Restriction map for pF1KSDA0875
##################################
# NO CUT ENZYMES (   99 enzymes) #
##################################

AatII       Acc65I      AclI        AcyI        AflIII      
AgeI        Alw44I      AocI        Aor51HI     ApaI        
ApaLI       AscI        AseI        AsnI        Asp700I     
Asp718I     AviII       AvrII       BbeI        BbsI        
BcgI        BclI        BlnI        BlpI        Bpu1102I    
BpuAI       BsaHI       BsaI        BsiWI       BsmBI       
Bsp1407I    BspDI       BspHI       BspLU11I    BsrBI       
BsrGI       BssHII      Bst1107I    BstAPI      BstBI       
BstEII      BstPI       BstZ17I     Bsu36I      CelII       
ClaI        CpoI        DraI        DraIII      DrdI        
DsaI        Eco47III    Eco81I      EcoNI       EcoO65I     
EcoRI       EcoRV       FbaI        FseI        FspI        
Hin1I       HpaI        KasI        KpnI        KspI        
MluI        NarI        NcoI        NdeI        NheI        
NotI        NruI        NspV        PacI        PaeR7I      
PinAI       PmeI        PshAI       PshBI       Psp1406I    
PvuI        RcaI        RsrII       SacI        SacII       
SapI        SbfI        SfiI        SfoI        SfuI        
SgrAI       SnaBI       SpeI        SphI        Sse8387I    
SspBI       SwaI        XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   58 enzymes) #
###################################

AccI           16  GTCGAC               GT^[AC][GT]AC
AccIII       1580  TCCGGA               T^CCGGA
AcsI          503  AAATTC               [GA]^AATT[CT]
AflII         544  CTTAAG               C^TTAAG
AhdI          571  GACTATGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApoI          503  AAATTC               [GA]^AATT[CT]
AspEI         571  GACTATGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI          274  GACTACGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaI         1362  CCCGGG               C^[CT]CG[GA]G
BalI         1315  TGGCCA               TGG^CCA
BamHI        1577  GGATCC               G^GATCC
BanII        1495  GGGCTC               G[GA]GC[CT]^C
BbrPI        1108  CACGTG               CAC^GTG
BbvCI          78  CCTCAGC              CCTCAGC(-5/-2)
BfrI          544  CTTAAG               C^TTAAG
BglI          727  GCCTTCAAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI        1108  CACGTG               [CT]AC^GT[GA]
BsaBI        1237  GATCTCTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI        1580  TCCGGA               T^CCGGA
BsmI         1084  GAATGC               GAATGC(1/-1)
BsoBI        1362  CCCGGG               C^[CT]CG[GA]G
BspEI        1580  TCCGGA               T^CCGGA
BsrDI        1249  GCAATG               GCAATG(2/0)
BssSI        1570  CACGAG               CACGAG(-5/-1)
BstXI         749  CCATGATAATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraII        1547  GGGACCC              [GA]G^G[ACGT]CC[CT]
Eam1105I      571  GACTATGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI          292  GAAGAG               CTCTTC(1/4)
EcoO109I     1547  GGGACCC              [GA]G^G[ACGT]CC[CT]
EcoT14I       999  CCAAGG               C^C[AT][AT]GG
EcoT22I       868  ATGCAT               ATGCA^T
HaeII          42  GGCGCT               [GA]GCGC^[CT]
HgaI         1727  GACGC                GACGC(5/10)
HindIII       473  AAGCTT               A^AGCTT
Ksp632I       292  GAAGAG               CTCTTC(1/4)
MamI         1237  GATCTCTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI        1315  TGGCCA               TGG^CCA
MroI         1580  TCCGGA               T^CCGGA
MscI         1315  TGGCCA               TGG^CCA
NsiI          868  ATGCAT               ATGCA^T
NspI          908  GCATGT               [GA]CATG^[CT]
PflFI         274  GACTACGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI         777  CCAGGTGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI        1108  CACGTG               CAC^GTG
PmlI         1108  CACGTG               CAC^GTG
PpuMI        1547  GGGACCC              [GA]G^G[AT]CC[CT]
PstI         1115  CTGCAG               CTGCA^G
SalI           16  GTCGAC               G^TCGAC
SmaI         1362  CCCGGG               CCC^GGG
SspI         1828  AATATT               AAT^ATT
StuI          548  AGGCCT               AGG^CCT
StyI          999  CCAAGG               C^C[AT][AT]GG
Tth111I       274  GACTACGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Van91I        777  CCAGGTGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI         1404  TCTAGA               T^CTAGA
XcmI         1553  CCACCTGCATGATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI        1362  CCCGGG               C^CCGGG
XmaI         1362  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

AspHI         370  GAGCAC               G[AT]GC[AT]^C
             1408  GAGCAC              
BanI           36  GGTGCC               G^G[CT][GA]CC
              995  GGTGCC              
BciVI         449  GTATCC               GTATCC(6/5)
             1788  GTATCC              
BfaI         1357  CTAG                 C^TAG
             1405  CTAG                
BglII         530  AGATCT               A^GATCT
             1797  AGATCT              
BsaWI        1580  TCCGGA               [AT]^CCGG[AT]
             1784  TCCGGT              
BsiEI         143  CGGCCG               CG[GA][CT]^CG
              156  CGGCCG              
BsiHKAI       370  GAGCAC               G[AT]GC[AT]^C
             1408  GAGCAC              
BsrFI          39  GCCGGC               [GA]^CCGG[CT]
              176  GCCGGC              
Cfr10I         39  GCCGGC               [GA]^CCGG[CT]
              176  GCCGGC              
EagI          143  CGGCCG               C^GGCCG
              156  CGGCCG              
EclXI         143  CGGCCG               C^GGCCG
              156  CGGCCG              
Eco52I        143  CGGCCG               C^GGCCG
              156  CGGCCG              
MaeI         1357  CTAG                 C^TAG
             1405  CTAG                
MfeI          282  CAATTG               C^AATTG
              929  CAATTG              
MunI          282  CAATTG               C^AATTG
              929  CAATTG              
NaeI           39  GCCGGC               GCC^GGC
              176  GCCGGC              
NgoMIV         39  GCCGGC               G^CCGGC
              176  GCCGGC              
PvuII          81  CAGCTG               CAG^CTG
             1069  CAGCTG              
ScaI          611  AGTACT               AGT^ACT
             1091  AGTACT              
SexAI        1262  ACCAGGT              A^CC[AT]GGT
             1388  ACCTGGT             
SfcI          261  CTACAG               C^T[GA][CT]AG
             1115  CTGCAG              
SmlI          544  CTTAAG               C^T[CT][GA]AG
             1700  CTCAAG              


#########################################
# 3 OR MORE CUT ENZYMES (   78 enzymes) #
#########################################

AccII       AciI        AfaI        AluI        AlwI        
AlwNI       AvaII       BbvI        BcnI        BmyI        
BpmI        BsaJI       BseRI       BsgI        BsiYI       
BslI        BsmAI       BsmFI       Bsp1286I    BspMI       
BsrI        BssKI       BstF5I      BstNI       BstUI       
BstYI       Cac8I       CfoI        Cfr13I      DdeI        
DpnII       EaeI        EcoRII      FauI        Fnu4HI      
FokI        HaeIII      HapII       HhaI        HinP1I      
HincII      HindII      HinfI       HpaII       HphI        
ItaI        MaeII       MaeIII      MboI        MboII       
MflI        MnlI        MseI        MslI        MspA1I      
MspI        MvaI        MvnI        MwoI        NciI        
NdeII       NlaIII      NlaIV       PleI        RsaI        
Sau3AI      Sau96I      ScrFI       SfaNI       TaqI        
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       TthHB8I     XhoII       
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