Restriction map for
pF1KSDA0839
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# NO CUT ENZYMES ( 78 enzymes) #
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AatII AccIII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI AscI
AspEI AvrII BbrPI BcgI BglI
BlnI BsaAI BsaBI BsaWI BseAI
BsiEI BsiWI BsmBI Bsp1407I BspEI
BsrFI BsrGI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstXI BstZ17I
Bsu36I Cfr10I CpoI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoO65I
EcoRI FseI KspI MamI MluI
MroI NaeI NgoMIV NheI NotI
NruI NspV PacI PinAI PmaCI
PmeI PmlI PshAI PvuI RsrII
SacII SalI SapI SbfI SexAI
SfiI SfuI SgrAI SnaBI Sse8387I
SspBI StuI SwaI
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# ONE CUT ENZYMES ( 51 enzymes) #
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Acc65I 1271 GGTACC G^GTACC
AccI 770 GTCTAC GT^[AC][GT]AC
AccII 1276 CGCG CG^CG
AflII 2038 CTTAAG C^TTAAG
AflIII 3141 ACATGT A^C[GA][CT]GT
AseI 1740 ATTAAT AT^TAAT
AsnI 1740 ATTAAT AT^TAAT
Asp718I 1271 GGTACC G^GTACC
AspI 819 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 3615 GGATCC G^GATCC
BanII 3022 GAGCTC G[GA]GC[CT]^C
BbeI 94 GGCGCC GGCGC^C
BfrI 2038 CTTAAG C^TTAAG
BlpI 2755 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 2755 GCTGAGC GC^T[ACGT]AGC
BsaI 2428 GAGACC GGTCTC(1/5)
BsmI 2532 GCATTC GAATGC(1/-1)
BspDI 2422 ATCGAT AT^CGAT
BspLU11I 3141 ACATGT A^CATGT
BsrBI 2507 CCGCTC CCGCTC(-3/-3)
BstUI 1276 CGCG CG^CG
CelII 2755 GCTGAGC GC^T[ACGT]AGC
ClaI 2422 ATCGAT AT^CGAT
DrdI 3886 GACAAAGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRV 2550 GATATC GAT^ATC
EcoT22I 3563 ATGCAT ATGCA^T
FauI 75 GCGGG CCCGC(4/6)
HaeII 94 GGCGCC [GA]GCGC^[CT]
HindIII 1778 AAGCTT A^AGCTT
HpaI 2923 GTTAAC GTT^AAC
KasI 94 GGCGCC G^GCGCC
KpnI 1271 GGTACC GGTAC^C
MfeI 4058 CAATTG C^AATTG
MunI 4058 CAATTG C^AATTG
MvnI 1276 CGCG CG^CG
NarI 94 GGCGCC GG^CGCC
NdeI 1167 CATATG CA^TATG
NsiI 3563 ATGCAT ATGCA^T
PaeR7I 275 CTCGAG C^TCGAG
PflFI 819 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshBI 1740 ATTAAT AT^TAAT
SacI 3022 GAGCTC GAGCT^C
SfoI 94 GGCGCC GGC^GCC
SmaI 50 CCCGGG CCC^GGG
SpeI 4077 ACTAGT A^CTAGT
SphI 3036 GCATGC GCATG^C
Tth111I 819 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 1147 TCTAGA T^CTAGA
XhoI 275 CTCGAG C^TCGAG
XmaCI 50 CCCGGG C^CCGGG
XmaI 50 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 29 enzymes) #
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AclI 1606 AACGTT AA^CGTT
1805 AACGTT
Asp700I 1738 GAATTAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3174 GAATACGTTC
AvaI 50 CCCGGG C^[CT]CG[GA]G
275 CTCGAG
AviII 2471 TGCGCA TGC^GCA
2520 TGCGCA
BalI 2417 TGGCCA TGG^CCA
3754 TGGCCA
BbvCI 87 CCTCAGC CCTCAGC(-5/-2)
2296 CCTCAGC
BclI 795 TGATCA T^GATCA
834 TGATCA
BglII 1562 AGATCT A^GATCT
3323 AGATCT
BseRI 79 GAGGAG GAGGAG(10/8)
432 GAGGAG
BsgI 2543 CTGCAC GTGCAG(16/14)
3665 GTGCAG
BsoBI 50 CCCGGG C^[CT]CG[GA]G
275 CTCGAG
BspHI 3503 TCATGA T^CATGA
3882 TCATGA
BstAPI 706 GCAAAAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2463 GCAGCAGATGC
EaeI 2417 TGGCCA [CT]^GGCC[GA]
3754 TGGCCA
FbaI 795 TGATCA T^GATCA
834 TGATCA
FspI 2471 TGCGCA TGC^GCA
2520 TGCGCA
HapII 51 CCGG C^CGG
596 CCGG
HincII 2923 GTTAAC GT[CT]^[GA]AC
3847 GTTGAC
HindII 2923 GTTAAC GT[CT]^[GA]AC
3847 GTTGAC
HpaII 51 CCGG C^CGG
596 CCGG
MluNI 2417 TGGCCA TGG^CCA
3754 TGGCCA
MscI 2417 TGGCCA TGG^CCA
3754 TGGCCA
MspI 51 CCGG C^CGG
596 CCGG
NcoI 1155 CCATGG C^CATGG
2455 CCATGG
Psp1406I 1606 AACGTT AA^CGTT
1805 AACGTT
RcaI 3503 TCATGA T^CATGA
3882 TCATGA
ScaI 552 AGTACT AGT^ACT
2651 AGTACT
SspI 2144 AATATT AAT^ATT
3129 AATATT
XmnI 1738 GAATTAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3174 GAATACGTTC
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# 3 OR MORE CUT ENZYMES ( 100 enzymes) #
#########################################
AciI AcsI AcyI AfaI AluI
AlwI AlwNI ApoI AspHI AvaII
BanI BbsI BbvI BciVI BcnI
BfaI BmyI BpmI BpuAI BsaHI
BsaJI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BspMI BsrDI BsrI
BssKI BstF5I BstNI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraI
DraII DraIII DsaI EarI EcoNI
EcoO109I EcoRII EcoT14I Fnu4HI FokI
HaeIII HgaI HhaI Hin1I HinP1I
HinfI HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MvaI
MwoI NciI NdeII NlaIII NlaIV
NspI PflMI PleI PpuMI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XhoII