Restriction map for
pF1KSDA0813
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# NO CUT ENZYMES ( 64 enzymes) #
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AatII Acc65I AclI AgeI AhdI
Aor51HI AscI AseI AsnI Asp718I
AspEI AvrII BclI BglII BlnI
BsiWI BspDI BspHI BssHII Bst1107I
BstBI BstEII BstPI BstZ17I ClaI
Eam1105I Eco47III EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI HpaI KpnI
KspI MfeI MluI MunI NaeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PinAI PmeI
PshBI Psp1406I PvuI RcaI SacII
SalI SfuI SgrAI SnaBI SpeI
SspI SwaI XbaI XhoI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AccI 2340 GTCTAC GT^[AC][GT]AC
AcsI 480 AAATTT [GA]^AATT[CT]
AflII 2394 CTTAAG C^TTAAG
AflIII 365 ACATGT A^C[GA][CT]GT
Alw44I 855 GTGCAC G^TGCAC
AocI 4301 CCTCAGG CC^T[ACGT]AGG
ApaLI 855 GTGCAC G^TGCAC
ApoI 480 AAATTT [GA]^AATT[CT]
BbrPI 2967 CACGTG CAC^GTG
BbsI 2643 GAAGAC GAAGAC(2/6)
BciVI 1954 GTATCC GTATCC(6/5)
BfaI 2044 CTAG C^TAG
BfrI 2394 CTTAAG C^TTAAG
BlpI 1460 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1460 GCTCAGC GC^T[ACGT]AGC
BpuAI 2643 GAAGAC GAAGAC(2/6)
BsaAI 2967 CACGTG [CT]AC^GT[GA]
BsaBI 1653 GATTTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 43 CGGCCG CG[GA][CT]^CG
BspLU11I 365 ACATGT A^CATGT
Bsu36I 4301 CCTCAGG CC^T[ACGT]AGG
CelII 1460 GCTCAGC GC^T[ACGT]AGC
CpoI 38 CGGTCCG CG^G[AT]CCG
DraI 1681 TTTAAA TTT^AAA
EagI 43 CGGCCG C^GGCCG
EclXI 43 CGGCCG C^GGCCG
Eco52I 43 CGGCCG C^GGCCG
Eco81I 4301 CCTCAGG CC^T[ACGT]AGG
HindIII 452 AAGCTT A^AGCTT
MaeI 2044 CTAG C^TAG
MamI 1653 GATTTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NdeI 607 CATATG CA^TATG
PmaCI 2967 CACGTG CAC^GTG
PmlI 2967 CACGTG CAC^GTG
PshAI 2333 GACAGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RsrII 38 CGGTCCG CG^G[AT]CCG
SacI 49 GAGCTC GAGCT^C
SapI 711 GCTCTTC GCTCTTC(1/4)
SbfI 2124 CCTGCAGG CCTGCA^GG
ScaI 1475 AGTACT AGT^ACT
SexAI 4403 ACCAGGT A^CC[AT]GGT
SfiI 35 GGCCGGTCCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 13 CCCGGG CCC^GGG
SphI 3212 GCATGC GCATG^C
Sse8387I 2124 CCTGCAGG CCTGCA^GG
XmaCI 13 CCCGGG C^CCGGG
XmaI 13 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 17 enzymes) #
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AccIII 1811 TCCGGA T^CCGGA
3144 TCCGGA
Asp700I 1314 GAACCCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2310 GAACCAGTTC
AspI 1528 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
4384 GACCCTGTC
BamHI 224 GGATCC G^GATCC
3602 GGATCC
BcgI 3627 CGACCACATTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
866 GCAATGGCATCG
BsaI 925 GAGACC GGTCTC(1/5)
3579 GGTCTC
BseAI 1811 TCCGGA T^CCGGA
3144 TCCGGA
BsmBI 1378 GAGACG CGTCTC(1/5)
3715 GAGACG
BspEI 1811 TCCGGA T^CCGGA
3144 TCCGGA
DrdI 2224 GACACCGTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2565 GACCTCCCTGTC
EarI 712 CTCTTC CTCTTC(1/4)
771 GAAGAG
Ksp632I 712 CTCTTC CTCTTC(1/4)
771 GAAGAG
MroI 1811 TCCGGA T^CCGGA
3144 TCCGGA
PflFI 1528 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
4384 GACCCTGTC
Tth111I 1528 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
4384 GACCCTGTC
XcmI 4086 CCACTGCGAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
4203 CCAGTGCCAGGATGG
XmnI 1314 GAACCCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2310 GAACCAGTTC
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# 3 OR MORE CUT ENZYMES ( 130 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI ApaI AspHI AvaI
AvaII AviII BalI BanI BanII
BbeI BbvCI BbvI BcnI BglI
BmyI BpmI BsaHI BsaJI BsaWI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI BsoBI Bsp1286I
Bsp1407I BspMI BsrBI BsrDI BsrFI
BsrGI BsrI BssKI BssSI BstAPI
BstF5I BstNI BstUI BstXI BstYI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DraIII DsaI EaeI
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI FspI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HincII HindII HinfI HpaII HphI
ItaI KasI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NarI NciI NcoI
NdeII NlaIII NlaIV NspI PflMI
PleI PpuMI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SfoI SmlI SspBI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XhoII