################################## # NO CUT ENZYMES ( 99 enzymes) # ################################## AatII Acc65I AclI AcyI AflIII AgeI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AviII AvrII BbeI BbsI BcgI BclI BlnI BlpI Bpu1102I BpuAI BsaHI BsaI BsiWI BsmBI Bsp1407I BspDI BspHI BspLU11I BsrBI BsrGI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstZ17I Bsu36I CelII ClaI CpoI DraI DraIII DrdI DsaI Eco47III Eco81I EcoNI EcoO65I EcoRI EcoRV FbaI FseI FspI Hin1I HpaI KasI KpnI KspI MluI NarI NcoI NdeI NheI NotI NruI NspV PacI PaeR7I PinAI PmeI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SapI SbfI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SwaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 58 enzymes) # ################################### AccI 16 GTCGAC GT^[AC][GT]AC AccIII 1580 TCCGGA T^CCGGA AcsI 503 AAATTC [GA]^AATT[CT] AflII 544 CTTAAG C^TTAAG AhdI 571 GACTATGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC ApoI 503 AAATTC [GA]^AATT[CT] AspEI 571 GACTATGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 274 GACTACGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 1362 CCCGGG C^[CT]CG[GA]G BalI 1315 TGGCCA TGG^CCA BamHI 1577 GGATCC G^GATCC BanII 1495 GGGCTC G[GA]GC[CT]^C BbrPI 1108 CACGTG CAC^GTG BbvCI 78 CCTCAGC CCTCAGC(-5/-2) BfrI 544 CTTAAG C^TTAAG BglI 727 GCCTTCAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaAI 1108 CACGTG [CT]AC^GT[GA] BsaBI 1237 GATCTCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BseAI 1580 TCCGGA T^CCGGA BsmI 1084 GAATGC GAATGC(1/-1) BsoBI 1362 CCCGGG C^[CT]CG[GA]G BspEI 1580 TCCGGA T^CCGGA BsrDI 1249 GCAATG GCAATG(2/0) BssSI 1570 CACGAG CACGAG(-5/-1) BstXI 749 CCATGATAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG DraII 1547 GGGACCC [GA]G^G[ACGT]CC[CT] Eam1105I 571 GACTATGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 292 GAAGAG CTCTTC(1/4) EcoO109I 1547 GGGACCC [GA]G^G[ACGT]CC[CT] EcoT14I 999 CCAAGG C^C[AT][AT]GG EcoT22I 868 ATGCAT ATGCA^T HaeII 42 GGCGCT [GA]GCGC^[CT] HgaI 1727 GACGC GACGC(5/10) HindIII 473 AAGCTT A^AGCTT Ksp632I 292 GAAGAG CTCTTC(1/4) MamI 1237 GATCTCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluNI 1315 TGGCCA TGG^CCA MroI 1580 TCCGGA T^CCGGA MscI 1315 TGGCCA TGG^CCA NsiI 868 ATGCAT ATGCA^T NspI 908 GCATGT [GA]CATG^[CT] PflFI 274 GACTACGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 777 CCAGGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 1108 CACGTG CAC^GTG PmlI 1108 CACGTG CAC^GTG PpuMI 1547 GGGACCC [GA]G^G[AT]CC[CT] PstI 1115 CTGCAG CTGCA^G SalI 16 GTCGAC G^TCGAC SmaI 1362 CCCGGG CCC^GGG SspI 1828 AATATT AAT^ATT StuI 548 AGGCCT AGG^CCT StyI 999 CCAAGG C^C[AT][AT]GG Tth111I 274 GACTACGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 777 CCAGGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 1404 TCTAGA T^CTAGA XcmI 1553 CCACCTGCATGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 1362 CCCGGG C^CCGGG XmaI 1362 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AspHI 370 GAGCAC G[AT]GC[AT]^C 1408 GAGCAC BanI 36 GGTGCC G^G[CT][GA]CC 995 GGTGCC BciVI 449 GTATCC GTATCC(6/5) 1788 GTATCC BfaI 1357 CTAG C^TAG 1405 CTAG BglII 530 AGATCT A^GATCT 1797 AGATCT BsaWI 1580 TCCGGA [AT]^CCGG[AT] 1784 TCCGGT BsiEI 143 CGGCCG CG[GA][CT]^CG 156 CGGCCG BsiHKAI 370 GAGCAC G[AT]GC[AT]^C 1408 GAGCAC BsrFI 39 GCCGGC [GA]^CCGG[CT] 176 GCCGGC Cfr10I 39 GCCGGC [GA]^CCGG[CT] 176 GCCGGC EagI 143 CGGCCG C^GGCCG 156 CGGCCG EclXI 143 CGGCCG C^GGCCG 156 CGGCCG Eco52I 143 CGGCCG C^GGCCG 156 CGGCCG MaeI 1357 CTAG C^TAG 1405 CTAG MfeI 282 CAATTG C^AATTG 929 CAATTG MunI 282 CAATTG C^AATTG 929 CAATTG NaeI 39 GCCGGC GCC^GGC 176 GCCGGC NgoMIV 39 GCCGGC G^CCGGC 176 GCCGGC PvuII 81 CAGCTG CAG^CTG 1069 CAGCTG ScaI 611 AGTACT AGT^ACT 1091 AGTACT SexAI 1262 ACCAGGT A^CC[AT]GGT 1388 ACCTGGT SfcI 261 CTACAG C^T[GA][CT]AG 1115 CTGCAG SmlI 544 CTTAAG C^T[CT][GA]AG 1700 CTCAAG ######################################### # 3 OR MORE CUT ENZYMES ( 78 enzymes) # ######################################### AccII AciI AfaI AluI AlwI AlwNI AvaII BbvI BcnI BmyI BpmI BsaJI BseRI BsgI BsiYI BslI BsmAI BsmFI Bsp1286I BspMI BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr13I DdeI DpnII EaeI EcoRII FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HincII HindII HinfI HpaII HphI ItaI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PleI RsaI Sau3AI Sau96I ScrFI SfaNI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII