Restriction map for
pF1KSDA0863
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# NO CUT ENZYMES ( 100 enzymes) #
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AatII Acc65I AccII AclI AcyI
AflII AgeI ApaI AscI AseI
AsnI Asp700I Asp718I AspI AviII
AvrII BamHI BbeI BbrPI BclI
BfrI BlnI BlpI Bpu1102I BsaAI
BsaHI BsiEI BsiWI BsmBI Bsp1407I
BspDI BspHI BsrGI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstUI
BstZ17I CelII ClaI CpoI DraIII
DsaI EagI EclXI Eco52I EcoNI
EcoO65I FbaI FseI FspI HgaI
Hin1I HincII HindII HpaI KasI
KpnI KspI MfeI MluI MunI
MvnI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NspV
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshBI Psp1406I PvuI RcaI
RsrII SacII SalI ScaI SfiI
SfoI SfuI SgrAI SnaBI SspBI
SwaI Tth111I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 38 enzymes) #
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AccIII 392 TCCGGA T^CCGGA
AflIII 466 ACATGT A^C[GA][CT]GT
AhdI 3083 GACCTATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 2204 GTGCAC G^TGCAC
AocI 2581 CCTCAGG CC^T[ACGT]AGG
Aor51HI 850 AGCGCT AGC^GCT
ApaLI 2204 GTGCAC G^TGCAC
AspEI 3083 GACCTATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BcgI 3035 CGAGTGTTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 2537 GGATAC GTATCC(6/5)
BglI 1674 GCCAGTGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 742 AGATCT A^GATCT
BsaBI 1914 GATGCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 392 TCCGGA [AT]^CCGG[AT]
BseAI 392 TCCGGA T^CCGGA
BsmI 1998 GAATGC GAATGC(1/-1)
BspEI 392 TCCGGA T^CCGGA
BspLU11I 466 ACATGT A^CATGT
BsrBI 2506 GAGCGG CCGCTC(-3/-3)
BsrDI 795 CATTGC GCAATG(2/0)
Bsu36I 2581 CCTCAGG CC^T[ACGT]AGG
DraI 3049 TTTAAA TTT^AAA
DrdI 1473 GACAGCTCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 3083 GACCTATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 850 AGCGCT AGC^GCT
Eco81I 2581 CCTCAGG CC^T[ACGT]AGG
EcoRI 1614 GAATTC G^AATTC
EcoT14I 2825 CCAAGG C^C[AT][AT]GG
HaeII 850 AGCGCT [GA]GCGC^[CT]
MaeII 2117 ACGT A^CGT
MamI 1914 GATGCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 392 TCCGGA T^CCGGA
PacI 544 TTAATTAA TTAAT^TAA
PshAI 993 GACTCTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 2101 GAGCTC GAGCT^C
SpeI 1159 ACTAGT A^CTAGT
SphI 1973 GCATGC GCATG^C
StyI 2825 CCAAGG C^C[AT][AT]GG
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# TWO CUT ENZYMES ( 30 enzymes) #
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AcsI 8 AAATTC [GA]^AATT[CT]
1614 GAATTC
AfaI 498 GTAC GT^AC
3325 GTAC
AlwNI 923 CAGTGACTG CAG[ACGT][ACGT][ACGT]^CTG
1924 CAGCTCCTG
ApoI 8 AAATTC [GA]^AATT[CT]
1614 GAATTC
AvaI 2599 CCCGGG C^[CT]CG[GA]G
3015 CCCGGG
BalI 978 TGGCCA TGG^CCA
1434 TGGCCA
BanII 1263 GAGCCC G[GA]GC[CT]^C
2101 GAGCTC
BbsI 2079 GAAGAC GAAGAC(2/6)
2962 GAAGAC
BpuAI 2079 GAAGAC GAAGAC(2/6)
2962 GAAGAC
BsoBI 2599 CCCGGG C^[CT]CG[GA]G
3015 CCCGGG
BstAPI 2197 GCAGCGTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2744 GCATCCACTGC
EarI 3104 CTCTTC CTCTTC(1/4)
2275 GAAGAG
EcoRV 200 GATATC GAT^ATC
2341 GATATC
EcoT22I 2299 ATGCAT ATGCA^T
2905 ATGCAT
FauI 2731 CCCGC CCCGC(4/6)
2508 GCGGG
HindIII 736 AAGCTT A^AGCTT
2497 AAGCTT
Ksp632I 3104 CTCTTC CTCTTC(1/4)
2275 GAAGAG
MluNI 978 TGGCCA TGG^CCA
1434 TGGCCA
MscI 978 TGGCCA TGG^CCA
1434 TGGCCA
NsiI 2299 ATGCAT ATGCA^T
2905 ATGCAT
RsaI 498 GTAC GT^AC
3325 GTAC
SapI 2275 GAAGAGC GCTCTTC(1/4)
3103 GCTCTTC
SmaI 2599 CCCGGG CCC^GGG
3015 CCCGGG
SmlI 1119 CTTGAG C^T[CT][GA]AG
2179 CTTGAG
SspI 418 AATATT AAT^ATT
641 AATATT
TaqI 204 TCGA T^CGA
2435 TCGA
TthHB8I 204 TCGA T^CGA
2435 TCGA
XcmI 936 CCAGGGTGCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2209 CCATTTCTAAAGTGG
XmaCI 2599 CCCGGG C^CCGGG
3015 CCCGGG
XmaI 2599 CCCGGG C^CCGGG
3015 CCCGGG
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# 3 OR MORE CUT ENZYMES ( 90 enzymes) #
#########################################
AccI AciI AluI AlwI AspHI
AvaII BanI BbvCI BbvI BcnI
BfaI BmyI BpmI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BspMI BsrFI
BsrI BssKI BstF5I BstNI BstXI
BstYI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII EaeI EcoO109I
EcoRII Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI MaeI MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV NspI PflMI PleI
PpuMI PstI PvuII Sau3AI Sau96I
SbfI ScrFI SexAI SfaNI SfcI
Sse8387I StuI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI Van91I XhoII