GCGC^[CT]
1770 GGCGCC
Hin1I 1712 GGCGCC G[GA]^CG[CT]C
1770 GGCGCC
HincII 1569 GTTAAC GT[CT]^[GA]AC
2596 GTCAAC
HindII 1569 GTTAAC GT[CT]^[GA]AC
2596 GTCAAC
HindIII 1113 AAGCTT A^AGCTT
1409 AAGCTT
KasI 1712 GGCGCC G^GCGCC
1770 GGCGCC
MaeII 556 ACGT A^CGT
2606 ACGT
MslI 1603 CATGGGAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2634 CATTTCCGTG
NarI 1712 GGCGCC GG^CGCC
1770 GGCGCC
NcoI 65 CCATGG C^CATGG
1602 CCATGG
PflFI 1210 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
1866 GACGAAGTC
PpuMI 1196 AGGTCCC [GA]G^G[AT]CC[CT]
3019 GGGACCT
PstI 1078 CTGCAG CTGCA^G
2436 CTGCAG
RcaI 806 TCATGA T^CATGA
2202 TCATGA
SfoI 1712 GGCGCC GGC^GCC
1770 GGCGCC
StuI 1144 AGGCCT AGG^CCT
1729 AGGCCT
Tth111I 1210 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
1866 GACGAAGTC
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# 3 OR MORE CUT ENZYMES ( 100 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AocI ApoI AseI AsnI AvaII
BalI BanI BanII BbsI BbvI
BcnI BfaI BmyI BpmI BpuAI
BsaJI BseRI BsgI BsiYI BslI
BsmAI BsmFI Bsp1286I BspMI BsrI
BssKI BstF5I BstNI BstXI BstYI
Bsu36I Cac8I CfoI Cfr13I DdeI
DpnII DsaI EaeI EarI Eco81I
EcoRII EcoRV EcoT14I EcoT22I Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV NsiI NspI PleI
PshBI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SspI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII