Restriction map for
pF1KSDA0841
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# NO CUT ENZYMES ( 109 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AflII AgeI AhdI AocI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvrII BbrPI
BciVI BclI BfrI BglII BlnI
BsaAI BsaBI BsaWI BseAI BsiWI
BsmI Bsp1407I BspDI BspEI BspLU11I
BsrDI BsrGI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstXI BstZ17I
Bsu36I ClaI CpoI DraI DraIII
EagI Eam1105I EclXI Eco52I Eco81I
EcoO65I EcoRI EcoT22I FbaI FseI
HincII HindII HindIII HpaI HphI
KpnI KspI MamI MfeI MroI
MseI MunI NdeI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RsrII SacII
SalI SapI ScaI SexAI SfuI
SgrAI SnaBI SpeI SphI SspBI
SspI SwaI Tru9I Tsp509I Tth111I
XbaI XcmI XhoI XmnI
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# ONE CUT ENZYMES ( 30 enzymes) #
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AfaI 669 GTAC GT^AC
AflIII 993 ACGCGT A^C[GA][CT]GT
Alw44I 148 GTGCAC G^TGCAC
Aor51HI 1482 AGCGCT AGC^GCT
ApaLI 148 GTGCAC G^TGCAC
AviII 92 TGCGCA TGC^GCA
BalI 198 TGGCCA TGG^CCA
BbvCI 1125 GCTGAGG CCTCAGC(-5/-2)
BcgI 565 CGAACAGCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsiEI 41 CGGTCG CG[GA][CT]^CG
BspHI 645 TCATGA T^CATGA
BspMI 1017 GCAGGT ACCTGC(4/8)
BsrFI 1536 GCCGGC [GA]^CCGG[CT]
Cfr10I 1536 GCCGGC [GA]^CCGG[CT]
DrdI 855 GACCCTGCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1482 AGCGCT AGC^GCT
EcoRV 813 GATATC GAT^ATC
FspI 92 TGCGCA TGC^GCA
MluI 993 ACGCGT A^CGCGT
MluNI 198 TGGCCA TGG^CCA
MscI 198 TGGCCA TGG^CCA
MslI 327 CACGGCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 1536 GCCGGC GCC^GGC
NgoMIV 1536 GCCGGC G^CCGGC
NspI 144 GCATGT [GA]CATG^[CT]
PleI 844 GACTC GAGTC(4/5)
PpuMI 1559 AGGACCT [GA]G^G[AT]CC[CT]
RcaI 645 TCATGA T^CATGA
RsaI 669 GTAC GT^AC
SfiI 1869 GGCCCAGGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
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# TWO CUT ENZYMES ( 30 enzymes) #
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AcyI 464 GGCGCC G[GA]^CG[CT]C
1166 GGCGCC
AvaI 71 CCCGGG C^[CT]CG[GA]G
1251 CCCGGG
BbeI 464 GGCGCC GGCGC^C
1166 GGCGCC
BbsI 410 GAAGAC GAAGAC(2/6)
1617 GAAGAC
BfaI 7 CTAG C^TAG
1264 CTAG
BlpI 681 GCTGAGC GC^T[ACGT]AGC
1485 GCTGAGC
Bpu1102I 681 GCTGAGC GC^T[ACGT]AGC
1485 GCTGAGC
BpuAI 410 GAAGAC GAAGAC(2/6)
1617 GAAGAC
BsaHI 464 GGCGCC G[GA]^CG[CT]C
1166 GGCGCC
BsaI 1183 GAGACC GGTCTC(1/5)
912 GGTCTC
BsmBI 694 GAGACG CGTCTC(1/5)
990 GAGACG
BsoBI 71 CCCGGG C^[CT]CG[GA]G
1251 CCCGGG
BsrBI 757 GAGCGG CCGCTC(-3/-3)
940 GAGCGG
CelII 681 GCTGAGC GC^T[ACGT]AGC
1485 GCTGAGC
Hin1I 464 GGCGCC G[GA]^CG[CT]C
1166 GGCGCC
KasI 464 GGCGCC G^GCGCC
1166 GGCGCC
MaeI 7 CTAG C^TAG
1264 CTAG
MaeIII 250 GTGAC ^GT[ACGT]AC
502 GTGAC
NarI 464 GGCGCC GG^CGCC
1166 GGCGCC
NcoI 332 CCATGG C^CATGG
866 CCATGG
NheI 6 GCTAGC G^CTAGC
1263 GCTAGC
SbfI 468 CCTGCAGG CCTGCA^GG
1572 CCTGCAGG
SfoI 464 GGCGCC GGC^GCC
1166 GGCGCC
SmaI 71 CCCGGG CCC^GGG
1251 CCCGGG
SmlI 356 CTCAAG C^T[CT][GA]AG
1063 CTCAAG
Sse8387I 468 CCTGCAGG CCTGCA^GG
1572 CCTGCAGG
StuI 1517 AGGCCT AGG^CCT
1635 AGGCCT
Tsp45I 250 GTGAC ^GT[GC]AC
502 GTGAC
XmaCI 71 CCCGGG C^CCGGG
1251 CCCGGG
XmaI 71 CCCGGG C^CCGGG
1251 CCCGGG
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# 3 OR MORE CUT ENZYMES ( 89 enzymes) #
#########################################
AccII AciI AluI AlwI AlwNI
ApaI AspHI AvaII BamHI BanI
BanII BbvI BcnI BglI BmyI
BpmI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrI BssKI BstAPI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI HinP1I HinfI
HpaII ItaI Ksp632I MaeII MboI
MboII MflI MnlI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV PflMI PstI PvuII
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI StyI TaqI TfiI TseI
TspRI TthHB8I Van91I XhoII