Restriction map for
pF1KSDA0881
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# NO CUT ENZYMES ( 88 enzymes) #
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Acc65I AclI AgeI Aor51HI AscI
AseI AsnI Asp718I AspI AviII
AvrII BamHI BbeI BbrPI BciVI
BclI BglII BlnI BsaAI BsiEI
BsiWI Bsp1407I BspDI BspHI BspLU11I
BsrGI BssHII BstBI BstEII BstPI
BstXI ClaI CpoI DraI DrdI
EagI EclXI Eco47III Eco52I EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HindIII HpaI KasI KpnI
MfeI MluI MunI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PflFI PflMI
PinAI PmaCI PmeI PmlI PshBI
Psp1406I PvuI RcaI RsrII SbfI
SexAI SfiI SfoI SfuI SgrAI
SnaBI SpeI Sse8387I SspBI SspI
SwaI Tth111I Van91I
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# ONE CUT ENZYMES ( 35 enzymes) #
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AatII 619 GACGTC GACGT^C
AcsI 765 GAATTT [GA]^AATT[CT]
AflII 2353 CTTAAG C^TTAAG
AflIII 2269 ACGTGT A^C[GA][CT]GT
AhdI 619 GACGTCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 285 CCTGAGG CC^T[ACGT]AGG
ApoI 765 GAATTT [GA]^AATT[CT]
Asp700I 2253 GAAGCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 619 GACGTCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbsI 782 GTCTTC GAAGAC(2/6)
BbvCI 1536 GCTGAGG CCTCAGC(-5/-2)
BcgI 3098 GCACCAGTGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 2353 CTTAAG C^TTAAG
BglI 3012 GCCCCTGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 782 GTCTTC GAAGAC(2/6)
BsaBI 2538 GATCAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmI 684 GAATGC GAATGC(1/-1)
BsrDI 708 CATTGC GCAATG(2/0)
BsrFI 1250 ACCGGC [GA]^CCGG[CT]
Bst1107I 124 GTATAC GTA^TAC
BstZ17I 124 GTATAC GTA^TAC
Bsu36I 285 CCTGAGG CC^T[ACGT]AGG
Cfr10I 1250 ACCGGC [GA]^CCGG[CT]
Eam1105I 619 GACGTCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 285 CCTGAGG CC^T[ACGT]AGG
KspI 3071 CCGCGG CCGC^GG
MamI 2538 GATCAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MslI 445 CATAGGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
PaeR7I 1042 CTCGAG C^TCGAG
SacII 3071 CCGCGG CCGC^GG
SalI 772 GTCGAC G^TCGAC
StuI 2711 AGGCCT AGG^CCT
XbaI 345 TCTAGA T^CTAGA
XhoI 1042 CTCGAG C^TCGAG
XmnI 2253 GAAGCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 22 enzymes) #
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AccI 124 GTATAC GT^[AC][GT]AC
772 GTCGAC
AccIII 143 TCCGGA T^CCGGA
761 TCCGGA
AcyI 619 GACGTC G[GA]^CG[CT]C
2900 GGCGTC
BsaHI 619 GACGTC G[GA]^CG[CT]C
2900 GGCGTC
BsaWI 143 TCCGGA [AT]^CCGG[AT]
761 TCCGGA
BseAI 143 TCCGGA T^CCGGA
761 TCCGGA
BsgI 424 CTGCAC GTGCAG(16/14)
2092 GTGCAG
BsmBI 2909 CGTCTC CGTCTC(1/5)
2266 GAGACG
BspEI 143 TCCGGA T^CCGGA
761 TCCGGA
BstAPI 1660 GCACGAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2175 GCAAGAGTTGC
Hin1I 619 GACGTC G[GA]^CG[CT]C
2900 GGCGTC
HincII 772 GTCGAC GT[CT]^[GA]AC
1102 GTCAAC
HindII 772 GTCGAC GT[CT]^[GA]AC
1102 GTCAAC
MaeII 620 ACGT A^CGT
2269 ACGT
MroI 143 TCCGGA T^CCGGA
761 TCCGGA
MseI 677 TTAA T^TAA
2354 TTAA
PshAI 743 GACACTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
775 GACTCCTGTC
ScaI 755 AGTACT AGT^ACT
1910 AGTACT
SphI 2193 GCATGC GCATG^C
2882 GCATGC
TfiI 721 GAATC G^A[AT]TC
2672 GAATC
Tru9I 677 TTAA T^TAA
2354 TTAA
Tsp509I 99 AATT ^AATT
766 AATT
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# 3 OR MORE CUT ENZYMES ( 113 enzymes) #
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AccII AciI AfaI AluI Alw44I
AlwI AlwNI ApaI ApaLI AspHI
AvaI AvaII BalI BanI BanII
BbvI BcnI BfaI BlpI BmyI
BpmI Bpu1102I BsaI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrBI BsrI
BssKI BssSI BstF5I BstNI BstUI
BstYI Cac8I CelII CfoI Cfr13I
DdeI DpnII DraII DraIII DsaI
EaeI EarI EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeIII MboI MboII MflI
MluNI MnlI MscI MspA1I MspI
MvaI MvnI MwoI NciI NcoI
NdeII NlaIII NlaIV NspI PleI
PpuMI PstI PvuII RsaI SacI
SapI Sau3AI Sau96I ScrFI SfaNI
SfcI SmaI SmlI StyI TaqI
TseI Tsp45I TspRI TthHB8I XcmI
XhoII XmaCI XmaI