################################## # NO CUT ENZYMES ( 123 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AflIII AgeI Alw44I AlwNI Aor51HI ApaLI AscI AseI AsnI Asp718I AspI AvaII AviII BalI BbeI BbrPI BbvCI BciVI BclI BglI BglII BlpI Bpu1102I BsaAI BsaWI BseAI BsiEI BsiWI BsmBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstXI BstZ17I CelII Cfr10I ClaI CpoI DraIII DrdI EaeI EagI EarI EclXI Eco47III Eco52I EcoNI EcoRI EcoT22I FbaI FseI FspI HaeII HpaI KasI KpnI Ksp632I KspI MaeII MfeI MluI MluNI MroI MscI MslI MunI NaeI NarI NdeI NgoMIV NheI NotI NsiI NspV PacI PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SapI SbfI ScaI SfaNI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SwaI Tth111I XbaI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 53 enzymes) # ################################### AcyI 1041 GGCGTC G[GA]^CG[CT]C AflII 207 CTTAAG C^TTAAG AhdI 404 GACGATGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AocI 783 CCTCAGG CC^T[ACGT]AGG ApaI 838 GGGCCC GGGCC^C Asp700I 76 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 404 GACGATGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AvaI 695 CTCGAG C^[CT]CG[GA]G AvrII 825 CCTAGG C^CTAGG BamHI 885 GGATCC G^GATCC BanI 131 GGCACC G^G[CT][GA]CC BbsI 324 GTCTTC GAAGAC(2/6) BcgI 1090 GCAGCAGCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BfrI 207 CTTAAG C^TTAAG BlnI 825 CCTAGG C^CTAGG BpuAI 324 GTCTTC GAAGAC(2/6) BsaBI 625 GATGTAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 1041 GGCGTC G[GA]^CG[CT]C BsaI 308 GAGACC GGTCTC(1/5) BsgI 1067 GTGCAG GTGCAG(16/14) BsmI 714 GAATGC GAATGC(1/-1) BsoBI 695 CTCGAG C^[CT]CG[GA]G BsrBI 915 GAGCGG CCGCTC(-3/-3) BstF5I 1076 GGATG GGATG(2/0) BstYI 885 GGATCC [GA]^GATC[CT] Bsu36I 783 CCTCAGG CC^T[ACGT]AGG DraI 1114 TTTAAA TTT^AAA DsaI 872 CCATGG C^C[GA][CT]GG Eam1105I 404 GACGATGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 783 CCTCAGG CC^T[ACGT]AGG EcoRV 994 GATATC GAT^ATC FauI 313 CCCGC CCCGC(4/6) FokI 1076 GGATG GGATG(9/13) Hin1I 1041 GGCGTC G[GA]^CG[CT]C HincII 601 GTCAAC GT[CT]^[GA]AC HindII 601 GTCAAC GT[CT]^[GA]AC MamI 625 GATGTAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MflI 885 GGATCC [GA]^GATC[CT] MwoI 356 GCACTGTGAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NcoI 872 CCATGG C^CATGG NruI 1055 TCGCGA TCG^CGA NspI 747 GCATGT [GA]CATG^[CT] PaeR7I 695 CTCGAG C^TCGAG PflMI 1005 CCAGTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PstI 1088 CTGCAG CTGCA^G SacI 259 GAGCTC GAGCT^C SspI 721 AATATT AAT^ATT StuI 78 AGGCCT AGG^CCT Van91I 1005 CCAGTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 872 CCATGGAATTTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 695 CTCGAG C^TCGAG XhoII 885 GGATCC [GA]^GATC[CT] XmnI 76 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AccII 928 CGCG CG^CG 1056 CGCG AciI 314 CCGC CCGC(-3/-1) 917 GCGG AfaI 798 GTAC GT^AC 850 GTAC BanII 259 GAGCTC G[GA]GC[CT]^C 838 GGGCCC BfaI 826 CTAG C^TAG 977 CTAG BpmI 351 CTCCAG CTGGAG(16/14) 899 CTGGAG BsiYI 472 CCTAACAATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 1005 CCAGTCTGTGG BslI 472 CCTAACAATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 1005 CCAGTCTGTGG BspMI 830 GCAGGT ACCTGC(4/8) 1069 GCAGGT BstEII 178 GGTGACC G^GT[ACGT]ACC 1101 GGTGACC BstPI 178 GGTGACC G^GT[ACGT]ACC 1101 GGTGACC BstUI 928 CGCG CG^CG 1056 CGCG CfoI 577 GCGC GCG^C 927 GCGC EcoO65I 178 GGTGACC G^GT[ACGT]ACC 1101 GGTGACC HhaI 577 GCGC GCG^C 927 GCGC HinP1I 577 GCGC G^CGC 927 GCGC MaeI 826 CTAG C^TAG 977 CTAG MspA1I 501 CAGCTG C[AC]G^C[GT]G 595 CAGCTG MvnI 928 CGCG CG^CG 1056 CGCG PleI 868 GAGTC GAGTC(4/5) 1026 GAGTC PvuII 501 CAGCTG CAG^CTG 595 CAGCTG RsaI 798 GTAC GT^AC 850 GTAC SexAI 182 ACCTGGT A^CC[AT]GGT 800 ACCTGGT SfcI 498 CTACAG C^T[GA][CT]AG 1088 CTGCAG ######################################### # 3 OR MORE CUT ENZYMES ( 58 enzymes) # ######################################### AcsI AluI AlwI ApoI AspHI BbvI BcnI BmyI BsaJI BseRI BsiHKAI BsmAI BsmFI Bsp1286I BsrI BssKI BstNI Cac8I Cfr13I DdeI DpnII DraII EcoO109I EcoRII EcoT14I Fnu4HI HaeIII HapII HgaI HindIII HinfI HpaII HphI ItaI MaeIII MboI MboII MnlI MseI MspI MvaI NciI NdeII NlaIII NlaIV Sau3AI Sau96I ScrFI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I