Restriction map for
pF1KSDA0899
##################################
# NO CUT ENZYMES ( 79 enzymes) #
##################################
AccIII AgeI AhdI Aor51HI AscI
AseI AsnI AspEI AspI AvrII
BamHI BbrPI BbvCI BcgI BlnI
BsaWI BseAI BsiWI BspDI BspEI
BspLU11I BsrDI Bst1107I BstBI BstEII
BstPI BstZ17I ClaI CpoI DraIII
Eam1105I Eco47III EcoNI EcoO65I EcoRI
EcoT22I FseI HindIII HpaI MfeI
MluI MroI MunI NdeI NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshBI PvuI RsrII SacI
SalI SbfI ScaI SfiI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspI SwaI Tth111I XbaI
XcmI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
Acc65I 1382 GGTACC G^GTACC
AccI 1956 GTCTAC GT^[AC][GT]AC
AclI 2516 AACGTT AA^CGTT
AflII 2179 CTTAAG C^TTAAG
AocI 454 CCTAAGG CC^T[ACGT]AGG
Asp718I 1382 GGTACC G^GTACC
BbeI 2394 GGCGCC GGCGC^C
BciVI 1326 GGATAC GTATCC(6/5)
BfaI 692 CTAG C^TAG
BfrI 2179 CTTAAG C^TTAAG
BglI 1282 GCCATCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 726 AGATCT A^GATCT
BsaAI 1363 TACGTG [CT]AC^GT[GA]
BsmBI 1108 GAGACG CGTCTC(1/5)
BsmI 915 GAATGC GAATGC(1/-1)
Bsp1407I 524 TGTACA T^GTACA
BspHI 207 TCATGA T^CATGA
BsrGI 524 TGTACA T^GTACA
BstAPI 2544 GCAGGAAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 454 CCTAAGG CC^T[ACGT]AGG
DrdI 2584 GACACAGAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 5 CGGCCG C^GGCCG
EclXI 5 CGGCCG C^GGCCG
Eco52I 5 CGGCCG C^GGCCG
Eco81I 454 CCTAAGG CC^T[ACGT]AGG
EcoRV 55 GATATC GAT^ATC
HincII 2362 GTCAAC GT[CT]^[GA]AC
HindII 2362 GTCAAC GT[CT]^[GA]AC
KasI 2394 GGCGCC G^GCGCC
KpnI 1382 GGTACC GGTAC^C
KspI 2012 CCGCGG CCGC^GG
MaeI 692 CTAG C^TAG
NaeI 3 GCCGGC GCC^GGC
NarI 2394 GGCGCC GG^CGCC
NcoI 551 CCATGG C^CATGG
NgoMIV 3 GCCGGC G^CCGGC
NspI 1052 GCATGT [GA]CATG^[CT]
PflMI 594 CCAGCACTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 2177 GACTTAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 2516 AACGTT AA^CGTT
RcaI 207 TCATGA T^CATGA
SacII 2012 CCGCGG CCGC^GG
SfoI 2394 GGCGCC GGC^GCC
SspBI 524 TGTACA T^GTACA
StuI 434 AGGCCT AGG^CCT
Van91I 594 CCAGCACTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AatII 531 GACGTC GACGT^C
711 GACGTC
AflIII 2057 ACGTGT A^C[GA][CT]GT
2441 ACGTGT
Alw44I 577 GTGCAC G^TGCAC
1056 GTGCAC
AlwNI 812 CAGACCCTG CAG[ACGT][ACGT][ACGT]^CTG
1003 CAGTTTCTG
ApaI 1857 GGGCCC GGGCC^C
2016 GGGCCC
ApaLI 577 GTGCAC G^TGCAC
1056 GTGCAC
Asp700I 1644 GAACCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2556 GAACATCTTC
AspHI 577 GTGCAC G[AT]GC[AT]^C
1056 GTGCAC
AviII 1685 TGCGCA TGC^GCA
2759 TGCGCA
BanII 1857 GGGCCC G[GA]GC[CT]^C
2016 GGGCCC
BclI 332 TGATCA T^GATCA
629 TGATCA
BsiHKAI 577 GTGCAC G[AT]GC[AT]^C
1056 GTGCAC
BsrBI 1135 GAGCGG CCGCTC(-3/-3)
1816 GAGCGG
BsrFI 3 GCCGGC [GA]^CCGG[CT]
2747 ACCGGC
BssHII 1610 GCGCGC G^CGCGC
2342 GCGCGC
BssSI 1474 CACGAG CACGAG(-5/-1)
2050 CTCGTG
BstXI 374 CCACCTTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2312 CCAAGCCCGTGG
Cfr10I 3 GCCGGC [GA]^CCGG[CT]
2747 ACCGGC
DraI 130 TTTAAA TTT^AAA
664 TTTAAA
FbaI 332 TGATCA T^GATCA
629 TGATCA
FspI 1685 TGCGCA TGC^GCA
2759 TGCGCA
HaeII 1948 AGCGCC [GA]GCGC^[CT]
2394 GGCGCC
HgaI 1098 GACGC GACGC(5/10)
1598 GCGTC
PpuMI 458 AGGTCCT [GA]G^G[AT]CC[CT]
1920 GGGTCCT
SapI 186 GCTCTTC GCTCTTC(1/4)
1453 GCTCTTC
SexAI 1315 ACCTGGT A^CC[AT]GGT
1481 ACCTGGT
SmlI 1843 CTCAAG C^T[CT][GA]AG
2179 CTTAAG
TfiI 704 GAATC G^A[AT]TC
2675 GAATC
XmnI 1644 GAACCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2556 GAACATCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 104 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI ApoI AvaI AvaII
BalI BanI BbsI BbvI BcnI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaBI BsaHI BsaI BsaJI BseRI
BsgI BsiEI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrI
BssKI BstF5I BstNI BstUI BstYI
Cac8I CelII CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeII MaeIII MamI MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV PleI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
StyI TaqI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII