Restriction map for
pF1KSDA0792
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# NO CUT ENZYMES ( 96 enzymes) #
##################################
Acc65I AccIII AclI AcsI AflII
AgeI Alw44I Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp718I
AviII AvrII BbeI BcgI BfrI
BglII BlnI BlpI Bpu1102I BseAI
BsiEI BsiWI BsmBI BsmI Bsp1407I
BspDI BspEI BspLU11I BsrBI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstZ17I CelII ClaI CpoI DraI
EagI EclXI Eco47III Eco52I EcoRI
EcoRV FseI FspI HindIII HpaI
KasI KpnI KspI MfeI MroI
MunI NaeI NarI NdeI NgoMIV
NheI NotI NruI NspV PacI
PaeR7I PinAI PmeI PpuMI PshBI
Psp1406I PvuI RsrII SacII SalI
ScaI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI SspBI
SspI SwaI Tsp509I XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AatII 169 GACGTC GACGT^C
AhdI 262 GACAGCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp700I 1092 GAAAGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 262 GACAGCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1479 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 67 CTCGGG C^[CT]CG[GA]G
BamHI 1985 GGATCC G^GATCC
BbrPI 759 CACGTG CAC^GTG
BbvCI 2166 CCTCAGC CCTCAGC(-5/-2)
BciVI 753 GTATCC GTATCC(6/5)
BclI 1754 TGATCA T^GATCA
BcnI 744 CCGGG CC^[GC]GG
BfaI 1573 CTAG C^TAG
BglI 1762 GCCAAGACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaBI 284 GATTGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 1586 TCCGGT [AT]^CCGG[AT]
BsoBI 67 CTCGGG C^[CT]CG[GA]G
BspHI 1502 TCATGA T^CATGA
BstEII 132 GGTCACC G^GT[ACGT]ACC
BstPI 132 GGTCACC G^GT[ACGT]ACC
BstXI 1622 CCATCAGGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraIII 1927 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 2292 GACAGCACTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 262 GACAGCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 132 GGTCACC G^GT[ACGT]ACC
EcoT22I 1832 ATGCAT ATGCA^T
FbaI 1754 TGATCA T^GATCA
HaeII 2383 AGCGCC [GA]GCGC^[CT]
MaeI 1573 CTAG C^TAG
MamI 284 GATTGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI 749 ACGCGT A^CGCGT
NciI 744 CCGGG CC^[GC]GG
NsiI 1832 ATGCAT ATGCA^T
NspI 2231 ACATGC [GA]CATG^[CT]
PflFI 1479 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 759 CACGTG CAC^GTG
PmlI 759 CACGTG CAC^GTG
PshAI 1509 GACCAAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 865 CTGCAG CTGCA^G
RcaI 1502 TCATGA T^CATGA
SacI 1165 GAGCTC GAGCT^C
SbfI 864 CCTGCAGG CCTGCA^GG
SexAI 671 ACCTGGT A^CC[AT]GGT
SmlI 1921 CTCAAG C^T[CT][GA]AG
Sse8387I 864 CCTGCAGG CCTGCA^GG
TaqI 292 TCGA T^CGA
Tth111I 1479 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 292 TCGA T^CGA
XbaI 1572 TCTAGA T^CTAGA
XmnI 1092 GAAAGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AccII 750 CGCG CG^CG
1893 CGCG
AfaI 657 GTAC GT^AC
1812 GTAC
AflIII 749 ACGCGT A^C[GA][CT]GT
760 ACGTGT
AocI 302 CCTCAGG CC^T[ACGT]AGG
1207 CCTGAGG
AspHI 121 GTGCTC G[AT]GC[AT]^C
1165 GAGCTC
BanII 1138 GAGCCC G[GA]GC[CT]^C
1165 GAGCTC
BsaAI 759 CACGTG [CT]AC^GT[GA]
2254 TACGTG
BsaI 1561 GGTCTC GGTCTC(1/5)
1729 GGTCTC
BseRI 300 CTCCTC GAGGAG(10/8)
664 GAGGAG
BsgI 583 CTGCAC GTGCAG(16/14)
778 GTGCAG
BsiHKAI 121 GTGCTC G[AT]GC[AT]^C
1165 GAGCTC
BsrDI 240 CATTGC GCAATG(2/0)
1700 GCAATG
BsrFI 74 ACCGGC [GA]^CCGG[CT]
1937 ACCGGC
BstUI 750 CGCG CG^CG
1893 CGCG
BstYI 380 AGATCC [GA]^GATC[CT]
1985 GGATCC
Bsu36I 302 CCTCAGG CC^T[ACGT]AGG
1207 CCTGAGG
Cfr10I 74 ACCGGC [GA]^CCGG[CT]
1937 ACCGGC
DraII 1241 AGGGCCT [GA]G^G[ACGT]CC[CT]
2078 AGGCCCC
Eco81I 302 CCTCAGG CC^T[ACGT]AGG
1207 CCTGAGG
EcoO109I 1241 AGGGCCT [GA]G^G[ACGT]CC[CT]
2078 AGGCCCC
FauI 2083 CCCGC CCCGC(4/6)
2351 GCGGG
HincII 308 GTCAAC GT[CT]^[GA]AC
490 GTCAAC
HindII 308 GTCAAC GT[CT]^[GA]AC
490 GTCAAC
MflI 380 AGATCC [GA]^GATC[CT]
1985 GGATCC
MslI 972 CACACGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1752 CATGATCATG
MvnI 750 CGCG CG^CG
1893 CGCG
NcoI 1073 CCATGG C^CATGG
1334 CCATGG
RsaI 657 GTAC GT^AC
1812 GTAC
SapI 840 GAAGAGC GCTCTTC(1/4)
2191 GAAGAGC
TfiI 221 GATTC G^A[AT]TC
2265 GATTC
XhoII 380 AGATCC [GA]^GATC[CT]
1985 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 81 enzymes) #
#########################################
AccI AciI AcyI AluI AlwI
AlwNI AvaII BalI BanI BbsI
BbvI BmyI BpmI BpuAI BsaHI
BsaJI BsiYI BslI BsmAI BsmFI
Bsp1286I BspMI BsrI BssKI BstF5I
BstNI Cac8I CfoI Cfr13I DdeI
DpnII DsaI EaeI EarI EcoNI
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeII MaeIII MboI MboII MluNI
MnlI MscI MseI MspA1I MspI
MvaI MwoI NdeII NlaIII NlaIV
PflMI PleI PvuII Sau3AI Sau96I
ScrFI SfaNI SfcI StuI StyI
Tru9I TseI Tsp45I TspRI Van91I
XcmI