Restriction map for
pF1KSDA0828
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# NO CUT ENZYMES ( 104 enzymes) #
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Acc65I AccIII AclI AflII AgeI
AhdI Alw44I AlwNI Aor51HI ApaLI
AscI AseI AsnI Asp718I AspEI
AviII AvrII BamHI BbrPI BbsI
BbvCI BcgI BclI BfrI BlnI
BpuAI BsaAI BsaBI BsaWI BseAI
BsiWI BsmBI BsmI BspEI BspHI
BsrDI BsrFI BssHII BssSI Bst1107I
BstAPI BstBI BstZ17I Cfr10I CpoI
DraI DraIII Eam1105I Eco47III EcoNI
EcoRV EcoT22I FbaI FseI FspI
HindIII HpaI KpnI MamI MfeI
MluI MroI MslI MunI NaeI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PstI PvuI PvuII
RcaI RsrII SalI SapI SbfI
SexAI SfiI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspI StuI
SwaI Van91I XbaI XhoI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AatII 416 GACGTC GACGT^C
AccI 1681 GTCTAC GT^[AC][GT]AC
AflIII 968 ACATGT A^C[GA][CT]GT
Asp700I 1123 GAATCAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 1078 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 1715 TGGCCA TGG^CCA
BciVI 960 GTATCC GTATCC(6/5)
BglII 1498 AGATCT A^GATCT
BlpI 467 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 467 GCTCAGC GC^T[ACGT]AGC
BsaI 143 GAGACC GGTCTC(1/5)
BseRI 997 GAGGAG GAGGAG(10/8)
BsgI 7 GTGCAG GTGCAG(16/14)
BsiEI 104 CGGCCG CG[GA][CT]^CG
Bsp1407I 1357 TGTACA T^GTACA
BspDI 874 ATCGAT AT^CGAT
BspLU11I 968 ACATGT A^CATGT
BsrGI 1357 TGTACA T^GTACA
BstEII 1377 GGTAACC G^GT[ACGT]ACC
BstPI 1377 GGTAACC G^GT[ACGT]ACC
CelII 467 GCTCAGC GC^T[ACGT]AGC
ClaI 874 ATCGAT AT^CGAT
DrdI 325 GACGGCACCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 104 CGGCCG C^GGCCG
EclXI 104 CGGCCG C^GGCCG
Eco52I 104 CGGCCG C^GGCCG
EcoO65I 1377 GGTAACC G^GT[ACGT]ACC
EcoRI 388 GAATTC G^AATTC
HgaI 247 GCGTC GACGC(5/10)
HincII 1507 GTTGAC GT[CT]^[GA]AC
HindII 1507 GTTGAC GT[CT]^[GA]AC
MluNI 1715 TGGCCA TGG^CCA
MscI 1715 TGGCCA TGG^CCA
NspI 968 ACATGT [GA]CATG^[CT]
PflFI 1078 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 59 AGGACCT [GA]G^G[AT]CC[CT]
SacI 647 GAGCTC GAGCT^C
ScaI 1542 AGTACT AGT^ACT
SmaI 192 CCCGGG CCC^GGG
SspBI 1357 TGTACA T^GTACA
TfiI 1123 GAATC G^A[AT]TC
Tth111I 1078 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 192 CCCGGG C^CCGGG
XmaI 192 CCCGGG C^CCGGG
XmnI 1123 GAATCAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 26 enzymes) #
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AcsI 388 GAATTC [GA]^AATT[CT]
1096 AAATTT
AlwI 363 GATCC GGATC(4/5)
933 GGATC
ApoI 388 GAATTC [GA]^AATT[CT]
1096 AAATTT
AvaI 192 CCCGGG C^[CT]CG[GA]G
198 CTCGGG
BbeI 115 GGCGCC GGCGC^C
342 GGCGCC
BfaI 91 CTAG C^TAG
808 CTAG
BglI 124 GCCCCCCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
320 GCCCCGACGGC
BsoBI 192 CCCGGG C^[CT]CG[GA]G
198 CTCGGG
BspMI 1727 ACCTGC ACCTGC(4/8)
9 GCAGGT
BsrBI 212 CCGCTC CCGCTC(-3/-3)
172 GAGCGG
EarI 590 GAAGAG CTCTTC(1/4)
642 GAAGAG
HaeII 115 GGCGCC [GA]GCGC^[CT]
342 GGCGCC
KasI 115 GGCGCC G^GCGCC
342 GGCGCC
Ksp632I 590 GAAGAG CTCTTC(1/4)
642 GAAGAG
KspI 23 CCGCGG CCGC^GG
167 CCGCGG
MaeI 91 CTAG C^TAG
808 CTAG
NarI 115 GGCGCC GG^CGCC
342 GGCGCC
NcoI 119 CCATGG C^CATGG
1235 CCATGG
SacII 23 CCGCGG CCGC^GG
167 CCGCGG
SfaNI 1741 GATGC GCATC(5/9)
1415 GCATC
SfcI 462 CTACAG C^T[GA][CT]AG
1821 CTACAG
SfoI 115 GGCGCC GGC^GCC
342 GGCGCC
SmlI 650 CTCAAG C^T[CT][GA]AG
1502 CTCAAG
TaqI 875 TCGA T^CGA
1305 TCGA
TthHB8I 875 TCGA T^CGA
1305 TCGA
XcmI 1382 CCAGAGAGCACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1474 CCAGAACTGACCTGG
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# 3 OR MORE CUT ENZYMES ( 83 enzymes) #
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AccII AciI AcyI AfaI AluI
AocI ApaI AspHI AvaII BanI
BanII BbvI BcnI BmyI BpmI
BsaHI BsaJI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrI BssKI
BstF5I BstNI BstUI BstXI BstYI
Bsu36I Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI Eco81I
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
MaeII MaeIII MboI MboII MflI
MnlI MseI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV PleI RsaI Sau3AI Sau96I
ScrFI StyI Tru9I TseI Tsp45I
Tsp509I TspRI XhoII