Restriction map for
pF1KSDA0782
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# NO CUT ENZYMES ( 64 enzymes) #
##################################
AatII AclI AflII AgeI AscI
AseI AsnI AvrII BamHI BbeI
BcgI BfrI BglII BlnI BsiWI
BspDI BspLU11I BsrDI BssSI Bst1107I
BstBI BstEII BstPI BstZ17I ClaI
CpoI DraI DrdI EagI EclXI
Eco52I EcoO65I EcoT22I FseI HindIII
HpaI KasI NarI NdeI NheI
NotI NruI NsiI NspV PacI
PaeR7I PinAI PmeI PshAI PshBI
Psp1406I RsrII SalI SbfI ScaI
SfoI SfuI SgrAI SnaBI SpeI
Sse8387I SspI SwaI XhoI
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# ONE CUT ENZYMES ( 33 enzymes) #
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AccIII 3215 TCCGGA T^CCGGA
AcsI 1405 GAATTC [GA]^AATT[CT]
AflIII 3483 ACGCGT A^C[GA][CT]GT
ApoI 1405 GAATTC [GA]^AATT[CT]
AspI 1597 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 3605 TGCGCA TGC^GCA
BciVI 321 GGATAC GTATCC(6/5)
BsaBI 322 GATACTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 3215 TCCGGA T^CCGGA
BsmBI 1014 CGTCTC CGTCTC(1/5)
BsmI 1807 GCATTC GAATGC(1/-1)
BspEI 3215 TCCGGA T^CCGGA
BspHI 1779 TCATGA T^CATGA
BssHII 2299 GCGCGC G^CGCGC
EcoRI 1405 GAATTC G^AATTC
EcoRV 2170 GATATC GAT^ATC
FspI 3605 TGCGCA TGC^GCA
KspI 3584 CCGCGG CCGC^GG
MamI 322 GATACTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 2585 CAATTG C^AATTG
MluI 3483 ACGCGT A^CGCGT
MroI 3215 TCCGGA T^CCGGA
MunI 2585 CAATTG C^AATTG
NspI 2772 GCATGC [GA]CATG^[CT]
PflFI 1597 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PvuI 3165 CGATCG CGAT^CG
RcaI 1779 TCATGA T^CATGA
SacII 3584 CCGCGG CCGC^GG
SfiI 3045 GGCCATGGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SphI 2772 GCATGC GCATG^C
StuI 2423 AGGCCT AGG^CCT
Tth111I 1597 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 675 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 39 enzymes) #
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Acc65I 2462 GGTACC G^GTACC
3386 GGTACC
AccI 861 GTCTAC GT^[AC][GT]AC
3295 GTCTAC
AhdI 2137 GACACGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3589 GACCCTCTGTC
Alw44I 2308 GTGCAC G^TGCAC
2572 GTGCAC
ApaLI 2308 GTGCAC G^TGCAC
2572 GTGCAC
Asp700I 1104 GAACCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1405 GAATTCCTTC
Asp718I 2462 GGTACC G^GTACC
3386 GGTACC
AspEI 2137 GACACGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3589 GACCCTCTGTC
BalI 53 TGGCCA TGG^CCA
3071 TGGCCA
BbrPI 400 CACGTG CAC^GTG
2332 CACGTG
BclI 327 TGATCA T^GATCA
434 TGATCA
BlpI 63 GCTGAGC GC^T[ACGT]AGC
520 GCTGAGC
Bpu1102I 63 GCTGAGC GC^T[ACGT]AGC
520 GCTGAGC
BsaWI 1421 ACCGGA [AT]^CCGG[AT]
3215 TCCGGA
BsiEI 1490 CGACCG CG[GA][CT]^CG
3165 CGATCG
Bsp1407I 1724 TGTACA T^GTACA
2060 TGTACA
BsrGI 1724 TGTACA T^GTACA
2060 TGTACA
BstAPI 498 GCAGGCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1389 GCAGACGCTGC
CelII 63 GCTGAGC GC^T[ACGT]AGC
520 GCTGAGC
Eam1105I 2137 GACACGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3589 GACCCTCTGTC
FbaI 327 TGATCA T^GATCA
434 TGATCA
HincII 2494 GTCAAC GT[CT]^[GA]AC
2938 GTCAAC
HindII 2494 GTCAAC GT[CT]^[GA]AC
2938 GTCAAC
KpnI 2462 GGTACC GGTAC^C
3386 GGTACC
MluNI 53 TGGCCA TGG^CCA
3071 TGGCCA
MscI 53 TGGCCA TGG^CCA
3071 TGGCCA
MseI 2660 TTAA T^TAA
3281 TTAA
NaeI 1531 GCCGGC GCC^GGC
1574 GCCGGC
NgoMIV 1531 GCCGGC G^CCGGC
1574 GCCGGC
PmaCI 400 CACGTG CAC^GTG
2332 CACGTG
PmlI 400 CACGTG CAC^GTG
2332 CACGTG
PvuII 1084 CAGCTG CAG^CTG
3189 CAGCTG
SexAI 2024 ACCAGGT A^CC[AT]GGT
3023 ACCTGGT
SmlI 2314 CTCAAG C^T[CT][GA]AG
2356 CTCAAG
SspBI 1724 TGTACA T^GTACA
2060 TGTACA
TfiI 672 GAATC G^A[AT]TC
1650 GATTC
Tru9I 2660 TTAA T^TAA
3281 TTAA
Tsp509I 1406 AATT ^AATT
2586 AATT
XmnI 1104 GAACCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1405 GAATTCCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 122 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AocI Aor51HI ApaI
AspHI AvaI AvaII BanI BanII
BbsI BbvCI BbvI BcnI BfaI
BglI BmyI BpmI BpuAI BsaAI
BsaHI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrBI BsrFI
BsrI BssKI BstF5I BstNI BstUI
BstXI BstYI Bsu36I Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DraIII DsaI EaeI EarI Eco47III
Eco81I EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MslI MspA1I MspI
MvaI MvnI MwoI NciI NcoI
NdeII NlaIII NlaIV PflMI PleI
PpuMI PstI RsaI SacI SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmaI StyI TaqI TseI Tsp45I
TspRI TthHB8I Van91I XcmI XhoII
XmaCI XmaI